72d423234f2cbde3373b70d6cfbc1075 311614.pdf 331eedf2eb692194e520ac5c5c2a33182f584018 311614.pdf 64f6df251a004326e951d4042ab0b7f119bc88aef2d2a8e3d1838c0f68292ce1 311614.pdf Title: Deep-amplicon sequencing of the complete beta-tubulin gene in Trichuris trichiura before and after albendazole treatment Subject: International Journal for Parasitology: Drugs and Drug Resistance, 26 (2024) 100570. doi:10.1016/j.ijpddr.2024.100570 Keywords: Trichuris trichiura,Deep-amplicon sequencing,Beta-tubulin,Benzimidazole resistance,Genomics Author: Javier Gandasegui Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Wed Dec 11 00:45:35 2024 CET ModDate: Wed Dec 11 04:34:16 2024 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 2499817 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZWVJAZ+CharisSIL CID TrueType Identity-H yes yes yes 577 0 AZADWJ+CharisSIL CID TrueType Identity-H yes yes yes 583 0 SEWCYO+Univers Type 1C Builtin yes yes yes 588 0 POXKRZ+CharisSIL-Italic CID TrueType Identity-H yes yes yes 592 0 GUEYNC+STIX-Regular Type 1C Custom yes yes yes 597 0 BAFALN+TeX_CM_Roman Type 1C Builtin yes yes yes 602 0 AAAWKD+CharisSIL CID TrueType Identity-H yes yes yes 606 0 CLWGKL+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 614 0 OKLRLA+CharisSIL-Italic CID TrueType Identity-H yes yes yes 395 0 LYXWUV+CharisSIL-Bold CID TrueType Identity-H yes yes yes 401 0 AAAUKC+CharisSIL-Italic CID TrueType Identity-H yes yes yes 406 0 AAAAAD+CharisSIL CID TrueType Identity-H yes yes yes 412 0 KUGFAH+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 419 0 TWBIRU+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 74 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-06-26 02:38:44 CEST RepresentationInformation: 311614.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-06-25 14:16:15 CEST Size: 2499817 Format: PDF Version: 1.7 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ErrorMessage: Invalid indirect destination - referenced object 'appsec1' cannot be found ID: PDF-HUL-149 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found ID: PDF-HUL-122 Offset: 2477904 MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 714 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Deep-amplicon sequencing of the complete beta-tubulin gene in Trichuris trichiura before and after albendazole treatment Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Material and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Ethics approval and consent to participate Destination: mk:H2_1 Item: Title: 2.2 Study population, sample collection and processing Destination: mk:H2_2 Item: Title: 2.3 PCR amplification and sequencing Destination: mk:H2_3 Item: Title: 2.4 Sequencing data processing and analysis Destination: mk:H2_4 Children: Item: Title: 2.4.1 Mapping Destination: mk:H3_5 Item: Title: 2.4.2 Analysis of variation in the beta-tubulin gene Destination: mk:H3_6 Item: Title: 2.4.3 Evaluating the second beta-tubulin isotype in drug resistance Destination: mk:H3_7 Item: Title: Availability of data and materials Destination: mk:H3_8 Item: Title: 3 Results and discussion Destination: mk:H1_3 Item: Title: 4 Conclusion Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_16 Item: Title: Acknowledgements Destination: mk:HH1_17 Item: Title: Appendix A Supplementary data Destination: mk:H1_6 Item: Title: References Destination: mk:HH2_1 Info: Title: Deep-amplicon sequencing of the complete beta-tubulin gene in Trichuris trichiura before and after albendazole treatment Author: Javier Gandasegui Subject: International Journal for Parasitology: Drugs and Drug Resistance, 26 (2024) 100570. doi:10.1016/j.ijpddr.2024.100570 Keywords: Trichuris trichiura,Deep-amplicon sequencing,Beta-tubulin,Benzimidazole resistance,Genomics Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Wed Dec 11 00:45:35 CET 2024 ModDate: Wed Dec 11 04:34:16 CET 2024 ID: 0x35a6e8ddce6a1d2c5b6a57c6a365a95d, 0x1f2944c98c81e6a7612bee9e189844d9 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: POXKRZ+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: ZWVJAZ+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: LYXWUV+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: KUGFAH+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: AAAUKC+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: AZADWJ+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: TWBIRU+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: OKLRLA+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: AAAAAD+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: AAAWKD+CharisSIL Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: GUEYNC+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 10 FontDescriptor: FontName: GUEYNC+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: CLWGKL+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: CLWGKL+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: BAFALN+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: BAFALN+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: SEWCYO+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: SEWCYO+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true CIDFontType2: Font: BaseFont: AAAWKD+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: AAAWKD+CharisSIL Flags: Symbolic FontBBox: -692, -533, 1623, 1318 FontFile2: true Font: BaseFont: POXKRZ+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: POXKRZ+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: ZWVJAZ+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: ZWVJAZ+CharisSIL Flags: Serif, Symbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: LYXWUV+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: LYXWUV+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true Font: BaseFont: KUGFAH+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: KUGFAH+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: AAAUKC+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: AAAUKC+CharisSIL-Italic Flags: Symbolic, Italic FontBBox: -716, -533, 1626, 1317 FontFile2: true Font: BaseFont: AZADWJ+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: AZADWJ+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: TWBIRU+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: TWBIRU+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2046, 1040 FontFile2: true Font: BaseFont: OKLRLA+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: OKLRLA+CharisSIL-Italic Flags: Serif, Symbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: AAAAAD+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: AAAAAD+CharisSIL Flags: Symbolic FontBBox: -692, -533, 1623, 1318 FontFile2: true XMP: http://creativecommons.org/licenses/by/4.0/ elsevier.com sciencedirect.com true 10.1016/j.ijpddr.2024.100570 2010-04-23 application/pdf 10.1016/j.ijpddr.2024.100570 Elsevier Ltd International Journal for Parasitology: Drugs and Drug Resistance, 26 (2024) 100570. doi:10.1016/j.ijpddr.2024.100570 Trichuris trichiura Deep-amplicon sequencing Beta-tubulin Benzimidazole resistance Genomics Deep-amplicon sequencing of the complete beta-tubulin gene in Trichuris trichiura before and after albendazole treatment Javier Gandasegui Berta Grau-Pujol Valdemiro Novela Osvaldo Muchisse Maria Cambra-Pellejà Anélsio Cossa José Carlos Jamine Charfudin Sacoor Eric A.T. Brienen Francesc Catala-Moll Lisette van Lieshout María Martínez-Valladares Roger Paredes José Muñoz Stephen R. Doyle VoR 11th December 2024 Acrobat Distiller 8.1.0 (Windows) Trichuris trichiura,Deep-amplicon sequencing,Beta-tubulin,Benzimidazole resistance,Genomics 11th December 2024 sciencedirect.com elsevier.com true 2010-04-23 10.1016/j.ijpddr.2024.100570 noindex journal © 2024 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. 2024-12-01 1 December 2024 10.1016/j.ijpddr.2024.100570 2211-3207 100570 International Journal for Parasitology: Drugs and Drug Resistance 100570 https://doi.org/10.1016/j.ijpddr.2024.100570 26 https://www.elsevier.com/tdm/tdmrep-policy.json 1 2024-12-10T23:45:35 Elsevier 2024-12-11T03:34:16 2024-12-11T03:34:16 uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 True Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 125, 576, 129, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 132, 576, 136, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 139, 576, 143, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 230, 576, 234, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 237, 576, 240, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 243, 576, 247, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 341, 576, 344, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 437, 576, 441, 587 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 141, 563, 145, 574 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 148, 563, 151, 574 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 154, 563, 157, 574 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 229, 563, 233, 574 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 332, 563, 336, 574 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 426, 563, 430, 574 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 120, 550, 124, 561 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 230, 550, 235, 561 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 338, 550, 342, 561 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 475, 550, 478, 561 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 106, 537, 111, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 113, 537, 115, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 118, 537, 122, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 125, 537, 127, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 130, 537, 136, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 200, 537, 205, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 295, 537, 299, 548 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 103, 133, 164, 140 Flags: Print Annotation: Subtype: Link Rect: 221, 133, 295, 140 Flags: Print Annotation: Subtype: Link Rect: 350, 133, 451, 140 Flags: Print Annotation: Subtype: Link Rect: 495, 133, 557, 140 Flags: Print Annotation: Subtype: Link Rect: 37, 123, 76, 130 Flags: Print Annotation: Subtype: Link Rect: 136, 123, 224, 130 Flags: Print Annotation: Subtype: Link Rect: 304, 123, 390, 130 Flags: Print Annotation: Subtype: Link Rect: 438, 123, 504, 130 Flags: Print Annotation: Subtype: Link Rect: 37, 114, 136, 121 Flags: Print Annotation: Subtype: Link Rect: 184, 114, 258, 121 Flags: Print Annotation: Subtype: Link Rect: 322, 114, 385, 121 Flags: Print Annotation: Subtype: Link Rect: 448, 114, 529, 121 Flags: Print Annotation: Subtype: Link Rect: 71, 104, 136, 111 Flags: Print Annotation: Subtype: Link Rect: 221, 104, 290, 111 Flags: Print Annotation: Subtype: Link Rect: 334, 104, 414, 111 Flags: Print Annotation: Subtype: Link Rect: 454, 104, 515, 111 Flags: Print Annotation: Subtype: Link Rect: 321, 722, 369, 730 Flags: Print Annotation: Subtype: Link Rect: 276, 664, 395, 672 Flags: Print Annotation: Subtype: Link Rect: 37, 87, 186, 94 Flags: Print Annotation: Subtype: Link Rect: 297, 749, 300, 757 Flags: Print Annotation: Subtype: Link Rect: 162, 748, 432, 760 Annotation: Subtype: Link Rect: 501, 616, 529, 644 Annotation: Subtype: Link Rect: 40, 49, 186, 61 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 210, 618, 288, 626 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 37, 608, 86, 616 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 90, 608, 113, 616 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 40, 556, 121, 564 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 98, 514, 161, 522 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 81, 493, 146, 501 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 151, 493, 217, 501 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 40, 441, 113, 449 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 229, 399, 286, 407 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 37, 388, 139, 396 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 146, 388, 211, 396 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 217, 388, 288, 396 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 37, 378, 77, 386 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 82, 378, 149, 386 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 208, 325, 283, 333 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 88, 294, 170, 302 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 175, 294, 255, 302 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 260, 294, 288, 302 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 37, 284, 78, 292 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 122, 263, 202, 271 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 91, 179, 179, 187 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 259, 127, 288, 135 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 37, 116, 80, 124 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 77, 85, 144, 93 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 452, 460, 530, 468 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 534, 460, 552, 468 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 309, 334, 388, 342 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 417, 144, 504, 152 Flags: Print Destination: 311 Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 61, 510, 195, 518 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 210, 479, 288, 487 Flags: Print Annotation: Subtype: Link Rect: 37, 468, 215, 476 Flags: Print Annotation: Subtype: Link Rect: 67, 458, 96, 466 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 61, 437, 129, 445 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 118, 426, 188, 434 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 190, 416, 253, 424 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 57, 364, 120, 372 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 64, 259, 120, 267 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 132, 113, 197, 121 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 309, 678, 380, 686 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 513, 626, 557, 634 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 306, 615, 328, 623 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 458, 542, 532, 550 Flags: Print Annotation: Subtype: Link Rect: 540, 511, 557, 519 Flags: Print Annotation: Subtype: Link Rect: 306, 500, 459, 508 Flags: Print Annotation: Subtype: Link Rect: 481, 426, 541, 434 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 546, 426, 557, 434 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 306, 416, 400, 424 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 406, 416, 472, 424 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 309, 364, 353, 372 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 458, 322, 523, 330 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 493, 311, 557, 319 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 306, 301, 324, 309 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 329, 301, 401, 309 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 495, 301, 557, 309 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 306, 290, 324, 298 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 468, 186, 532, 194 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 538, 186, 557, 194 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 306, 175, 346, 183 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 351, 175, 419, 183 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 424, 175, 483, 183 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 309, 102, 340, 110 Flags: Print Destination: null Page: Label: 4 Annotations: Annotation: Subtype: Link Rect: 194, 709, 220, 717 Flags: Print Destination: 71 Annotation: Subtype: Link Rect: 98, 689, 128, 696 Flags: Print Destination: null Annotation: Subtype: Link Rect: 176, 647, 196, 655 Flags: Print Destination: 101 Annotation: Subtype: Link Rect: 165, 636, 186, 644 Flags: Print Destination: 101 Annotation: Subtype: Link Rect: 207, 626, 271, 634 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 87, 542, 118, 550 Flags: Print Destination: null Annotation: Subtype: Link Rect: 154, 448, 175, 456 Flags: Print Destination: 101 Annotation: Subtype: Link Rect: 129, 427, 148, 435 Flags: Print Destination: 101 Annotation: Subtype: Link Rect: 416, 709, 507, 717 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 513, 709, 557, 717 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 306, 699, 324, 707 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 335, 657, 355, 665 Flags: Print Destination: 101 Annotation: Subtype: Link Rect: 465, 511, 534, 519 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 358, 458, 441, 466 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 435, 427, 457, 435 Flags: Print Destination: 128 Annotation: Subtype: Link Rect: 419, 343, 441, 351 Flags: Print Destination: 128 Annotation: Subtype: Link Rect: 378, 312, 440, 320 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 467, 270, 534, 278 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 335, 176, 399, 184 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 466, 50, 544, 58 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 262, 339, 264, 345 Flags: Print Destination: 71 Annotation: Subtype: Link Rect: 136, 205, 141, 212 Flags: Print Destination: 71 Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 135, 334, 231, 342 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 238, 334, 288, 342 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 37, 324, 78, 332 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 40, 303, 122, 311 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 126, 303, 207, 311 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 212, 303, 283, 311 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 60, 219, 139, 227 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 144, 136, 242, 143 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 215, 104, 286, 112 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 37, 94, 127, 102 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 134, 94, 206, 102 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 63, 52, 132, 60 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 138, 52, 209, 60 Flags: Print Destination: 311 Annotation: Subtype: Link Rect: 215, 52, 283, 60 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 379, 324, 453, 332 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 459, 324, 532, 332 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 537, 324, 555, 332 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 306, 313, 416, 321 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 524, 188, 557, 196 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 306, 177, 324, 185 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 328, 177, 397, 185 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 352, 146, 436, 154 Flags: Print Destination: 132 Annotation: Subtype: Link Rect: 458, 125, 521, 133 Flags: Print Destination: 311 Page: Label: 6 Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 247, 532, 288, 540 Flags: Print Annotation: Subtype: Link Rect: 37, 521, 161, 529 Flags: Print Annotation: Subtype: Link Rect: 37, 482, 262, 489 Flags: Print Annotation: Subtype: Link Rect: 49, 474, 286, 481 Flags: Print Annotation: Subtype: Link Rect: 49, 466, 283, 473 Flags: Print Annotation: Subtype: Link Rect: 49, 458, 67, 465 Flags: Print Annotation: Subtype: Link Rect: 37, 450, 278, 457 Flags: Print Annotation: Subtype: Link Rect: 49, 442, 286, 449 Flags: Print Annotation: Subtype: Link Rect: 49, 434, 281, 441 Flags: Print Annotation: Subtype: Link Rect: 37, 427, 286, 433 Flags: Print Annotation: Subtype: Link Rect: 49, 419, 107, 425 Flags: Print Annotation: Subtype: Link Rect: 37, 411, 271, 417 Flags: Print Annotation: Subtype: Link Rect: 49, 403, 286, 409 Flags: Print Annotation: Subtype: Link Rect: 49, 395, 265, 401 Flags: Print Annotation: Subtype: Link Rect: 49, 387, 286, 393 Flags: Print Annotation: Subtype: Link Rect: 49, 379, 271, 385 Flags: Print Annotation: Subtype: Link Rect: 49, 371, 258, 377 Flags: Print Annotation: Subtype: Link Rect: 49, 363, 285, 369 Flags: Print Annotation: Subtype: Link Rect: 49, 355, 131, 361 Flags: Print Annotation: Subtype: Link Rect: 37, 347, 267, 353 Flags: Print Annotation: Subtype: Link Rect: 49, 339, 284, 345 Flags: Print Annotation: Subtype: Link Rect: 37, 331, 253, 337 Flags: Print Annotation: Subtype: Link Rect: 49, 323, 286, 329 Flags: Print Annotation: Subtype: Link Rect: 49, 315, 163, 321 Flags: Print Annotation: Subtype: Link Rect: 37, 307, 286, 313 Flags: Print Annotation: Subtype: Link Rect: 49, 299, 267, 305 Flags: Print Annotation: Subtype: Link Rect: 49, 291, 275, 297 Flags: Print Annotation: Subtype: Link Rect: 49, 283, 257, 289 Flags: Print Annotation: Subtype: Link Rect: 37, 275, 279, 281 Flags: Print Annotation: Subtype: Link Rect: 49, 267, 177, 273 Flags: Print Annotation: Subtype: Link Rect: 37, 259, 249, 266 Flags: Print Annotation: Subtype: Link Rect: 49, 251, 275, 258 Flags: Print Annotation: Subtype: Link Rect: 49, 243, 286, 250 Flags: Print Annotation: Subtype: Link Rect: 49, 235, 272, 242 Flags: Print Annotation: Subtype: Link Rect: 49, 227, 179, 234 Flags: Print Annotation: Subtype: Link Rect: 37, 219, 266, 226 Flags: Print Annotation: Subtype: Link Rect: 49, 211, 268, 218 Flags: Print Annotation: Subtype: Link Rect: 49, 203, 286, 210 Flags: Print Annotation: Subtype: Link Rect: 49, 195, 277, 202 Flags: Print Annotation: Subtype: Link Rect: 49, 187, 146, 194 Flags: Print Annotation: Subtype: Link Rect: 37, 179, 286, 186 Flags: Print Annotation: Subtype: Link Rect: 49, 171, 113, 178 Flags: Print Annotation: Subtype: Link Rect: 37, 163, 280, 170 Flags: Print Annotation: Subtype: Link Rect: 49, 156, 263, 162 Flags: Print Annotation: Subtype: Link Rect: 49, 148, 81, 154 Flags: Print Annotation: Subtype: Link Rect: 37, 140, 270, 146 Flags: Print Annotation: Subtype: Link Rect: 49, 132, 275, 138 Flags: Print Annotation: Subtype: Link Rect: 49, 124, 254, 130 Flags: Print Annotation: Subtype: Link Rect: 49, 116, 278, 122 Flags: Print Annotation: Subtype: Link Rect: 49, 108, 154, 114 Flags: Print Annotation: Subtype: Link Rect: 37, 100, 261, 106 Flags: Print Annotation: Subtype: Link Rect: 49, 92, 281, 98 Flags: Print Annotation: Subtype: Link Rect: 49, 84, 286, 90 Flags: Print Annotation: Subtype: Link Rect: 49, 76, 125, 82 Flags: Print Annotation: Subtype: Link Rect: 37, 68, 281, 74 Flags: Print Annotation: Subtype: Link Rect: 49, 60, 268, 66 Flags: Print Annotation: Subtype: Link Rect: 318, 732, 534, 738 Flags: Print Annotation: Subtype: Link Rect: 318, 724, 516, 730 Flags: Print Annotation: Subtype: Link Rect: 306, 716, 555, 722 Flags: Print Annotation: Subtype: Link Rect: 318, 708, 523, 714 Flags: Print Annotation: Subtype: Link Rect: 318, 700, 547, 706 Flags: Print Annotation: Subtype: Link Rect: 318, 692, 515, 698 Flags: Print Annotation: Subtype: Link Rect: 306, 684, 555, 690 Flags: Print Annotation: Subtype: Link Rect: 318, 676, 552, 682 Flags: Print Annotation: Subtype: Link Rect: 318, 668, 554, 674 Flags: Print Annotation: Subtype: Link Rect: 318, 660, 387, 666 Flags: Print Annotation: Subtype: Link Rect: 306, 652, 555, 658 Flags: Print Annotation: Subtype: Link Rect: 318, 644, 555, 651 Flags: Print Annotation: Subtype: Link Rect: 318, 636, 545, 643 Flags: Print Annotation: Subtype: Link Rect: 318, 628, 520, 635 Flags: Print Annotation: Subtype: Link Rect: 318, 620, 542, 627 Flags: Print Annotation: Subtype: Link Rect: 318, 612, 424, 619 Flags: Print Annotation: Subtype: Link Rect: 306, 604, 554, 611 Flags: Print Annotation: Subtype: Link Rect: 318, 596, 545, 603 Flags: Print Annotation: Subtype: Link Rect: 318, 588, 471, 595 Flags: Print Annotation: Subtype: Link Rect: 306, 580, 530, 587 Flags: Print Annotation: Subtype: Link Rect: 318, 572, 459, 579 Flags: Print Annotation: Subtype: Link Rect: 306, 565, 529, 571 Flags: Print Annotation: Subtype: Link Rect: 318, 557, 546, 563 Flags: Print Annotation: Subtype: Link Rect: 318, 549, 536, 555 Flags: Print Annotation: Subtype: Link Rect: 318, 541, 536, 547 Flags: Print Annotation: Subtype: Link Rect: 318, 533, 555, 539 Flags: Print Annotation: Subtype: Link Rect: 318, 525, 467, 531 Flags: Print Annotation: Subtype: Link Rect: 306, 517, 552, 523 Flags: Print Annotation: Subtype: Link Rect: 318, 509, 555, 515 Flags: Print Annotation: Subtype: Link Rect: 318, 501, 534, 507 Flags: Print Annotation: Subtype: Link Rect: 318, 493, 545, 499 Flags: Print Annotation: Subtype: Link Rect: 318, 485, 555, 491 Flags: Print Annotation: Subtype: Link Rect: 318, 477, 357, 483 Flags: Print Annotation: Subtype: Link Rect: 306, 469, 555, 475 Flags: Print Annotation: Subtype: Link Rect: 318, 461, 549, 467 Flags: Print Annotation: Subtype: Link Rect: 318, 453, 553, 459 Flags: Print Annotation: Subtype: Link Rect: 306, 445, 546, 451 Flags: Print Annotation: Subtype: Link Rect: 318, 437, 555, 443 Flags: Print Annotation: Subtype: Link Rect: 318, 429, 537, 435 Flags: Print Annotation: Subtype: Link Rect: 318, 421, 548, 427 Flags: Print Annotation: Subtype: Link Rect: 318, 413, 555, 419 Flags: Print Annotation: Subtype: Link Rect: 318, 405, 343, 411 Flags: Print Annotation: Subtype: Link Rect: 306, 397, 555, 403 Flags: Print Annotation: Subtype: Link Rect: 318, 389, 539, 396 Flags: Print Annotation: Subtype: Link Rect: 318, 381, 546, 388 Flags: Print Annotation: Subtype: Link Rect: 318, 373, 555, 380 Flags: Print Annotation: Subtype: Link Rect: 318, 365, 386, 372 Flags: Print Annotation: Subtype: Link Rect: 306, 357, 555, 364 Flags: Print Annotation: Subtype: Link Rect: 318, 349, 521, 356 Flags: Print Annotation: Subtype: Link Rect: 318, 341, 464, 348 Flags: Print Annotation: Subtype: Link Rect: 306, 333, 531, 340 Flags: Print Annotation: Subtype: Link Rect: 318, 325, 554, 332 Flags: Print Annotation: Subtype: Link Rect: 318, 317, 537, 324 Flags: Print Annotation: Subtype: Link Rect: 318, 309, 456, 316 Flags: Print Annotation: Subtype: Link Rect: 306, 301, 532, 308 Flags: Print Annotation: Subtype: Link Rect: 318, 294, 555, 300 Flags: Print Annotation: Subtype: Link Rect: 318, 286, 554, 292 Flags: Print Annotation: Subtype: Link Rect: 318, 278, 411, 284 Flags: Print Annotation: Subtype: Link Rect: 306, 270, 543, 276 Flags: Print Annotation: Subtype: Link Rect: 318, 262, 543, 268 Flags: Print Annotation: Subtype: Link Rect: 318, 254, 554, 260 Flags: Print Annotation: Subtype: Link Rect: 318, 246, 555, 252 Flags: Print Annotation: Subtype: Link Rect: 318, 238, 536, 244 Flags: Print Annotation: Subtype: Link Rect: 306, 230, 555, 236 Flags: Print Annotation: Subtype: Link Rect: 318, 222, 436, 228 Flags: Print Annotation: Subtype: Link Rect: 306, 214, 549, 220 Flags: Print Annotation: Subtype: Link Rect: 318, 206, 530, 212 Flags: Print Annotation: Subtype: Link Rect: 318, 198, 549, 204 Flags: Print Annotation: Subtype: Link Rect: 318, 190, 555, 196 Flags: Print Annotation: Subtype: Link Rect: 318, 182, 363, 188 Flags: Print Annotation: Subtype: Link Rect: 306, 174, 546, 180 Flags: Print Annotation: Subtype: Link Rect: 318, 166, 550, 172 Flags: Print Annotation: Subtype: Link Rect: 318, 158, 540, 164 Flags: Print Annotation: Subtype: Link Rect: 318, 150, 458, 156 Flags: Print Annotation: Subtype: Link Rect: 306, 142, 533, 148 Flags: Print Annotation: Subtype: Link Rect: 318, 134, 545, 140 Flags: Print Annotation: Subtype: Link Rect: 318, 126, 555, 133 Flags: Print Annotation: Subtype: Link Rect: 318, 118, 548, 125 Flags: Print Annotation: Subtype: Link Rect: 318, 110, 554, 117 Flags: Print Annotation: Subtype: Link Rect: 318, 102, 362, 109 Flags: Print Annotation: Subtype: Link Rect: 306, 94, 533, 101 Flags: Print Annotation: Subtype: Link Rect: 318, 86, 542, 93 Flags: Print Annotation: Subtype: Link Rect: 318, 78, 541, 85 Flags: Print Annotation: Subtype: Link Rect: 318, 70, 512, 77 Flags: Print Annotation: Subtype: Link Rect: 306, 62, 549, 69 Flags: Print Annotation: Subtype: Link Rect: 318, 54, 555, 61 Flags: Print Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 49, 732, 273, 738 Flags: Print Annotation: Subtype: Link Rect: 49, 724, 286, 730 Flags: Print Annotation: Subtype: Link Rect: 49, 716, 286, 722 Flags: Print Annotation: Subtype: Link Rect: 49, 708, 121, 714 Flags: Print Annotation: Subtype: Link Rect: 37, 700, 282, 706 Flags: Print Annotation: Subtype: Link Rect: 49, 692, 276, 698 Flags: Print Annotation: Subtype: Link Rect: 49, 684, 63, 690 Flags: Print Annotation: Subtype: Link Rect: 37, 676, 276, 682 Flags: Print Annotation: Subtype: Link Rect: 49, 668, 286, 674 Flags: Print Annotation: Subtype: Link Rect: 49, 660, 269, 666 Flags: Print Annotation: Subtype: Link Rect: 49, 652, 286, 658 Flags: Print Annotation: Subtype: Link Rect: 49, 644, 66, 651 Flags: Print Annotation: Subtype: Link Rect: 37, 636, 267, 643 Flags: Print Annotation: Subtype: Link Rect: 49, 628, 286, 635 Flags: Print Annotation: Subtype: Link Rect: 49, 620, 95, 627 Flags: Print Annotation: Subtype: Link Rect: 37, 612, 286, 619 Flags: Print Annotation: Subtype: Link Rect: 49, 604, 277, 611 Flags: Print Annotation: Subtype: Link Rect: 49, 596, 117, 603 Flags: Print Annotation: Subtype: Link Rect: 37, 588, 262, 595 Flags: Print Annotation: Subtype: Link Rect: 49, 580, 257, 587 Flags: Print Annotation: Subtype: Link Rect: 49, 572, 262, 579 Flags: Print Annotation: Subtype: Link Rect: 49, 565, 261, 571 Flags: Print Annotation: Subtype: Link Rect: 49, 557, 281, 563 Flags: Print Annotation: Subtype: Link Rect: 49, 549, 226, 555 Flags: Print Annotation: Subtype: Link Rect: 37, 541, 279, 547 Flags: Print Annotation: Subtype: Link Rect: 49, 533, 258, 539 Flags: Print Annotation: Subtype: Link Rect: 49, 525, 191, 531 Flags: Print Annotation: Subtype: Link Rect: 37, 517, 274, 523 Flags: Print Annotation: Subtype: Link Rect: 49, 509, 275, 515 Flags: Print Annotation: Subtype: Link Rect: 49, 501, 286, 507 Flags: Print Annotation: Subtype: Link Rect: 49, 493, 282, 499 Flags: Print Annotation: Subtype: Link Rect: 49, 485, 77, 491 Flags: Print Annotation: Subtype: Link Rect: 306, 732, 533, 738 Flags: Print Annotation: Subtype: Link Rect: 318, 724, 536, 730 Flags: Print Annotation: Subtype: Link Rect: 318, 716, 532, 722 Flags: Print Annotation: Subtype: Link Rect: 318, 708, 464, 714 Flags: Print Annotation: Subtype: Link Rect: 306, 700, 555, 706 Flags: Print Annotation: Subtype: Link Rect: 318, 692, 550, 698 Flags: Print Annotation: Subtype: Link Rect: 318, 684, 393, 690 Flags: Print Annotation: Subtype: Link Rect: 306, 676, 555, 682 Flags: Print Annotation: Subtype: Link Rect: 318, 668, 539, 674 Flags: Print Annotation: Subtype: Link Rect: 318, 660, 386, 666 Flags: Print Annotation: Subtype: Link Rect: 306, 652, 542, 658 Flags: Print Annotation: Subtype: Link Rect: 318, 644, 514, 651 Flags: Print Annotation: Subtype: Link Rect: 306, 636, 554, 643 Flags: Print Annotation: Subtype: Link Rect: 318, 628, 533, 635 Flags: Print Annotation: Subtype: Link Rect: 318, 620, 347, 627 Flags: Print Annotation: Subtype: Link Rect: 306, 612, 547, 619 Flags: Print Annotation: Subtype: Link Rect: 318, 604, 461, 611 Flags: Print Annotation: Subtype: Link Rect: 306, 596, 541, 603 Flags: Print Annotation: Subtype: Link Rect: 318, 588, 551, 595 Flags: Print Annotation: Subtype: Link Rect: 318, 580, 545, 587 Flags: Print Annotation: Subtype: Link Rect: 318, 572, 549, 579 Flags: Print Annotation: Subtype: Link Rect: 318, 565, 407, 571 Flags: Print Annotation: Subtype: Link Rect: 306, 557, 544, 563 Flags: Print Annotation: Subtype: Link Rect: 318, 549, 553, 555 Flags: Print Annotation: Subtype: Link Rect: 318, 541, 555, 547 Flags: Print Annotation: Subtype: Link Rect: 318, 533, 357, 539 Flags: Print Annotation: Subtype: Link Rect: 306, 525, 536, 531 Flags: Print Annotation: Subtype: Link Rect: 318, 517, 555, 523 Flags: Print Annotation: Subtype: Link Rect: 318, 509, 376, 515 Flags: Print Annotation: Subtype: Link Rect: 306, 501, 547, 507 Flags: Print Annotation: Subtype: Link Rect: 318, 493, 458, 499 Flags: Print Checksum: 2d3a8fc4 Type: CRC32 Checksum: 72d423234f2cbde3373b70d6cfbc1075 Type: MD5 Checksum: 331eedf2eb692194e520ac5c5c2a33182f584018 Type: SHA-1 Checksum: 64f6df251a004326e951d4042ab0b7f119bc88aef2d2a8e3d1838c0f68292ce1 Type: SHA-256