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Title:           Insights into the human cDNA: A descriptive study using library screening in yeast
Subject:         Journal of Genetic Engineering and Biotechnology, 22 (2024) 100427. doi:10.1016/j.jgeb.2024.100427
Keywords:        cDNA library screens,Yeast two-hybrid,Saccharomyces cerevisiae,Genetic screens
Author:          Zina Alaswad
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      Title: Insights into the human cDNA: A descriptive study using library screening in yeast
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 The cDNA library
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          Title: 2.2 Yeast screening
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          Title: 2.3 Random selection of cDNA library plasmids
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          Title: 2.4 Data analysis
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        Title: 3 Results
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          Title: 3.1 5′ end truncated cDNAs are enriched in the library
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          Title: 3.2 Internal priming for mRNA sequences generates truncated cDNAs among the library transcripts
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          Title: 3.3 Several identified CDS transcripts were not fused to GAL4 AD
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          Title: 3.4 Multiple Sanger sequenced reads aligned to non-coding regions
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          Title: 3.5 Mitochondrial transcripts are highly enriched in the library
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        Title: 4 Discussion
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          Title: 4.1 General recommendations for cDNA library construction
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          Title: 4.2 The future of genetic screens
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        Title: 5 Conclusion
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        Title: Funding
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        Title: CRediT authorship contribution statement
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        Title: Declaration of Competing Interest
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        Title: Acknowledgement
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        Title: Appendix A Supplementary material
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        Title: References
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    Author: Zina Alaswad
    Subject: Journal of Genetic Engineering and Biotechnology, 22 (2024) 100427. doi:10.1016/j.jgeb.2024.100427
    Keywords: cDNA library screens,Yeast two-hybrid,Saccharomyces cerevisiae,Genetic screens
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   Type: CRC32
  Checksum: 60774841bf2da917129d0587303bc616
   Type: MD5
  Checksum: baaab87b7e8553c26154fd118efecfcb8d6a8c7b
   Type: SHA-1
  Checksum: 077b7fb8266b8a052e10e82945be58726b63e4c7347983059cb69a519350b22c
   Type: SHA-256