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CEST Size: 5620305 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 946 FreeObjects: 4 IncrementalUpdates: 2 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Role of SARS-CoV-2-specific memory B cells promoting immune protection after booster vaccination in solid organ transplantation Destination: bmark_461755168 Children: Item: Title: 1 Introduction Destination: s1 Item: Title: 2 Materials and methods Destination: s2 Children: Item: Title: 2.1 Patients of the study and clinical definitions Destination: s2_1 Children: Item: Title: 2.1.1 Observational cohort Destination: s2_1_1 Children: Item: Title: 2.1.1.1 SARS-CoV-2 vaccination Destination: s2_1_1_1 Item: Title: 2.1.1.2 COVID-19 breakthrough infections Destination: s2_1_1_2 Item: Title: 2.1.2 Randomized controlled trial (Tor-vax) Destination: s2_1_2 Children: Item: Title: 2.1.2.1 Main endpoints of the study and sample size Destination: s2_1_2_1 Item: Title: 2.1.2.2 Inclusion criteria Destination: s2_1_2_2 Item: Title: 2.1.2.3 Exclusion criteria Destination: s2_1_2_3 Item: Title: 2.2 Collection and management of biological samples Destination: s2_2 Item: Title: 2.3 Assessment of SARS-CoV-2-specific adaptive immune memory Destination: s2_3 Children: Item: Title: 2.3.1 SARS-CoV-2-specific humoral memory Destination: s2_3_1 Children: Item: Title: 2.3.1.1 SARS-CoV-2-specific serological memory Destination: s2_3_1_1 Children: Item: Title: 2.3.1.1.1 SARS-CoV-2-specific serum IgG antibodies Destination: s2_3_1_1_1 Item: Title: 2.3.1.1.2 SARS-CoV-2-specific neutralizing antibodies Destination: s2_3_1_1_2 Item: Title: 2.3.1.2 RBD-specific spike IgG-producing memory B cells Destination: s2_3_1_2 Item: Title: 2.3.2 SARS-CoV-2-reactive cytokine-producing memory T cells Destination: s2_3_2 Item: Title: 2.3.3 Analysis of immune memory compartments Destination: s2_3_3 Item: Title: 2.4 Statistics Destination: s2_4 Item: Title: 3 Results Destination: s3 Children: Item: Title: 3.1 Patients and outcomes of the observational cohort Destination: s3_1 Item: Title: 3.2 Strength and kinetics of adaptive immune memory after booster vaccination Destination: s3_2 Item: Title: 3.3 Neutralizing cross-reactivity of serum and IgG-producing RBD-specific mBc Destination: s3_3 Item: Title: 3.4 Immunosuppression regimens and immune memory after vaccination Destination: s3_4 Item: Title: 3.5 RBD-specific memory B cells predict NAb seroconversion after booster vaccination and protection from severe COVID-19 Destination: s3_5 Item: Title: 3.6 RBD-specific mBC and changes in immunosuppression favor successful immunization in nonresponders Destination: s3_6 Item: Title: 4 Discussion Destination: s4 Item: Title: Data availability statement Destination: bmark_-1258588262 Item: Title: Ethics statement Destination: bmark_1370551237 Item: Title: Author contributions Destination: bmark_-1208584890 Item: Title: Funding 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Castells Maria F. Cortese Juliana Esperalba Candela Fernández-Naval Jesús Quintero Marina Muñoz Fernando Agüero José Gonzalez-Costello Laura Lladó Alexandre Favà Laura Cañas María del Mar de la Hoz-Caballero Maria Meneghini Irina B. Torres Mariona Juvé FMJ Hafkamp Marta Vila Alba G. Robles Maria José Buzón Nestor Toapanta José Miguel Zúñiga Víctor Monforte Berta Saez-Giménez Oscar Len Ibai Los Arcos Enric Miret Gema Ariceta Emma Pardo Xavier Martínez Francesc Moreso Oriol Bestard Donadeu L, Gomez-Olles S, Casanova F, Torija A, Lopez-Meseguer M, Boada-Pérez M, Kervella D, Crespo E, Carrera-Muñoz C, Campos-Varela I, Castells L, Cortese MF, Esperalba J, Fernández-Naval C, Quintero J, Muñoz M, Agüero F, Gonzalez-Costello J, Lladó L, Favà A, Cañas L, del Mar de la Hoz-Caballero M, Meneghini M, Torres IB, Juvé M, Hafkamp FMJ, Vila M, Robles AG, Buzón MJ, Toapanta N, Zúñiga JM, Monforte V, Saez-Giménez B, Len O, Arcos IL, Miret E, Ariceta G, Pardo E, Martínez X, Moreso F and Bestard O (2024) Role of SARS-CoV-2-specific memory B cells promoting immune protection after booster vaccination in solid organ transplantation. Front. Immunol. 15:1463769. doi: 10.3389/fimmu.2024.1463769 Copyright © 2024 Donadeu, Gomez-Olles, Casanova, Torija, Lopez-Meseguer, Boada-Pérez, Kervella, Crespo, Carrera-Muñoz, Campos-Varela, Castells, Cortese, Esperalba, Fernández-Naval, Quintero, Muñoz, Agüero, Gonzalez-Costello, Lladó, Favà, Cañas, del Mar de la Hoz-Caballero, Meneghini, Torres, Juvé, Hafkamp, Vila, Robles, Buzón, Toapanta, Zúñiga, Monforte, Saez-Giménez, Len, Arcos, Miret, Ariceta, Pardo, Martínez, Moreso and Bestard. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. SARS-CoV-2 booster vaccination solid organ transplantation adaptive immunity neutralizing antibodies Role of SARS-CoV-2-specific memory B cells promoting immune protection after booster vaccination in solid organ transplantation SARS-CoV-2; booster vaccination; solid organ transplantation; adaptive immunity; neutralizing antibodies True https://www.frontiersin.org/legal/copyright-statement Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 221, 695, 502, 716 Annotation: Subtype: Link Rect: 221, 670, 480, 691 Annotation: Subtype: Link Rect: 221, 645, 466, 666 Annotation: Subtype: Link Rect: 221, 620, 488, 641 Annotation: Subtype: Link Rect: 221, 595, 430, 616 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Annotation: Subtype: Link Rect: 49, 700, 125, 715 Annotation: Subtype: Link Rect: 60, 502, 149, 509 Annotation: Subtype: Link Rect: 49, 290, 159, 297 Annotation: Subtype: Link Rect: 49, 159, 195, 166 Annotation: Subtype: 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