3b56f54c90b1c373354c89146676233d plamolbio_a2024m10v114n5p105.pdf e7700a50a63050f8ea8d4f998f4ea5e1fdbd26cc plamolbio_a2024m10v114n5p105.pdf 0cce923f07d6340acc9d2b95bbae0218d3cdb5c47794b4bcf8d55b2613bfcfb3 plamolbio_a2024m10v114n5p105.pdf Title: Silencing Osa-miR827 via CRISPR/Cas9 protects rice against the blast fungus Magnaporthe oryzae Subject: Plant Molecular Biology, https://doi.org/10.1007/s11103-024-01496-z Keywords: Blast; CRISPR/Cas9; Magnaporthe oryzae; miR827; Phosphate; Oryza sativa Author: Mireia Bundó Creator: Springer Producer: Acrobat Distiller 10.1.8 (Windows) CreationDate: Thu Sep 19 04:30:19 2024 CEST ModDate: Wed Oct 9 09:32:03 2024 CEST Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 790.866 pts Page rot: 0 File size: 2239820 bytes Optimized: yes PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ 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1267 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: Silencing Osa-miR827 via CRISPRCas9 protects rice against the blast fungus Magnaporthe oryzae Children: Item: Title: Abstract Item: Title: Key message Item: Title: Introduction Item: Title: Materials and methods Children: Item: Title: Plant materials, genotyping and growth conditions Item: Title: Design of gRNAs and construction of the CRISPRCas9 vector for MIR827 editing Item: Title: Measurement of phosphate content Item: Title: Blast resistance assays Item: Title: RNA isolation and expression analyses Item: Title: Results Children: Item: Title: MIR827 overexpression enhances susceptibility to infection by the rice blast fungus M. oryzae Item: Title: CRISPRCas9-mediated mutagenesis of MIR827 enhances resistance to infection by M. oryzae Item: Title: miR827 expression is down-regulated during fungal infection Item: Title: Alterations in MIR827 expression result in modified Pi levels Item: Title: Discussion Item: Title: Acknowledgements Item: Title: References Info: Title: Silencing Osa-miR827 via CRISPR/Cas9 protects rice against the blast fungus Magnaporthe oryzae Author: Mireia Bundó Subject: Plant Molecular Biology, https://doi.org/10.1007/s11103-024-01496-z Keywords: Blast; CRISPR/Cas9; Magnaporthe oryzae; miR827; Phosphate; Oryza sativa Creator: Springer Producer: Acrobat Distiller 10.1.8 (Windows) CreationDate: Thu Sep 19 05:00:19 CEST 2024 ModDate: Wed Oct 09 10:02:03 CEST 2024 ID: 0xe1935b89e4d1b74891a3ad72ef5c5437, 0xaa275bcdaa554745898f6cf86a24eada Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: 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