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DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Language: English Outlines: Item: Title: Immunomolecular and reactivity landscapes of gut IgA subclasses in homeostasis and inflammatory bowel disease Destination: titlink1 Children: Item: Title: Introduction Destination: flink1 Item: Title: Results Destination: flink2 Children: Item: Title: IgA2+ PCs emerge along with IgA1+ PCs early after birth and in adults exceed IgA1+ PCs in the distal gut Destination: slink1 Item: Title: Intestinal PCs with terminally differentiated phenotype are enriched in IgA2 expression Destination: slink2 Item: Title: Intestinal IgA1+ and IgA2+ cells are somatically mutated and largely clonally related Destination: slink3 Item: Title: Some intestinal IgA2+ cells may emerge from IgA1+ precursors without accumulating supplemental SHM Destination: slink4 Item: Title: Intestinal SIgA1 and SIgA2 show shared as well as unique reactivity to mucus Destination: slink5 Item: Title: Gut inflammation is associated with the expansion of IgA1+ PBs and depletion of IgA2+ PCs Destination: slink6 Item: Title: Inflammation alters both coating and selection of gut mucus Destination: slink7 Item: Title: Discussion Destination: flink3 Item: Title: Materials and methods Destination: flink4 Children: Item: Title: Human tissue and blood specimens Destination: slink8 Item: Title: Human fecal and mucus samples Destination: slink9 Item: Title: Processing of tissue samples Destination: slink10 Item: Title: Flow cytometry Destination: slink11 Item: Title: Cell sorting Destination: slink12 Item: Title: Immunomagnetic cell separation Destination: slink13 Item: Title: Cell cultures Destination: slink14 Item: Title: IFA Destination: slink15 Item: Title: Sequencing of Ig gene repertoires Destination: slink16 Item: Title: Ig gene repertoire analysis Destination: slink17 Children: Item: Title: Data preprocessing Destination: sslink1 Item: Title: Annotation, clonal assignment, and repertoire analysis Destination: sslink2 Item: Title: Ig gene lineage Destination: sslink3 Item: Title: Cell isolation, single Destination: slink18 Item: Title: Mucus and feces collection and processing Destination: slink19 Item: Title: ELISAs Destination: slink20 Item: Title: Bacterial flow cytometry and sorting Destination: slink21 Item: Title: Bacterial 16S rRNA gene analysis Destination: slink22 Item: Title: Bacterial reactivity of secreted Igs from mucus Destination: slink23 Item: Title: Quantification and statistical analysis Destination: slink24 Item: Title: Online supplemental material Destination: slink25 Item: Title: Acknowledgments Destination: flink5 Item: Title: References Destination: rlink1 Item: Title: Outline placeholder Destination: unknown Children: Item: Title: Supplemental material Destination: flink1 Children: Item: Title: Outline placeholder Destination: unknown Children: Item: Title: Provided online are Table S1, Table S2, Table S3, Table S4, Table S5, Table S6, Table S7, and Table S8. Tables S1 shows tis ... 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