1f5aafdc05f5978976845c5d4dcef24d 324413.pdf 1246b81b666e9dede846a868f9c2b9ecb2215073 324413.pdf 94591a05d871a006613fa67b2c63a00fa0f4b4baca361e592ec7a831925f7f14 324413.pdf Title: Exploring Plasma Coenzyme Q10 Status in Paediatric Dyslipidaemia Subject: Coenzyme Q10 (CoQ) is a ubiquitous lipid with different biological functions. In blood, there is a close relationship between CoQ status and cholesterol, which strongly supports the study of both molecules simultaneously. The objective of this study was to evaluate plasma CoQ, lipoprotein concentrations and CoQ/Chol ratio in a cohort of paediatric patients with different types of dyslipidaemias. A total of 60 paediatric patients were recruited (age range: 7 months–18 years), including 52 with different types of hypercholesterolemia, 2 with isolated hypertriglyceridemia and 6 with hypobetalipoproteinemia. Plasma CoQ was analysed by HPLC with electrochemical detection, and lipoprotein and cholesterol concentrations by standard automated methods. The lowest CoQ values were detected in patients with hypobetalipoproteinemia and in two cases of liver cirrhosis. Mean CoQ values were significantly higher in hypercholesterolemic patients compared to controls (average values 1.07 mol/L and 0.63 mol/L) while the CoQ/cholesterol ratio did not show differences (170 vs. 163, respectively). Mean CoQ values were significantly lower in the group of patients with hypobetalipoproteinemia compared to controls (mean CoQ values of 0.22 mol/L vs. 0.63 mol/L, respectively), while those of CoQ/cholesterol did not show differences. Pearson’s correlation test showed a positive correlation between the CoQ and cholesterol values (r = 0.565, p < 0.001) and between the CoQ and the LDL cholesterol values (r = 0.610, p < 0.001). Our results suggest that it is advisable to analyse plasma CoQ and cholesterol concentrations in patients with hypobetalipoproteinemia and hypercholesterolemia associated with liver damage. Keywords: cholesterol; coenzyme Q10; hypercholesterolemia; hypobetalipoproteinemia; paediatric patients; dyslipidaemia Author: Beatriz Minguez, Mariela de Los Santos, Camila Garcia-Volpe, Cristina Molera, Abraham J. Paredes-Fuentes, Clara Oliva, Angela Arias, Helena Rodriguez-Gonzalez, Delia Yubero, Mireia Tondo, Carlos Santos-Ocaña, Silvia Meavilla and Rafael Artuch Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Aug 9 08:20:00 2024 CEST ModDate: Fri Aug 9 08:23:22 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 703376 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PJPSZX+VnURWPalladioL Type 1 Custom yes yes yes 10 0 CAMXTK+EURM10 Type 1 Builtin yes yes yes 16 0 SOCKQF+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 SVIDVD+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 QAWSNE+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 MBMKLC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 74 0 MBMKLD+PalatinoLinotype CID TrueType Identity-H yes yes yes 77 0 MBMKLE+PalatinoLinotype TrueType WinAnsi yes yes no 83 0 MBMKLG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 86 0 MBMLDE+PalatinoLinotype TrueType MacRoman yes yes no 89 0 XIRVNA+EURB10 Type 1 Builtin yes yes yes 97 0 QMDDXF+CMSY10 Type 1 Builtin yes yes yes 102 0 MBMKLC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 119 0 MBMKLD+PalatinoLinotype CID TrueType Identity-H yes yes yes 122 0 MBMKLE+PalatinoLinotype TrueType WinAnsi yes yes no 128 0 MBMKLG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 131 0 MBMLDE+PalatinoLinotype TrueType MacRoman yes yes no 134 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-01-24 04:21:43 CET RepresentationInformation: 324413.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-01-23 12:52:20 CET Size: 703376 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 281 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Subjects Destination: subsection.2.1 Item: Title: Samples and Laboratory Analysis Destination: subsection.2.2 Item: Title: Statistical Analysis Destination: subsection.2.3 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Exploring Plasma Coenzyme Q10 Status in Paediatric Dyslipidaemia Author: Beatriz Minguez, Mariela de Los Santos, Camila Garcia-Volpe, Cristina Molera, Abraham J. Paredes-Fuentes, Clara Oliva, Angela Arias, Helena Rodriguez-Gonzalez, Delia Yubero, Mireia Tondo, Carlos Santos-Ocaña, Silvia Meavilla and Rafael Artuch Subject: Coenzyme Q10 (CoQ) is a ubiquitous lipid with different biological functions. In blood, there is a close relationship between CoQ status and cholesterol, which strongly supports the study of both molecules simultaneously. The objective of this study was to evaluate plasma CoQ, lipoprotein concentrations and CoQ/Chol ratio in a cohort of paediatric patients with different types of dyslipidaemias. A total of 60 paediatric patients were recruited (age range: 7 months 18 years), including 52 with different types of hypercholesterolemia, 2 with isolated hypertriglyceridemia and 6 with hypobetalipoproteinemia. Plasma CoQ was analysed by HPLC with electrochemical detection, and lipoprotein and cholesterol concentrations by standard automated methods. The lowest CoQ values were detected in patients with hypobetalipoproteinemia and in two cases of liver cirrhosis. Mean CoQ values were significantly higher in hypercholesterolemic patients compared to controls (average values 1.07 mol/L and 0.63 mol/L) while the CoQ/cholesterol ratio did not show differences (170 vs. 163, respectively). Mean CoQ values were significantly lower in the group of patients with hypobetalipoproteinemia compared to controls (mean CoQ values of 0.22 mol/L vs. 0.63 mol/L, respectively), while those of CoQ/cholesterol did not show differences. Pearson’s correlation test showed a positive correlation between the CoQ and cholesterol values (r = 0.565, p < 0.001) and between the CoQ and the LDL cholesterol values (r = 0.610, p < 0.001). Our results suggest that it is advisable to analyse plasma CoQ and cholesterol concentrations in patients with hypobetalipoproteinemia and hypercholesterolemia associated with liver damage. Keywords: cholesterol; coenzyme Q10; hypercholesterolemia; hypobetalipoproteinemia; paediatric patients; dyslipidaemia Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Aug 09 08:20:00 CEST 2024 ModDate: Fri Aug 09 08:23:22 CEST 2024 ID: 0x0727658f5695369d9a3558dce45eddc5, 0x0727658f5695369d9a3558dce45eddc5 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QAWSNE+URWPalladioL-Ital FontSubset: true FirstChar: 46 LastChar: 122 FontDescriptor: FontName: QAWSNE+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: CAMXTK+EURM10 FontSubset: true FirstChar: 12 LastChar: 22 FontDescriptor: FontName: CAMXTK+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true ToUnicode: true Font: BaseFont: XIRVNA+EURB10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: XIRVNA+EURB10 Flags: Symbolic FontBBox: -30, -247, 1178, 723 FontFile: 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