89c3ef7e5c781d40c22ebd9462b29cec 324419.pdf 02a1676bace33528cef6ee178982ab81a4229dfd 324419.pdf cb576004e868b7e4bd646cd8191a8615d3f5ad8517f7fd17a3fa640753fde20a 324419.pdf Title: Lymphatic Regeneration after Popliteal Lymph Node Excision and Implantation of Aligned Nanofibrillar Collagen Scaffolds: An Experimental Rabbit Model Subject: Lymphedema presents significant challenges to patients’ quality of life, prompting the exploration of innovative treatments, such as collagen scaffolds, aimed at treating and reducing the risk of lymphedema. We aimed to evaluate the preventive and therapeutic efficacy and the lymphangiogenic potential of implanted aligned nanofibrillar collagen scaffolds (BioBridgeTM) following the induction of secondary lymphedema in a rabbit model. Thirty rabbits were divided into treatment (G1), prevention (G2), and control (G3) groups. Secondary lymphedema was induced in all groups. BioBridgeTM implantation was performed in G2 and G1 on days 0 and 60, respectively. Follow-ups included hindlimb circumference measurements and indocyanine green lymphography at 0, 60, and 90 days. None of the study rabbits exhibited dermal backflow on day 0 before surgery. At 60 days, the incidence rates of dermal backflow in G1, G2, and G3 were 100%, 44.4%, and 90%, respectively. Furthermore, at 90 days, the incidence rates were 22.2%, 44.4%, and 90%, respectively. New linear lymphatic observation was seen in rabbits with resolved dermal backflow. The findings of this study demonstrated the capacity of BioBridgeTM scaffolds to induce new lymphatic vessel formation and reduce dermal backflow in secondary lymphedema in a rabbit model. Keywords: BioBridge; lymphedema; microsurgery; rabbit animal model; lymphatic regeneration Author: José Luis Campos, Gemma Pons, Ali M. Al-Sakkaf, Irene Laura Lusetti, Laura Pires, Francisco Javier Vela, Elena Ramos, Verónica Crisóstomo, Francisco Miguel Sánchez-Margallo, Elena Abellán and Jaume Masiá Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Thu Aug 22 04:41:20 2024 CEST ModDate: Thu Aug 22 06:05:20 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5557851 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 WMETBB+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 ICZHMV+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 ACUMKR+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 ASAAXQ+VnURWPalladioL Type 1 Custom yes yes yes 32 0 OLCSDB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 51 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 77 0 LGTZLE+CMSY10 Type 1 Builtin yes yes yes 90 0 IJKIGF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 124 0 IJKIGG+PalatinoLinotype CID TrueType Identity-H yes yes yes 127 0 IJKIGH+PalatinoLinotype TrueType WinAnsi yes yes no 133 0 IJKIGJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 136 0 IJKIOI+PalatinoLinotype TrueType MacRoman yes yes no 139 0 IJKIGF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 149 0 IJKIGG+PalatinoLinotype CID TrueType Identity-H yes yes yes 152 0 IJKIGH+PalatinoLinotype TrueType WinAnsi yes yes no 158 0 IJKIGJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 161 0 IJKIOI+PalatinoLinotype TrueType MacRoman yes yes no 164 0 IJKIGF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 177 0 IJKIGG+PalatinoLinotype CID TrueType Identity-H yes yes yes 180 0 IJKIGH+PalatinoLinotype TrueType WinAnsi yes yes no 186 0 IJKIGJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 189 0 IJKIOI+PalatinoLinotype TrueType MacRoman yes yes no 192 0 IJKIGF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 204 0 IJKIGG+PalatinoLinotype CID TrueType Identity-H yes yes yes 207 0 IJKIGH+PalatinoLinotype TrueType WinAnsi yes yes no 213 0 IJKIGJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 216 0 IJKIOI+PalatinoLinotype TrueType MacRoman yes yes no 219 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-01-17 04:31:58 CET RepresentationInformation: 324419.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-01-16 13:04:53 CET Size: 5557851 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 386 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Pre-Operative Procedures Destination: subsection.2.1 Item: Title: Intra-Operative Procedures Destination: subsection.2.2 Item: Title: Postoperative Procedures Destination: subsection.2.3 Item: Title: Histopathological and Immunohistochemical Analyses Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Treatment Group (G1) Destination: subsection.3.1 Item: Title: Preventive Group (G2) Destination: subsection.3.2 Item: Title: Control Group (G3) Destination: subsection.3.3 Item: Title: Follow-Up Assessments Destination: subsection.3.4 Item: Title: Histological Analysis Destination: subsection.3.5 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Lymphatic Regeneration after Popliteal Lymph Node Excision and Implantation of Aligned Nanofibrillar Collagen Scaffolds: An Experimental Rabbit Model Author: José Luis Campos, Gemma Pons, Ali M. Al-Sakkaf, Irene Laura Lusetti, Laura Pires, Francisco Javier Vela, Elena Ramos, Verónica Crisóstomo, Francisco Miguel Sánchez-Margallo, Elena Abellán and Jaume Masiá Subject: Lymphedema presents significant challenges to patients’ quality of life, prompting the exploration of innovative treatments, such as collagen scaffolds, aimed at treating and reducing the risk of lymphedema. We aimed to evaluate the preventive and therapeutic efficacy and the lymphangiogenic potential of implanted aligned nanofibrillar collagen scaffolds (BioBridgeTM) following the induction of secondary lymphedema in a rabbit model. Thirty rabbits were divided into treatment (G1), prevention (G2), and control (G3) groups. Secondary lymphedema was induced in all groups. BioBridgeTM implantation was performed in G2 and G1 on days 0 and 60, respectively. Follow-ups included hindlimb circumference measurements and indocyanine green lymphography at 0, 60, and 90 days. None of the study rabbits exhibited dermal backflow on day 0 before surgery. At 60 days, the incidence rates of dermal backflow in G1, G2, and G3 were 100%, 44.4%, and 90%, respectively. Furthermore, at 90 days, the incidence rates were 22.2%, 44.4%, and 90%, respectively. New linear lymphatic observation was seen in rabbits with resolved dermal backflow. The findings of this study demonstrated the capacity of BioBridgeTM scaffolds to induce new lymphatic vessel formation and reduce dermal backflow in secondary lymphedema in a rabbit model. 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