adb2f82e580bf17ffd825ae5fd4a847c 49DV_Cruz-Sanchez_et_al_2024_membranes.pdf 0b0166a89ec40ce7ec4cf78461bed6cdb7d2b1da 49DV_Cruz-Sanchez_et_al_2024_membranes.pdf e27bc59c4dcd582267d6a7bd6654bb6cb50dce2c11f8d3739006db94a483af3a 49DV_Cruz-Sanchez_et_al_2024_membranes.pdf Title: Antibacterial Activity Assessment of Chitosan/Alginate Lavender Essential Oil Membranes for Biomedical Applications Subject: The demand for natural products in the treatment of dermatological pathologies has boosted the use of bioactive substances such as lavender essential oil (LEO), which stands out for its anti-inflammatory and antioxidant properties and its antimicrobial potential. Biopolymers such as chitosan (CHT) and alginate (ALG) are biodegradable and biocompatible and have proven their viability in biomedical applications such as skin regeneration. The inhibitory effect of LEO on the growth of skin-related bacterial species Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and the fungus Candida albicans was studied by incorporating 1% v/v LEO encapsulated in CHT, ALG, and CHT/ALG membranes. Despite the verification of the antimicrobial effect of all type of membranes, no synergistic effect was observed following the addition of LEO. S. aureus and P. aeruginosa showed the most growth on the different substrates and C. albicans demonstrated the highest inhibition. This is a first approach using microorganisms isolated from clinical samples or skin microbiota. Further investigation would be advisable using more clinical strains for each microorganism to validate their biomedical applicability. Keywords: lavender essential oil; antimicrobial activity; chitosan/alginate membranes Author: Encarnación Cruz Sánchez, María Teresa García, Ignacio Gracia, Soledad Illescas Fernández-Bermejo, Juan Francisco Rodríguez, Jesús Manuel García-Vargas and Dolors Vidal Roig Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Jan 1 08:40:02 2024 CET ModDate: Mon Jan 1 08:46:33 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3016369 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JCYIDT+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 PJKXUD+VnURWPalladioL Type 1 Custom yes yes yes 16 0 XFLVHG+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 MBVFOL+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 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OOLFNC+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 444 0 OOLGGA+PalatinoLinotype TrueType MacRoman yes yes no 447 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-07-16 04:05:23 CEST RepresentationInformation: 49DV_Cruz-Sanchez_et_al_2024_membranes.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-07-15 07:01:33 CEST Size: 3016369 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 654 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Preparation of Membranes Destination: subsection.2.1 Children: Item: Title: Materials Destination: subsubsection.2.1.1 Item: Title: Membranes Synthesis Procedure Destination: subsubsection.2.1.2 Item: Title: Sterilization of Membranes Destination: subsubsection.2.1.3 Item: Title: Antimicrobial Capacity Analysis Destination: subsection.2.2 Children: Item: Title: Strains and Culture Media Destination: subsubsection.2.2.1 Item: Title: Inoculum Solutions Destination: subsubsection.2.2.2 Item: Title: Determination of Bactericidal Effect of LEO Destination: subsubsection.2.2.3 Item: Title: Microbiological Study of Direct-Contact Membranes in Solid Media Destination: subsubsection.2.2.4 Item: Title: Microbiological Study of Membranes in Broth Destination: subsubsection.2.2.5 Item: Title: Database and Statistical Analysis Destination: subsubsection.2.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: Bactericidal Effect of LEO Destination: subsection.3.1 Item: Title: Growth Inhibition Depending on the Time between Inoculum Addition and Culture Destination: subsection.3.2 Item: Title: Bacterial Growth Resulting from Membranes Cultured Directly on Plates Destination: subsection.3.3 Item: Title: Bacterial Growth Resulting from Membranes Cultured in Broth Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Antibacterial Activity Assessment of Chitosan/Alginate Lavender Essential Oil Membranes for Biomedical Applications Author: Encarnación Cruz Sánchez, María Teresa García, Ignacio Gracia, Soledad Illescas Fernández-Bermejo, Juan Francisco Rodríguez, Jesús Manuel García-Vargas and Dolors Vidal Roig Subject: The demand for natural products in the treatment of dermatological pathologies has boosted the use of bioactive substances such as lavender essential oil (LEO), which stands out for its anti-inflammatory and antioxidant properties and its antimicrobial potential. Biopolymers such as chitosan (CHT) and alginate (ALG) are biodegradable and biocompatible and have proven their viability in biomedical applications such as skin regeneration. The inhibitory effect of LEO on the growth of skin-related bacterial species Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and the fungus Candida albicans was studied by incorporating 1% v/v LEO encapsulated in CHT, ALG, and CHT/ALG membranes. Despite the verification of the antimicrobial effect of all type of membranes, no synergistic effect was observed following the addition of LEO. S. aureus and P. aeruginosa showed the most growth on the different substrates and C. albicans demonstrated the highest inhibition. This is a first approach using microorganisms isolated from clinical samples or skin microbiota. Further investigation would be advisable using more clinical strains for each microorganism to validate their biomedical applicability. 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