d55eb79e83aef5e394e7145366638364 IJMS_a2024v25p04474.pdf ceb5655006746da8669a5725d29c376899a7e92b IJMS_a2024v25p04474.pdf 9fee5da9739efb30c0f807b15e5bc2b6fe233765d6e4de18dd10eda4c2b7942e IJMS_a2024v25p04474.pdf Title: Oocyte Competence of Prepubertal Sheep and Goat Oocytes:An Assessment of Large-Scale Chromatin Configuration and Epidermal Growth Factor Receptor Expression in Oocytes and Cumulus Cells Subject: The oocyte competence of prepubertal females is lower compared to that of adults, mainly because they originate from small follicles. In adult females, the germinal vesicle (GV) and epidermal growth factor receptor (EGFR) have been associated with oocyte competence. This study aimed to analyze GV chromatin configuration and EGFR expression in prepubertal goat and sheep oocytes obtained from small (<3 mm) and large (3 mm) follicles and compare them with those from adults. GV chromatin was classified from diffuse to condensed as GV1, GVn, and GVc for goats and NSN, SN, and SNE for sheep. EGFR was quantified in cumulus cells (CCs) by Western blotting and in oocytes by immunofluorescence. Oocytes from prepubertal large follicles and adults exhibited highly condensed chromatin in goats (71% and 69% in GVc, respectively) and sheep (59% and 75% in SNE, respectively). In both species, EGFR expression in CCs and oocytes was higher in prepubertal large follicles than in small ones. In adult females, EGFR expression in oocytes was higher than in prepubertal large follicles. In conclusion, GV configuration and EGFR expression in CCs and oocytes were higher in the large than small follicles of prepubertal females. Keywords: oocyte; goat; sheep; germinal vesicle; EGF receptor Author: Mònica Ferrer-Roda, Dolors Izquierdo, Ana Gil, Maria Emilia Franco Oliveira and Maria-Teresa Paramio Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Apr 19 06:52:37 2024 CEST ModDate: Fri Apr 19 06:55:40 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3125187 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WLFTRW+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 XIQPOK+VnURWPalladioL Type 1 Custom yes yes yes 16 0 SMFOLC+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 LSAVKF+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 SRKTTY+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 WHUIJM+CMSY10 Type 1 Builtin yes yes yes 37 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 53 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 56 0 ZXDKIP+EURM10 Type 1 Builtin yes yes yes 76 0 DOFJKM+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 84 0 DOFJKN+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 90 0 DOFJPM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 96 0 DOFJPN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 102 0 DOFJPO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 105 0 DOFJPP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 108 0 DOFJKM+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 119 0 DOFJKN+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 125 0 DOFJPM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 131 0 DOFJPN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 137 0 DOFJPO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 140 0 DOFJPP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 143 0 DOFLMF+PalatinoLinotype-BoldItalic CID TrueType Identity-H yes yes yes 146 0 DOFJKM+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 162 0 DOFJKN+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 168 0 DOFJPM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 174 0 DOFJPN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 180 0 DOFJPO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 183 0 DOFJPP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 186 0 DOFJKM+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 204 0 DOFJKN+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 210 0 DOFJPM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 216 0 DOFJPN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 222 0 DOFJPO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 225 0 DOFJPP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 228 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-08 02:43:11 CEST RepresentationInformation: IJMS_a2024v25p04474.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-05-07 05:55:15 CEST Size: 3125187 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 353 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Germinal Vesicle Chromatin Configuration Destination: subsection.2.1 Item: Title: EGFR Expression in Cumulus Cells Destination: subsection.2.2 Item: Title: EGFR Expression in Oocytes Destination: subsection.2.3 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Oocyte Collection Destination: subsection.4.1 Item: Title: Assessment of Oocyte Germinal Vesicle Chromatin Configurations Destination: subsection.4.2 Item: Title: Epidermal Growth Factor Receptor (EGFR) Quantification Destination: subsection.4.3 Children: Item: Title: Western Blotting Destination: subsubsection.4.3.1 Item: Title: Immunofluorescence Destination: subsubsection.4.3.2 Item: Title: Statistical Analyses Destination: subsection.4.4 Item: Title: References Destination: section.5 Info: Title: Oocyte Competence of Prepubertal Sheep and Goat Oocytes:An Assessment of Large-Scale Chromatin Configuration and Epidermal Growth Factor Receptor Expression in Oocytes and Cumulus Cells Author: Mònica Ferrer-Roda, Dolors Izquierdo, Ana Gil, Maria Emilia Franco Oliveira and Maria-Teresa Paramio Subject: The oocyte competence of prepubertal females is lower compared to that of adults, mainly because they originate from small follicles. In adult females, the germinal vesicle (GV) and epidermal growth factor receptor (EGFR) have been associated with oocyte competence. This study aimed to analyze GV chromatin configuration and EGFR expression in prepubertal goat and sheep oocytes obtained from small (<3 mm) and large (3 mm) follicles and compare them with those from adults. GV chromatin was classified from diffuse to condensed as GV1, GVn, and GVc for goats and NSN, SN, and SNE for sheep. EGFR was quantified in cumulus cells (CCs) by Western blotting and in oocytes by immunofluorescence. Oocytes from prepubertal large follicles and adults exhibited highly condensed chromatin in goats (71% and 69% in GVc, respectively) and sheep (59% and 75% in SNE, respectively). In both species, EGFR expression in CCs and oocytes was higher in prepubertal large follicles than in small ones. In adult females, EGFR expression in oocytes was higher than in prepubertal large follicles. In conclusion, GV configuration and EGFR expression in CCs and oocytes were higher in the large than small follicles of prepubertal females. 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