4c7b5b24a4fef59b2e5fe8132090c415 ANIM_a2024v14p3362.pdf 892ad20cd1bb98731672dd1103575fa5b7a0c642 ANIM_a2024v14p3362.pdf 0cb12bac27a767cacdd8932012d7aabed2c963c44d30122fa760330bff7306a9 ANIM_a2024v14p3362.pdf Title: Characterization of Feline Basophils on the Sysmex XN-1000V and Evaluation of a New WDF Gating Profile Subject: The Sysmex XN-1000V provides a percentage and concentration of basophils from the WNR scattergram, as for human samples, but this method has been shown to be irrelevant in cats. This study aimed to characterize the feline basophil distribution on the WDF channel and to preliminarily evaluate the performance of a new basophil gate on the WDF channel. Cases of feline basophilia were retrospectively retrieved and the scattergram from the WDF and WNR channels were evaluated for any consistent pattern. A new gating setting for the WDF channel was created to include the suspected basophil region. This new gating was applied retrospectively to identified basophilia cases and prospectively to randomly selected feline cases. Manual, WNR, and new WDF methods for basophil identification were compared. Nine cases of feline basophilia were identified. A characteristic WDF scattergram was identified in seven of the nine cases. A new gate was created on the WDF channel and applied to these and 34 additional cases. The comparison study showed that the new method of basophil quantification using the WDF scattergram correlated better with the manual method than the Sysmex XN-1000V method using the WNR scattergram. Basophil concentration in feline peripheral blood can be determined using a new gate on the WDF channel of the Sysmex XN-1000V, which provides better performance than the WNR channel and is comparable to the manual method. Keywords: basophil; cat; eosinophil; hematology analyzer; granulocyte; method comparison Author: Javier Martínez-Caro and Josep Pastor Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Nov 25 09:51:52 2024 CET ModDate: Mon Nov 25 09:56:59 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3664638 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 OHPBRJ+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 RKJEFZ+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 XUDILV+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 VCVFNL+VnURWPalladioL Type 1 Custom yes yes yes 32 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 72 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 75 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 81 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 84 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 87 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 101 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 104 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 110 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 113 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 116 0 ZXDKIP+EURM10 Type 1 Builtin yes yes yes 123 0 SOSCBV+CMSY10 Type 1 Builtin yes yes yes 128 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 140 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 143 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 149 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 152 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 155 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 177 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 180 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 186 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 189 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 192 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 222 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 225 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 231 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 234 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 237 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 264 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 267 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 273 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 276 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 279 0 NLABMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 305 0 NLABMB+PalatinoLinotype CID TrueType Identity-H yes yes yes 308 0 NLABMC+PalatinoLinotype TrueType WinAnsi yes yes no 314 0 NLABME+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 317 0 NLACAE+PalatinoLinotype TrueType MacRoman yes yes no 320 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-01-30 02:45:29 CET RepresentationInformation: ANIM_a2024v14p3362.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-01-29 08:32:01 CET Size: 3664638 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 468 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Preliminary Phase Destination: subsection.2.1 Item: Title: Comparison Study Destination: subsection.2.2 Item: Title: Results Destination: section.3 Children: Item: Title: Preliminary Phase Destination: subsection.3.1 Item: Title: Comparison Study Destination: subsection.3.2 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Characterization of Feline Basophils on the Sysmex XN-1000V and Evaluation of a New WDF Gating Profile Author: Javier Martínez-Caro and Josep Pastor Subject: The Sysmex XN-1000V provides a percentage and concentration of basophils from the WNR scattergram, as for human samples, but this method has been shown to be irrelevant in cats. This study aimed to characterize the feline basophil distribution on the WDF channel and to preliminarily evaluate the performance of a new basophil gate on the WDF channel. Cases of feline basophilia were retrospectively retrieved and the scattergram from the WDF and WNR channels were evaluated for any consistent pattern. A new gating setting for the WDF channel was created to include the suspected basophil region. This new gating was applied retrospectively to identified basophilia cases and prospectively to randomly selected feline cases. Manual, WNR, and new WDF methods for basophil identification were compared. Nine cases of feline basophilia were identified. A characteristic WDF scattergram was identified in seven of the nine cases. A new gate was created on the WDF channel and applied to these and 34 additional cases. The comparison study showed that the new method of basophil quantification using the WDF scattergram correlated better with the manual method than the Sysmex XN-1000V method using the WNR scattergram. Basophil concentration in feline peripheral blood can be determined using a new gate on the WDF channel of the Sysmex XN-1000V, which provides better performance than the WNR channel and is comparable to the manual method. 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