d37ebf662a3771f648a235c4fb9bb7eb agriculture-14-02184-v2.pdf 24bd6c024e39b42a9294200ef98d7228a605b066 agriculture-14-02184-v2.pdf 1b400bdb386dfa13e5cb272dac6b6a3a1b41835e3e2b01cce10aa0efc56bed27 agriculture-14-02184-v2.pdf Title: Seed Inoculation with Halotolerant Strains Enhance Brassicaceae Seedling Establishment Under Saline Conditions Subject: Soil salinity inhibits germination and seedling establishment, causing patchy crop stands, uneven growth, and poor yields. This study aims to evaluate the early-stage salinity tolerance of Brassicaceae seeds inoculated with plant growth-promoting bacterial (PGPB) strains (E1 and T7) isolated from saline soils. Non-inoculated and inoculated seeds of Lobularia maritima, Sinapis alba, and Brassica napus were cultivated under control and salinity conditions, first in agar plates to assess a germination inhibitory concentration of salt for each species and later in soil irrigated with water containing 0 or 75 mM NaCl. Our results indicate that T7 was the only strain able to increase the germination of L. maritima under saline conditions. However, an increase in shoot biomass, root length, and number of branches was observed in L. maritima and S. alba plants inoculated with T7 and in B. napus with E1. Concomitantly, those seedlings exhibited less oxidative damage and greater capacity to balance plant reactive oxygen species production. This study suggests that inoculation of seeds with halotolerant PGPB strains is a suitable strategy for improving the negative effects of salinity in the early stages. Nonetheless, the observed specific plant–host interaction highlights the need for establishing tailored PGPB–crop associations for specific unfavourable environmental conditions. Keywords: salinity; PGPB; Brassicaceae; seed inoculation; seedling establishment Author: Carlos González-Cobo, Glòria Escolà, Roser Tolrà, Mercè Llugany, Charlotte Poschenrieder, Eliana Bianucci and Silvia Busoms Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Dec 9 08:26:55 2024 CET ModDate: Mon Dec 9 10:24:37 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 18 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2836470 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UBXXGV+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 OBWWPJ+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 MALIMA+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 ZUSPOE+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 OJYQXC+CMSY10 Type 1 Builtin yes yes yes 32 0 EKRHBM+VnURWPalladioL Type 1 Custom yes yes yes 37 0 SPXLNH+PazoMath Type 1 Builtin yes yes yes 70 0 JHYTSG+CMR10 Type 1 Builtin yes yes yes 78 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 83 0 HDPBDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 104 0 HDPBDI+PalatinoLinotype TrueType WinAnsi yes yes no 107 0 HDPBCH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 110 0 GIGOEX+URWPalladioL-BoldItal Type 1 Custom yes yes yes 119 0 HDPBDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 140 0 HDPBDI+PalatinoLinotype TrueType WinAnsi yes yes no 143 0 HDPBCH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 146 0 HDPDAD+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 149 0 HDPBDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 173 0 HDPBDI+PalatinoLinotype TrueType WinAnsi yes yes no 176 0 HDPBCH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 179 0 HDPBDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 206 0 HDPBDI+PalatinoLinotype TrueType WinAnsi yes yes no 209 0 HDPBCH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 212 0 HDPBDH+PalatinoLinotype CID TrueType Identity-H yes yes yes 233 0 HDPBDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 239 0 HDPBDI+PalatinoLinotype TrueType WinAnsi yes yes no 242 0 HDPBCH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 245 0 HDPBDK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 264 0 HDPBDI+PalatinoLinotype TrueType WinAnsi yes yes no 267 0 HDPBCH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 270 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-12 02:42:44 CET RepresentationInformation: agriculture-14-02184-v2.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-11 07:42:14 CET Size: 2836470 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 592 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Material and Methods Destination: section.2 Children: Item: Title: Plant Material and Growth Conditions Destination: subsection.2.1 Item: Title: Bacterial Strains Destination: subsection.2.2 Item: Title: Salt Screening Germination Assay Destination: subsection.2.3 Item: Title: In Vitro Germination Assay Destination: subsection.2.4 Item: Title: Soil Germination Assay Destination: subsection.2.5 Item: Title: Leaf Na+ and K+ Determination Destination: subsection.2.6 Item: Title: ROS Production Destination: subsection.2.7 Item: Title: Root Viability Assay Destination: subsection.2.8 Item: Title: Data Analysis Destination: subsection.2.9 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Salt Screening Germination Assay and Effects of PGPB on Germination Destination: subsection.3.1 Item: Title: Seedling Establishment of Brassica Seeds Inoculated with PGPB Under Saline Soil Conditions Destination: subsection.3.2 Item: Title: Seedling Growth and Root Architecture of Brassicaceae Species Inoculated with PGPB Under Saline Soil Conditions Destination: subsection.3.3 Item: Title: Detection of Oxidative Damage in Brassica Seedlings Inoculated with PGPB Under Salt Conditions Destination: subsection.3.4 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: appendix.A. Info: Title: Seed Inoculation with Halotolerant Strains Enhance Brassicaceae Seedling Establishment Under Saline Conditions Author: Carlos González-Cobo, Glòria Escolà, Roser Tolrà, Mercè Llugany, Charlotte Poschenrieder, Eliana Bianucci and Silvia Busoms Subject: Soil salinity inhibits germination and seedling establishment, causing patchy crop stands, uneven growth, and poor yields. This study aims to evaluate the early-stage salinity tolerance of Brassicaceae seeds inoculated with plant growth-promoting bacterial (PGPB) strains (E1 and T7) isolated from saline soils. Non-inoculated and inoculated seeds of Lobularia maritima, Sinapis alba, and Brassica napus were cultivated under control and salinity conditions, first in agar plates to assess a germination inhibitory concentration of salt for each species and later in soil irrigated with water containing 0 or 75 mM NaCl. Our results indicate that T7 was the only strain able to increase the germination of L. maritima under saline conditions. However, an increase in shoot biomass, root length, and number of branches was observed in L. maritima and S. alba plants inoculated with T7 and in B. napus with E1. Concomitantly, those seedlings exhibited less oxidative damage and greater capacity to balance plant reactive oxygen species production. This study suggests that inoculation of seeds with halotolerant PGPB strains is a suitable strategy for improving the negative effects of salinity in the early stages. Nonetheless, the observed specific plant host interaction highlights the need for establishing tailored PGPB crop associations for specific unfavourable environmental conditions. 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