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Title:           Blastocoel fluid aspiration improves vitrification outcomes and produces similar sexing results of in vitro -produced cattle embryos compared to microblade biopsy
Subject:         Theriogenology, 218 (2024) 142-152. doi:10.1016/j.theriogenology.2024.01.042
Keywords:        Cell-free DNA,Blastocoel collapse,Blastocoel fluid,Trophectoderm biopsy,Microblade,ICSI pipette,BRY4a,SAT1,PCR,RT-qPCR
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     Item: 
      Title: Blastocoel fluid aspiration improves vitrification outcomes and produces similar sexing results of in vitro-produced cattle ...
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 Chemicals and suppliers
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          Title: 2.2 Experimental design
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          Title: 2.3 In vitro production of bovine embryos
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          Title: 2.4 Obtaining blastocyst samples for sex determination
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            Title: 2.4.1 BF aspiration and collection by blastocoel cavity collapse
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            Title: 2.4.2 TE conventional biopsy by microblade cutting
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          Title: 2.5 Embryo vitrification and warming
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            Title: 2.5.1 Vitrification protocol
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            Title: 2.5.2 Warming protocol
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          Title: 2.6 Embryo sexing by PCR
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          Title: 2.7 Gene expression analysis
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          Title: 3.1 BF as a source of cfDNA produces sexing results comparable to microblade TE biopsy
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          Title: 3.2 Blastocyst artificial collapse before vitrification improves post-warming re-expansion and hatching rates of D7 IVP embryos
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        Title: Ethics approval and consent to participate
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        Title: Consent for publication
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        Title: Availability of data and materials
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        Title: Funding
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        Title: List of abbreviations
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        Title: Appendix A Supplementary data
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  Checksum: 40bf5aed
   Type: CRC32
  Checksum: 4b7d129542b63f1e18ae9772cdfbc841
   Type: MD5
  Checksum: a92f2cda3ac6a3100984d0a3834aa0ad164868f5
   Type: SHA-1
  Checksum: 7b7650fdbd091c3569a19ef9a15f684e889e493466b76b8caeadb63b7075fd5b
   Type: SHA-256