b5a270cc66930b8262eca39df45aad17 pmc_10812474.pdf 1fbc3b7c170ed697af6749ee742748c2fc2d65f5 pmc_10812474.pdf d07d5fae3b6017acb4b8ec35446cdc2adbc2a9a1e7a1d7e9abdf433e5c841e32 pmc_10812474.pdf Title: The Impact of Fiber Source on Digestive Function, Fecal Microbiota, and Immune Response in Adult Dogs Subject: This study evaluated the impact of different fiber sources on intestinal function, fecal microbiota, and overall health in dogs. Twelve dogs were used in a crossover design, involving three periods of 6 weeks and three diets: a low-fiber diet (CTR), a cereal-fiber and beet-pulp-supplemented diet (BRA), and a fruit-fiber-supplemented diet (FRU). Each period included a digestibility trial and fecal and blood sampling in the last week. Short-chain fatty acids (SCFAs) and microbiota taxonomy (16S rRNA Illumina-MiSeq) and functionality (Shotgun-NovaSeq 6000) were determined in the feces. General biochemistry, complete blood cells, and lymphocyte subsets were also analyzed. The fiber-supplemented diets showed lower digestibility without significant changes in the fecal consistency. The BRA diet showed higher total SCFA concentrations (p = 0.056), with increases in alpha diversity and particular beneficial genera, such as Lachnospira, Bifidobacterium, and Faecalibacterium. The BRA microbiota was also associated with an overabundance of genes related to carbohydrate and amino acid metabolism. The FRU diet had a distinct impact on the microbiota composition and functionality, leading to higher levels of CD8 lymphocytes. These findings emphasize the importance of selecting the right fiber source when formulating dog diets, as it can have a differential impact on gut microbiota and animal health. Keywords: dietary fiber; dog; microbiota; digestibility; cereal; fruit; short-chain fatty acids; lipid profile Author: Miquel Montserrat-Malagarriga, Lorena Castillejos, Anna Salas-Mani, Celina Torre and Susana M. 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2023-05-18) Date: 2024-02-20 02:33:34 CET RepresentationInformation: pmc_10812474.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-02-14 02:28:39 CET Size: 4916851 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 813 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Diets Destination: subsection.2.1 Item: Title: Experimental Design Destination: subsection.2.2 Item: Title: Analytical Methods Destination: subsection.2.3 Item: Title: Calculations Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Fecal Quality Destination: subsection.3.1 Item: Title: Apparent Digestibility of Nutrients and Metabolizable Energy Destination: subsection.3.2 Item: Title: Fecal Short-Chain Fatty Acids Destination: subsection.3.3 Item: Title: Serum Biochemistry and Complete Blood Counts Destination: subsection.3.4 Item: Title: Fecal Microbiota Structure and Biodiversity Destination: subsection.3.5 Item: Title: Discussion Destination: section.4 Children: Item: Title: Effects on Fecal Quality and Digestive Function Destination: subsection.4.1 Item: Title: Animal Metabolism and Immunity Destination: subsection.4.2 Item: Title: Impact on Intestinal Microbiota: Composition and Functional Changes Destination: subsection.4.3 Item: Title: Conclusions Destination: section.5 Item: Title: Appendix A Destination: section.A. Item: Title: References Destination: section.B. Info: Title: The Impact of Fiber Source on Digestive Function, Fecal Microbiota, and Immune Response in Adult Dogs Author: Miquel Montserrat-Malagarriga, Lorena Castillejos, Anna Salas-Mani, Celina Torre and Susana M. Martín-Orúe Subject: This study evaluated the impact of different fiber sources on intestinal function, fecal microbiota, and overall health in dogs. Twelve dogs were used in a crossover design, involving three periods of 6 weeks and three diets: a low-fiber diet (CTR), a cereal-fiber and beet-pulp-supplemented diet (BRA), and a fruit-fiber-supplemented diet (FRU). Each period included a digestibility trial and fecal and blood sampling in the last week. Short-chain fatty acids (SCFAs) and microbiota taxonomy (16S rRNA Illumina-MiSeq) and functionality (Shotgun-NovaSeq 6000) were determined in the feces. General biochemistry, complete blood cells, and lymphocyte subsets were also analyzed. The fiber-supplemented diets showed lower digestibility without significant changes in the fecal consistency. The BRA diet showed higher total SCFA concentrations (p = 0.056), with increases in alpha diversity and particular beneficial genera, such as Lachnospira, Bifidobacterium, and Faecalibacterium. The BRA microbiota was also associated with an overabundance of genes related to carbohydrate and amino acid metabolism. The FRU diet had a distinct impact on the microbiota composition and functionality, leading to higher levels of CD8 lymphocytes. These findings emphasize the importance of selecting the right fiber source when formulating dog diets, as it can have a differential impact on gut microbiota and animal health. 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