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Keywords: blast, defense response, effectors, Magnaporthe oryzae, Oryza sativa, phosphate, reactive oxygen species Author: Héctor Martín-Cardoso CreationDate: Mon Jan 15 07:57:35 2024 CET ModDate: Mon Jan 15 07:57:35 2024 CET Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.389 x 841.663 pts (A4) Page rot: 0 File size: 14851352 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PDLONL+AdvTTd3a5f740 Type 1C WinAnsi yes yes no 699 0 PDLONM+AdvPSA18C Type 1C WinAnsi yes yes no 700 0 PDLONN+AdvTTd3a5f740+01 Type 1C MacRoman yes yes no 701 0 PDLONO+AdvTT9b12cd41 Type 1C WinAnsi yes yes no 702 0 PDLONP+AdvPSA323 Type 1C WinAnsi yes yes no 703 0 PDLOOA+AdvTT9b12cd41+01 Type 1C Custom yes yes no 704 0 PDLOOB+AdvTT9b12cd41+fb Type 1C Custom yes yes yes 705 0 PDLOPB+AdvTT9b12cd41+20 Type 1C Custom yes yes yes 706 0 PDLOPC+AdvTTd168d80a.I Type 1C WinAnsi yes yes no 707 0 PDLPMO+AdvTT2c8ce45a Type 1C WinAnsi yes yes no 724 0 PDMADN+AdvOT1ef757c0 Type 1C WinAnsi yes yes no 438 0 PDMADO+AdvOT1ef757c0+20 Type 1C WinAnsi yes yes no 444 0 PDMADP+AdvOT7d6df7ab.I Type 1C WinAnsi yes yes no 445 0 PDMAEA+AdvOT1ef757c0+fb Type 1C Custom yes yes yes 446 0 PDMADM+AdvTTc8b77491.I Type 1C WinAnsi yes yes no 439 0 PDMAGA+AdvOT3f84ef53 Type 1C WinAnsi yes yes no 452 0 PDMAGB+AdvOTd877c31c Type 1C WinAnsi yes yes no 455 0 PDMAGC+AdvTT749c1f5a.I Type 1C WinAnsi yes yes no 459 0 PDMAIC+AdvOTd877c31c+20 Type 1C Custom yes yes yes 461 0 PDMAJD+AdvOT1ef757c0+22 Type 1C Custom yes yes no 462 0 PDMAJE+AdvTTd3a5f740+fb Type 1C Custom yes yes yes 464 0 PDMCJI+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 470 0 PDMHAL+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 473 0 PDMKJF+AdvTT58d178b9+03 Type 1C Custom yes yes yes 476 0 PDMCJI+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 481 0 PDMHAL+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 482 0 PDOANG+AdvTTd3a5f740+20 Type 1C WinAnsi yes yes no 483 0 PDOANH+AdvOT1ef757c0+01 Type 1C Custom yes yes no 484 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-09-01 08:13:17 CEST RepresentationInformation: pmc_10827867.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-07-12 02:35:29 CEST Size: 14851352 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ErrorMessage: Invalid indirect destination - referenced object 'SF1' cannot be found ID: PDF-HUL-149 ErrorMessage: Invalid indirect destination - referenced object 'SF2' cannot be found ID: PDF-HUL-149 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SF1' cannot be found ID: PDF-HUL-122 Offset: 14816455 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SF2' cannot be found ID: PDF-HUL-122 Offset: 14816455 MIMEtype: application/pdf PDFMetadata: Objects: 795 FreeObjects: 4 IncrementalUpdates: 3 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Phosphate accumulation in rice leaves promotes fungal pathogenicity and represses host immune responses during pathogen infection Destination: bmark_1398974641 Children: Item: Title: 1 Introduction Destination: s1 Item: Title: 2 Materials and methods Destination: s2 Children: Item: Title: 2.1 Plant and fungal material Destination: s2_1 Item: Title: 2.2 Transformation of M. oryzae Destination: s2_2 Item: Title: 2.3 Pi treatment and measurement of Pi content Destination: s2_3 Item: Title: 2.4 Infection assays and live-cell imaging of M. oryzae effectors in planta Destination: s2_4 Item: Title: 2.5 Expression analysis by RT-qPCR Destination: s2_5 Item: Title: 2.6 Histochemical analysis of ROS accumulation Destination: s2_6 Item: Title: 2.7 Lipid peroxidation assay Destination: s2_7 Item: Title: 2.8 In vitro growth of M. oryzae and calcofluor white staining Destination: s2_8 Item: Title: 2.9 Identification of Pi transporters in M. oryzae Destination: s2_9 Item: Title: 3 Results Destination: s3 Children: Item: Title: 3.1 Treatment of rice plants with high Pi fosters invasive growth of M. oryzae Destination: s3_1 Item: Title: 3.2 Phosphate accumulation in rice leaf sheaths modulates the expression of M. oryzae effector genes Destination: s3_2 Item: Title: 3.3 Phosphate content in rice leaf sheaths alters the expression of suppressors of plant cell death and cell death-inducing effector genes Destination: s3_3 Item: Title: 3.4 Cytological response to M. oryzae infection in leaf sheaths of High-Pi rice plants Destination: s3_4 Item: Title: 3.5 Phosphate stimulates the in vitro growth of M. oryzae Destination: s3_5 Item: Title: 3.6 Expression of M. oryzae phosphate transporters in Pi-treated rice plants Destination: s3_6 Item: Title: 4 Discussion Destination: s4 Item: Title: Data availability statement Destination: s5 Item: Title: Author contributions Destination: s6 Item: Title: Funding Destination: bmark_1865464027 Item: Title: Acknowledgments Destination: bmark_-366698314 Item: Title: Conflict of interest Destination: bmark_1089982005 Item: Title: Publisher’s note Destination: bmark_-1256977648 Item: Title: Supplementary material Destination: bmark_632582120 Item: Title: References Destination: bmark_2090415954 Info: Title: Phosphate accumulation in rice leaves promotes fungal pathogenicity and represses host immune responses during pathogen infection Author: Héctor Martín-Cardoso Subject: Rice is one of the most important crops in the world and a staple food for more than half of the world’s population. 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Copyright © 2024 Martín-Cardoso, Bundó, Val-Torregrosa and San Segundo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 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