644072a1d90db4d55c7ecc7dc5a2e941 pmc_10854788.pdf 97229131f9daceb6cfef45e5de0c74ed44183023 pmc_10854788.pdf b56da628b1e2fd8d3cb7bf68c3b82c908e2b863b5bcc55f9600119c8d31129d6 pmc_10854788.pdf Title: Study of Canine Distemper Virus Presence in Catalonia’s Wild Carnivores through H Gene Amplification and Sequencing Subject: Canine distemper virus (CDV) is recognised worldwide as an important pathogen in both domestic and wild carnivores. Few data are available on its impact and spread on the wildlife/wildlife–domestic animal–environment interface. This study, aimed at developing a conservation-oriented control strategy, analysed 89 sick or deceased animals from 2019 to 2023 at the Wildlife Rehabilitation Centre in Torreferrussa. RT-PCR and sequencing of the partial H gene were used to detect and analyse CDV in tissues. The total positive percentage was 20.22% (18/89), comprising 13 red foxes (44.8%), 4 European badgers (28.6%), and 1 American mink (4.5%), while 24 Eurasian otters tested negative. Phylogenetic analysis indicated that all of the CDV strains belong to the European lineage. Geographically distant individuals and different species shared the same viral strain, suggesting a strong capacity of CDV for interspecies and long-distance transmission. This calls for further research, particularly focusing on potential impacts of CDV on endangered carnivores. Keywords: red fox (Vulpes vulpes); badger (Meles meles); American mink (Neogale vison); Eurasian otter (Lutra lutra); canine distemper virus (CDV) Author: Junhao Huang, Martí Cortey, Laila Darwich, Jenna Griffin, Elena Obón, Rafael Molina and Margarita Martín Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Jan 29 09:47:06 2024 CET ModDate: Mon Jan 29 09:57:13 2024 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3231472 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 CYVJLM+VnURWPalladioL Type 1 Custom yes yes yes 16 0 LCZMMB+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 QESCDB+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 AFBHJJ+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 VIWIOF+CMSY10 Type 1 Builtin yes yes yes 68 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 76 0 IFPAAI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 94 0 IFPAAK+PalatinoLinotype TrueType WinAnsi yes yes no 97 0 IFPAAM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 100 0 IFPAJL+PalatinoLinotype TrueType MacRoman yes yes no 103 0 IFPAAI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 127 0 IFPAAK+PalatinoLinotype TrueType WinAnsi yes yes no 130 0 IFPAAM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 133 0 IFPAJL+PalatinoLinotype TrueType MacRoman yes yes no 136 0 IFPAAI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 151 0 IFPAAJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 154 0 IFPAAK+PalatinoLinotype TrueType WinAnsi yes yes no 160 0 IFPAAM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 163 0 IFPAJL+PalatinoLinotype TrueType MacRoman yes yes no 166 0 NJWNDJ+VnURWPalladioL-Italic Type 1 Custom yes yes yes 186 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-03-07 02:35:31 CET RepresentationInformation: pmc_10854788.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-03-05 02:33:43 CET Size: 3231472 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 440 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Area and Animal Tissues Destination: subsection.2.1 Item: Title: Nucleic Acid Detection and Analysis Destination: subsection.2.2 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Study of Canine Distemper Virus Presence in Catalonia’s Wild Carnivores through H Gene Amplification and Sequencing Author: Junhao Huang, Martí Cortey, Laila Darwich, Jenna Griffin, Elena Obón, Rafael Molina and Margarita Martín Subject: Canine distemper virus (CDV) is recognised worldwide as an important pathogen in both domestic and wild carnivores. Few data are available on its impact and spread on the wildlife/wildlife domestic animal environment interface. This study, aimed at developing a conservation-oriented control strategy, analysed 89 sick or deceased animals from 2019 to 2023 at the Wildlife Rehabilitation Centre in Torreferrussa. RT-PCR and sequencing of the partial H gene were used to detect and analyse CDV in tissues. The total positive percentage was 20.22% (18/89), comprising 13 red foxes (44.8%), 4 European badgers (28.6%), and 1 American mink (4.5%), while 24 Eurasian otters tested negative. Phylogenetic analysis indicated that all of the CDV strains belong to the European lineage. Geographically distant individuals and different species shared the same viral strain, suggesting a strong capacity of CDV for interspecies and long-distance transmission. This calls for further research, particularly focusing on potential impacts of CDV on endangered carnivores. Keywords: red fox (Vulpes vulpes); badger (Meles meles); American mink (Neogale vison); Eurasian otter (Lutra lutra); canine distemper virus (CDV) Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Jan 29 09:47:06 CET 2024 ModDate: Mon Jan 29 09:57:13 CET 2024 ID: 0x0e2781cba131ed0f3472dd14256b0cf1, 0x0e2781cba131ed0f3472dd14256b0cf1 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: CYVJLM+VnURWPalladioL FontSubset: true FirstChar: 129 LastChar: 226 FontDescriptor: FontName: CYVJLM+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: AFBHJJ+URWPalladioL-Ital FontSubset: true FirstChar: 39 LastChar: 241 FontDescriptor: FontName: AFBHJJ+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: VIWIOF+CMSY10 FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: 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