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Title: ProTInSeq: transposon insertion tracking by ultra-deep DNA sequencing to identify translated large and small’ORFs Destination: title_link Children: Item: Title: Results Destination: A1 Children: Item: Title: Mini-transposon engineering to obtain the ProTInSeq library Destination: B1 Item: Title: A sequencing approach to explore protein translation in bacteria Destination: B2 Item: Title: Benchmark of ProTInSeq and comparative with other experimental methods in identifying translation of annotated proteins Destination: B3 Item: Title: Factors affecting protein identification by ProTInSeq and its use for relative quantification of expressed proteins Destination: B4 Item: Title: ProTInSeq insertion profiles represented transmembrane segments for NE proteins Destination: B5 Item: Title: Identification of unannotated proteins and SEPs using ProTInSeq Destination: B6 Item: Title: Function exploration and validation of unannotated SEPs by computational and experimental analyses Destination: B7 Item: Title: Discussion Destination: A2 Item: Title: Methods Destination: A3 Children: Item: Title: Molecular cloning Destination: B8 Item: Title: Bacterial strains and growth conditions Destination: B9 Item: Title: Transformation of M. pneumoniae Destination: B10 Item: Title: Transformation of mycoplasma agalactiae Destination: B11 Item: Title: Estimation of efficiencies of transformation in different libraries Destination: B12 Item: Title: Sequencing of transposon libraries Destination: B13 Item: Title: Definition of a M. pneumoniae annotation and intergenic database covering sequence features, -omics measures Destination: B14 Item: Title: Transposon insertion sites calling and visualization Destination: B15 Item: Title: Genome-base level labeling to explore transposition selection Destination: B16 Item: Title: Identification analysis by ProTInSeq Destination: B17 Item: Title: Identification analysis by ribosome profiling Destination: B18 Item: Title: Definition of a curated set of SEPs in Mycoplasma pneumoniae and predicted functional properties Destination: B19 Item: Title: Reporting summary Destination: B20 Item: Title: Data availability Destination: M1 Item: Title: Code availability Destination: M2 Item: Title: References Destination: A4 Item: Title: Acknowledgements Destination: A5 Item: Title: Author contributions Destination: A6 Item: Title: Competing interests Destination: A7 Item: Title: Additional information Destination: M3 Info: Title: ProTInSeq: transposon insertion tracking by ultra-deep DNA sequencing to identify translated large and small ORFs Author: Samuel Miravet-Verde Subject: Nature Communications, doi:10.1038/s41467-024-46112-2 Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue Mar 26 04:07:12 CET 2024 ModDate: Thu Mar 28 07:20:47 CET 2024 ID: 0x874b1105a94ba70af88f116af1a9ae98, 0x45f461d4ba6bfa9a9f4b3c96306a6854 Filters: FilterPipeline: FlateDecode 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