5d23d8b79361393e96e2973531c07f6d pmc_10931159.pdf d0e2b3c51c71e95db9bd9e3d473db87ffad35b0b pmc_10931159.pdf c354ce38559e613342ff6f9c512aa34d261003c6ad7914b26ab3a49edeeeae8b pmc_10931159.pdf Title: Validation of a Portable Fluorescence Spectroscopy System to Monitor Heat Damage in Industrially Processed Milk Subject: Heat treatments play a critical role in ensuring the safety and preservation of milk, but it can affect its nutritional and sensory properties. The present paper proposes the use of a portable system based on fluorescence spectroscopy as an alternative method for the quantification of four thermal damage markers at once (hydroxymethylfurfural, sulfhydryl groups, ascorbic acid, and riboflavin). The obtained prediction models using autofluorescent compounds (tryptophan, dityrosine, Maillard compounds, and riboflavin), validated with skimmed milk processed under several industrial conditions, granted the development of a portable and/or online system, allowing for the real-time monitoring of thermal damage and control of the heat treatment process. The results of this study will certainly contribute to the development of new process analytical technologies for the dairy industry, enabling quality control and adjustment of the manufacturing process to ensure safe and high-quality products. Keywords: fluorescent compounds; heat damage; quantification; spectrofluorometer; milk; prediction Author: Ulises Alvarado, Anna Zamora, Oscar Arango, Jordi Saldo and Manuel Castillo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Sat Mar 2 07:59:05 2024 CET ModDate: Sat Mar 2 08:03:04 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 286049 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QEACAK+VnURWPalladioL Type 1 Custom yes yes yes 10 0 IELJHY+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 NIBWRU+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 QBSATB+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 WKRYTB+EURM10 Type 1 Builtin yes yes yes 32 0 XBNVAE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 UZKIAN+CMEX10 Type 1 Builtin yes yes yes 68 0 XFIZIB+CMSY10 Type 1 Builtin yes yes yes 73 0 URFODN+CMR10 Type 1 Builtin yes yes yes 78 0 EQJVKE+EURB10 Type 1 Builtin yes yes yes 92 0 RDUGED+VnURWPalladioL-Italic Type 1 Custom yes yes yes 109 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-03-22 02:44:57 CET RepresentationInformation: pmc_10931159.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-03-20 02:30:53 CET Size: 286049 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 259 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Reception of Milk Destination: subsection.2.1 Item: Title: Heat Treatments in Industrial Conditions Destination: subsection.2.2 Item: Title: Quantification of Thermal Damage Markers Destination: subsection.2.3 Children: Item: Title: Hydroxymethylfurfural Destination: subsubsection.2.3.1 Item: Title: Sulfhydryl Groups Destination: subsubsection.2.3.2 Item: Title: Riboflavin Destination: subsubsection.2.3.3 Item: Title: Ascorbic Acid Destination: subsubsection.2.3.4 Item: Title: Fluorescence Determination Destination: subsection.2.4 Item: Title: Obtaining Prediction Models Using Fluorescent Compounds Destination: subsection.2.5 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Analysis of Variance of Thermal Damage Markers Destination: subsection.3.1 Item: Title: Prediction Models for Indicators of Thermal Damage in Milk Destination: subsection.3.2 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.5 Info: Title: Validation of a Portable Fluorescence Spectroscopy System to Monitor Heat Damage in Industrially Processed Milk Author: Ulises Alvarado, Anna Zamora, Oscar Arango, Jordi Saldo and Manuel Castillo Subject: Heat treatments play a critical role in ensuring the safety and preservation of milk, but it can affect its nutritional and sensory properties. The present paper proposes the use of a portable system based on fluorescence spectroscopy as an alternative method for the quantification of four thermal damage markers at once (hydroxymethylfurfural, sulfhydryl groups, ascorbic acid, and riboflavin). The obtained prediction models using autofluorescent compounds (tryptophan, dityrosine, Maillard compounds, and riboflavin), validated with skimmed milk processed under several industrial conditions, granted the development of a portable and/or online system, allowing for the real-time monitoring of thermal damage and control of the heat treatment process. The results of this study will certainly contribute to the development of new process analytical technologies for the dairy industry, enabling quality control and adjustment of the manufacturing process to ensure safe and high-quality products. 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