7336babf50b2f3c2f7a81b04de5969d3 pmc_11011062.pdf 47dd421cb4a9d9b592d122e48d3abdd38bc57cd7 pmc_11011062.pdf 4f3af3abde728c535edcfa5fac7cac20ac147bc391390f18457da2beada3ba85 pmc_11011062.pdf Title: Leishmania Infection in Wild Lagomorphs and Domestic Dogs in North-East Spain Subject: Leishmania infantum is a zoonotic protozoan parasite distributed worldwide that is transmitted by phlebotomine sandflies. Dogs are the main reservoir for human infections. However, in recent years, the capacity of lagomorphs to contribute to Leishmania transmission has been confirmed. The present study aimed to assess Leishmania spp. exposure and infection in lagomorphs and sympatric domestic dogs in NE Spain. Sera from European hares, European rabbits, and rural dogs were tested for antibodies against L. infantum using an in-house indirect ELISA. PCR analysis targeting Leishmania spp. was performed in spleens from L. europaeus. Antibodies against Leishmania spp. were detected in all the species analyzed. Total sample prevalence was significantly higher in O. cuniculus (27.9%) than in L. europaeus (2.0%). Results of the PCR were all negative. The present study expands knowledge about Leishmania infections in free-ranging lagomorphs in the Iberian Peninsula, suggesting a more important role of O. cuniculus in the study area. Given the strong correlation between lagomorph densities and human leishmaniasis outbreaks in Spain, the high rabbit and human densities in NE Spain, and the high Leishmania spp. seroprevalence in rabbits, it becomes imperative to establish surveillance programs for lagomorphs in this region. Keywords: Canis familiaris; Catalonia; leishmaniosis; Leishmania infantum; Lepus europaeus; Oryctolagus cuniculus; seroprevalence; wildlife Author: Oscar Cabezón, Pamela Martínez-Orellana, Maria Puig Ribas, Catarina Jota Baptista, Diana Gassó, Roser Velarde, Xavier Fernández Aguilar and Laia Solano-Gallego Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Tue Apr 2 15:22:26 2024 CEST ModDate: Tue Apr 2 15:25:10 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 502094 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 ITOYSK+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 FQSIZK+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 VEJDPA+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 YBTHOU+URWPalladioL-BoldItal Type 1 Custom yes yes yes 32 0 IYDCBB+VnURWPalladioL Type 1 Custom yes yes yes 37 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 WVIODX+CMSY10 Type 1 Builtin yes yes yes 70 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 78 0 NJWNDJ+VnURWPalladioL-Italic Type 1 Custom yes yes yes 104 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-04-25 02:42:55 CEST RepresentationInformation: pmc_11011062.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-04-24 02:31:15 CEST Size: 502094 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 285 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals and Samples Destination: subsection.2.1 Item: Title: Serological and Molecular Analyses Destination: subsection.2.2 Item: Title: Review of Leishmania Infection in Wild Lagomorphs in Spain Destination: subsection.2.3 Item: Title: Statistical Analysis Destination: subsection.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: Serological and Molecular Analyses Destination: subsection.3.1 Item: Title: Leishmania infantum in Lagomorphs in Spain Destination: subsection.3.2 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Leishmania Infection in Wild Lagomorphs and Domestic Dogs in North-East Spain Author: Oscar Cabezón, Pamela Martínez-Orellana, Maria Puig Ribas, Catarina Jota Baptista, Diana Gassó, Roser Velarde, Xavier Fernández Aguilar and Laia Solano-Gallego Subject: Leishmania infantum is a zoonotic protozoan parasite distributed worldwide that is transmitted by phlebotomine sandflies. Dogs are the main reservoir for human infections. However, in recent years, the capacity of lagomorphs to contribute to Leishmania transmission has been confirmed. The present study aimed to assess Leishmania spp. exposure and infection in lagomorphs and sympatric domestic dogs in NE Spain. Sera from European hares, European rabbits, and rural dogs were tested for antibodies against L. infantum using an in-house indirect ELISA. PCR analysis targeting Leishmania spp. was performed in spleens from L. europaeus. Antibodies against Leishmania spp. were detected in all the species analyzed. Total sample prevalence was significantly higher in O. cuniculus (27.9%) than in L. europaeus (2.0%). Results of the PCR were all negative. The present study expands knowledge about Leishmania infections in free-ranging lagomorphs in the Iberian Peninsula, suggesting a more important role of O. cuniculus in the study area. Given the strong correlation between lagomorph densities and human leishmaniasis outbreaks in Spain, the high rabbit and human densities in NE Spain, and the high Leishmania spp. seroprevalence in rabbits, it becomes imperative to establish surveillance programs for lagomorphs in this region. 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