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Title:           PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T follicular helper cells in lupus
Subject:         Nature Communications, doi:10.1038/s41467-024-47691-w
Keywords:        
Author:          John TCHEN
Creator:         Springer
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     Item: 
      Title: PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T’follicular helper cells in’lupus
      Destination: title_link
      Children: 
       Item: 
        Title: Results
        Destination: A1
        Children: 
         Item: 
          Title: Human blood basophils from SLE patients overexpress PD-L1
          Destination: B1
         Item: 
          Title: Basophil-TFH cell axis in lupus-like mouse’models
          Destination: B2
         Item: 
          Title: Basophils control TFH cell ability to produce IL-21 and IL-4 in lupus-like’models
          Destination: B3
         Item: 
          Title: Basophils promote ex’vivo TFH cell differentiation through IL-4- and PD-L1-dependent mechanisms
          Destination: B4
         Item: 
          Title: CD4+ T’cells promote basophil ability to induce TFH cell differentiation ex’vivo
          Destination: B5
         Item: 
          Title: PD-L1 controls the basophil-TFH cell functional relationship during lupus-like disease
          Destination: B6
         Item: 
          Title: Basophil-derived IL-4 exerts a dual effect during lupus-like disease development
          Destination: B7
         Item: 
          Title: IL-4 and IL-3 control PD-L1 expression, activation and localization of basophils in the lupus-like context
          Destination: B8
         Item: 
          Title: Human basophils drive ex’vivo TFH cell and TFH2 cell differentiation through IL-4, IL-6, and PD-1-dependent mechanisms
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       Item: 
        Title: Discussion
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       Item: 
        Title: Methods
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        Children: 
         Item: 
          Title: Patient recruitment and’ethics
          Destination: B10
         Item: 
          Title: Human sample handling
          Destination: B11
         Item: 
          Title: Mice
          Destination: B12
         Item: 
          Title: Pristane-induced lupus-like mouse’model
          Destination: B13
         Item: 
          Title: DT-mediated basophil depletion in lupus-like context
          Destination: B14
         Item: 
          Title: Mouse OVA immunization experiments
          Destination: B15
         Item: 
          Title: Anti-IL-3 and anti-IL-4 antibodies treatment
          Destination: B16
         Item: 
          Title: Mouse sample processing
          Destination: B17
         Item: 
          Title: Ex vivo stimulation of splenocytes
          Destination: B18
         Item: 
          Title: Flow cytometry staining
          Destination: B19
         Item: 
          Title: Kidney Immunofluorescence’assays
          Destination: B20
         Item: 
          Title: Spleen immunofluorescence’assays
          Destination: B21
         Item: 
          Title: Anti-RNP IgG and IgM autoantibody detection
          Destination: B22
         Item: 
          Title: Anti-dsDNA IgG detection
          Destination: B23
         Item: 
          Title: Cell sorting and co-culture
          Destination: B24
         Item: 
          Title: Reverse transcription and quantitative polymerase chain reaction (RT-qPCR)
          Destination: B25
         Item: 
          Title: Statistical analysis
          Destination: B26
         Item: 
          Title: Material availability
          Destination: B27
         Item: 
          Title: Reporting summary
          Destination: B28
       Item: 
        Title: Data availability
        Destination: M1
       Item: 
        Title: References
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       Item: 
        Title: Acknowledgements
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       Item: 
        Title: Author contributions
        Destination: A6
       Item: 
        Title: Competing interests
        Destination: A7
       Item: 
        Title: Additional information
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