06e6639019d99582bc3882d6b8af247f pmc_11052431.pdf 1f43d251a0ee9b92d785cfc4f1cb47bc9927ee24 pmc_11052431.pdf 13a070167790a9a2fc5b25ddc0ad9f59968f542dbf570dc33f8d93bb8de0cb56 pmc_11052431.pdf Title: Comparison of the Effect of Two Different Handling Conditions at Slaughter in Saliva Analytes in Pigs Subject: In this report, different handling conditions at slaughterhouse were studied to assess changes in salivary biomarkers. For this purpose, finishing pigs were divided into two groups, one in which handling was improved to minimize stress (Group A, n = 24, transported and stabled at the slaughterhouse at low density without mixing with unfamiliar animals throughout the whole process) and another one in which animals had a more stressful handling process (Group B, n = 24, transported and stabled at high density with unfamiliar animals). Saliva samples were taken the day before transport to the slaughterhouse at 8:00 a.m. (B0) and 12:00 a.m. (B4), and the day of slaughter just after unloading animals at the slaughterhouse at approximately 8:00 a.m. (S0) and after 4 h of lairage at approximately 12:00 a.m. (S4). Group B showed significantly higher cortisol, total esterase activity, oxytocin, adenosine deaminase and haptoglobin levels than the Group A at both S0 and S4 sampling times, and higher levels of calprotectin and creatine kinase at S4 sampling time. This report indicates that differences in the way in which the pigs are handled at the slaughterhouse can lead to changes in salivary biomarkers and opens the possibility of the use of biomarker at slaughter to monitor handling conditions. Keywords: biomarkers; porcine; saliva; slaughterhouse; welfare Author: María Botía, Damián Escribano, Alba Ortín-Bustillo, María J. López-Martínez, Pablo Fuentes, Francisco J. Jiménez-Caparrós, Juan L. Hernández-Gómez, Antonio Avellaneda, José J. Cerón, Camila P. 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Info: Title: Comparison of the Effect of Two Different Handling Conditions at Slaughter in Saliva Analytes in Pigs Author: María Botía, Damián Escribano, Alba Ortín-Bustillo, María J. López-Martínez, Pablo Fuentes, Francisco J. Jiménez-Caparrós, Juan L. Hernández-Gómez, Antonio Avellaneda, José J. Cerón, Camila P. Rubio, Asta Tvarijonaviciute, Silvia Martínez-Subiela, Marina López-Arjona and Fernando Tecles Subject: In this report, different handling conditions at slaughterhouse were studied to assess changes in salivary biomarkers. For this purpose, finishing pigs were divided into two groups, one in which handling was improved to minimize stress (Group A, n = 24, transported and stabled at the slaughterhouse at low density without mixing with unfamiliar animals throughout the whole process) and another one in which animals had a more stressful handling process (Group B, n = 24, transported and stabled at high density with unfamiliar animals). Saliva samples were taken the day before transport to the slaughterhouse at 8:00 a.m. (B0) and 12:00 a.m. (B4), and the day of slaughter just after unloading animals at the slaughterhouse at approximately 8:00 a.m. (S0) and after 4 h of lairage at approximately 12:00 a.m. (S4). Group B showed significantly higher cortisol, total esterase activity, oxytocin, adenosine deaminase and haptoglobin levels than the Group A at both S0 and S4 sampling times, and higher levels of calprotectin and creatine kinase at S4 sampling time. This report indicates that differences in the way in which the pigs are handled at the slaughterhouse can lead to changes in salivary biomarkers and opens the possibility of the use of biomarker at slaughter to monitor handling conditions. 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