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PageMode: UseOutlines Language: en Outlines: Item: Title: Challenges and opportunities in processing NanoString nCounter data Destination: csbj2627.title.0:1 Children: Item: Title: 1 Introduction Destination: csbj2627.section.1:2 Children: Item: Title: 1.1 NanoString nCounter workflow Destination: csbj2627.subsection.1:3 Item: Title: 1.2 Detection and quantification of mRNA Destination: csbj2627.subsection.2:5 Item: Title: 1.3 Detection and quantification of miRNA Destination: csbj2627.subsection.3:6 Item: Title: 1.4 Prevalence of NanoString nCounter Destination: csbj2627.subsection.4:7 Item: Title: 1.5 Advantages and limitations Destination: csbj2627.subsection.5:9 Item: Title: 2 nCounter data processing Destination: csbj2627.section.2:10 Children: Item: Title: 2.1 Sources of bias and noise in nCounter data Destination: csbj2627.subsection.1:11 Item: Title: 2.2 NanoString nSolver software for nCounter data processing Destination: csbj2627.subsection.2:12 Item: Title: 2.3 Data pre-processing Destination: csbj2627.subsection.3:15 Item: Title: 2.4 Quality control Destination: csbj2627.subsection.4:16 Item: Title: 2.5 Background correction Destination: csbj2627.subsection.5:17 Item: Title: 2.6 Normalization Destination: csbj2627.subsection.6:18 Item: Title: 2.7 Differential expression Destination: csbj2627.subsection.7:19 Item: Title: 3 Conclusions Destination: csbj2627.section.3:20 Item: Title: CRediT authorship contribution statement Destination: csbj2627.section.3:21 Item: Title: Declaration of competing interest Destination: csbj2627.section.3:22 Item: Title: Acknowledgements Destination: csbj2627.section.3:23 Item: Title: References Destination: csbj2627.section.3:24 Info: Title: Challenges and opportunities in processing NanoString nCounter data Author: Jarosław Chilimoniuk Subject: Computational and Structural Biotechnology Journal, 23 (2024) 1951-1958. doi:10.1016/j.csbj.2024.04.061 Keywords: NanoString,nSolver,mRNA,miRNA,nCounter,Background correction,Normalization,Differential expression Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Dec 16 12:00:58 CET 2024 ModDate: Tue Dec 17 15:08:04 CET 2024 ID: 0xe5d76cfe50659e1319c3fb6c5cccebe1, 0xb4cec3c10c83f57e6afbef0510b2c31f Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 246 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 237 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 231 ImageHeight: 165 BitsPerSample: 8 BitsPerSampleUnit: integer Interpolate: true Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 239 ImageHeight: 171 BitsPerSample: 8 BitsPerSampleUnit: integer Interpolate: true Image: NisoImageMetadata: 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Computational and Structural Biotechnology Journal, 23 (2024) 1951-1958. doi:10.1016/j.csbj.2024.04.061 NanoString nSolver mRNA miRNA nCounter Background correction Normalization Differential expression Challenges and opportunities in processing NanoString nCounter data Jarosław Chilimoniuk Anna Erol Stefan Rödiger Michał Burdukiewicz journal Computational and Structural Biotechnology Journal © 2024 The Author(s). 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