421d0ac758913f7e0411633b9ed322b8 pmc_11130253.pdf bf06a52495d650fee1eb77e0b80e79a491b05233 pmc_11130253.pdf 6658f3e8b5a836bd29919f7652fd204fc8bd3b20b0c2e34b9b3414138aaee17d pmc_11130253.pdf Title: Broad-spectrum ubiquitin/ubiquitin-like deconjugation activity of the rhizobial effector NopD from Bradyrhizobium (sp. XS1150) Subject: Communications Biology, doi:10.1038/s42003-024-06344-w Keywords: Author: Ying Li Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Mon May 27 11:51:59 2024 CEST ModDate: Mon May 27 14:10:07 2024 CEST Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 790.866 pts Page rot: 0 File size: 2368332 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FOALMC+AdvOT3a037357.I Type 1C WinAnsi yes yes no 334 0 FOAKFI+AdvOT9d7a08b4.BI Type 1C WinAnsi yes yes no 622 0 FOALMD+AdvOT1ef757c0 Type 1C WinAnsi yes yes no 60 0 FOALMA+AdvOTf0129623 Type 1C WinAnsi yes yes no 62 0 FOALMB+AdvOTf0129623+fb Type 1C Custom yes yes yes 128 0 FOAKAE+AdvOT511ca30e.B Type 1C WinAnsi yes yes no 623 0 FOAKAF+AdvOT0c200bf0 Type 1C WinAnsi yes yes no 624 0 FOAKAG+AdvOT0f7080ee Type 1C WinAnsi yes yes no 625 0 FOAKAH+AdvOT34fe1490.B Type 1C WinAnsi yes yes no 73 0 FOALME+AdvOT1ef757c0+fb Type 1C Custom yes yes yes 70 0 FOAKEG+AdvOTf94803d4 Type 1C WinAnsi yes yes no 74 0 FOAKFH+AdvOTfbba3ace.B Type 1C WinAnsi yes yes no 626 0 FOAOHN+AdvOTb65e897d.B+fb Type 1C Custom yes yes yes 59 0 FOAOHO+AdvOT45bf69de.BI Type 1C WinAnsi yes yes no 61 0 FOAOGM+AdvOTb65e897d.B Type 1C WinAnsi yes yes no 63 0 FOAOEL+CourierNewPSMT Type 1C WinAnsi yes yes no 64 0 FOAOEM+CourierNewPS-BoldMT Type 1C WinAnsi yes yes no 65 0 FOAOEJ+Arial-BoldMT Type 1C WinAnsi yes yes no 66 0 FOAOEK+Arial-ItalicMT Type 1C WinAnsi yes yes no 67 0 FOAMED+AdvOT1ef757c0+20 Type 1C WinAnsi yes yes no 68 0 FOANKM+ArialMT Type 1C Custom yes yes yes 69 0 FOAOHP+AdvOT1ef757c0+03 Type 1C Custom yes yes yes 71 0 FOAMDD+AdvOT7d6df7ab.I Type 1C WinAnsi yes yes no 72 0 FOAOLA+AdvOTf0129623+20 Type 1C WinAnsi yes yes no 129 0 FOAOLB+AdvTT153188ed+03 Type 1C Custom yes yes yes 130 0 FOAOJP+AdvOT6601a1b9.BI Type 1C WinAnsi yes yes no 131 0 FOBFAL+Arial-BoldItalicMT Type 1C WinAnsi yes yes no 149 0 FOBHGN+AdvOT34fe1490.B+fb Type 1C Custom yes yes yes 228 0 FOCFHJ+AdvOT1ef757c0+22 Type 1C Custom yes yes yes 261 0 FOCFHI+AdvTT691e30a0 Type 1C WinAnsi yes yes no 262 0 FOCFIJ+AdvTTab7e17fd+22 Type 1C Custom yes yes no 314 0 FOCFKK+AdvOTa20b42a7 Type 1C WinAnsi yes yes no 335 0 FOCFNJ+AdvOT34fe1490.B+20 Type 1C WinAnsi yes yes no 352 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-05 02:51:51 CET RepresentationInformation: pmc_11130253.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-11-15 02:25:09 CET Size: 2368332 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 730 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Broad-spectrum ubiquitin/ubiquitin-like deconjugation activity of the rhizobial effector NopD from Bradyrhizobium (sp.’XS1150) Destination: title_link Children: Item: Title: Results Destination: A1 Children: Item: Title: NopD has an unusual multiple activity for SUMO, ubiquitin and’Nedd8 Destination: B1 Item: Title: Overall complex structures of NopD with Arabidopsis SUMO2 and ubiquitin Destination: B2 Item: Title: C-terminal tail comparison of SUMO2 and ubiquitin in’NopD Destination: B3 Item: Title: Unraveling the SUMO2 and ubiquitin interface with’NopD Destination: B4 Item: Title: Dissecting the deconjugation activities in tobacco leaves infiltration’assays Destination: B5 Item: Title: Discussion Destination: A2 Item: Title: Materials and methods Destination: A3 Children: Item: Title: Plasmids, Cloning and Point Mutation Destination: B6 Item: Title: Protein expression and purification Destination: B7 Item: Title: Preparation of the NopD complexes with AtSUMO2 and ubiquitin Destination: B8 Item: Title: Crystallization and data collection Destination: B9 Item: Title: Activity-based binding probe’assays Destination: B10 Item: Title: AMC-substrate hydrolysis’assays Destination: B11 Item: Title: In vitro de-ubiquitination’assays Destination: B12 Item: Title: In vitro de-SUMOyation’assays Destination: B13 Item: Title: Cell-Death Tobacco Leaves’assays Destination: B14 Item: Title: Statistics and reproducibility Destination: B15 Item: Title: Data availability Destination: A4 Item: Title: References Destination: A5 Item: Title: Acknowledgements Destination: A6 Item: Title: Author contributions Destination: A7 Item: Title: Competing interests Destination: A8 Item: Title: Additional information Destination: M3 Info: Title: Broad-spectrum ubiquitin/ubiquitin-like deconjugation activity of the rhizobial effector NopD from Bradyrhizobium (sp. 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XS1150) Ying Li Jordi Perez-Gil L. Maria Lois Nathalia Varejão David Reverter Springer US Communications Biology, doi:10.1038/s42003-024-06344-w 10.1038/s42003-024-06344-w http://dx.doi.org/10.1038/s42003-024-06344-w journal Communications Biology © 2024, The Author(s) 2399-3642 10.1038/s42003-024-06344-w springer.com springerlink.com 2010-04-23 True 10.1038/s42003-024-06344-w springer.com springerlink.com Springer 2024-05-27T14:10:07+02:00 2024-05-27T15:21:59+05:30 2024-05-27T14:10:07+02:00 True VoR uuid:6c71f420-0bf0-4ede-8192-6fc76ab372b1 uuid:902c9e6c-4937-49f2-8ccb-d8668fa4328e default 1 converted uuid:bb5249ce-f679-43a9-ae99-e09b927dd017 converted to PDF/A-2b pdfToolbox 2024-05-27T15:23:18+05:30 2 B Ying Li http://orcid.org/0000-0002-5377-6262 Jordi Perez-Gil http://orcid.org/0000-0002-5632-9556 L. 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