6227c8acbbe556831d590fb22c81c9fb pmc_11200434.pdf 200ccd6c41a7d99792c4a1bbe9b2469656383a5c pmc_11200434.pdf 37651cf8d0ec43a625b23b4727aba3fc0f7ba1dfba8ff97c8ef25c52e93164a7 pmc_11200434.pdf Title: Transcriptional Evaluation of Neuropeptides, Hormones, and Tissue Repair Modulators in the Skin of Gilthead Sea Bream (Sparus aurata L.) Subjected to Mechanical Damage Subject: The skin of bony fish is the first physical barrier and is responsible for maintaining the integrity of the fish. Lesions make the skin vulnerable to potential infection by pathogens present in the aquatic environment. In this way, wound repair has barely been studied in gilthead sea bream. Thus, this study investigated the modulation of peripheral neuro-endocrine and tissue repair markers at the transcriptional level in the skin of teleost fish subjected to mechanical damage above or below the lateral line (dorsal and ventral lesions, respectively). Samples were evaluated using RT-qPCR at 2-, 4-, and 20-days post-injury. Fish with a ventral lesion presented a trend of progressive increase in the expressions of corticotropin-releasing hormone (crh), pro-opiomelanocortin-A (pomca), proenkephalin-B (penkb), cholecystokinin (cck), oxytocin (oxt), angiotensinogen (agt), and (less pronounced) somatostatin-1B (sst1b). By contrast, fish with a dorsal lesion registered no significant increase or biological trend for the genes evaluated at the different sampling times. Collectively, the results show a rapid and more robust response of neuro-endocrine and tissue repair markers in the injuries below than above the lateral line, which could be attributable to their proximity to vital organs. 20pt Keywords: Sparus aurata; skin wound; tissue repair; stress; neuro-immuno-endocrine axis; immune system; aquaculture Author: Rocío Piñera-Moreno, Felipe E. Reyes-López, Merari Goldstein, María Jesús Santillán-Araneda, Bárbara Robles-Planells, Camila Arancibia-Carvallo, Eva Vallejos-Vidal, Alberto Cuesta, María Ángeles Esteban and Lluis Tort Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Jun 24 07:18:16 2024 CEST ModDate: Mon Jun 24 07:19:05 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2844520 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TAGTVB+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 KQTRYY+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 ZIAKDN+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 AXIINI+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 SEZNRU+URWPalladioL-BoldItal Type 1 Custom yes yes yes 32 0 QOONAA+VnURWPalladioL Type 1 Custom yes yes yes 37 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 LEVXPH+EURM10 Type 1 Builtin yes yes yes 70 0 RMKEMV+CMSY10 Type 1 Builtin yes yes yes 78 0 DUJUUF+PazoMath-Italic Type 1 Builtin yes yes yes 86 0 LGANOC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 115 0 LGANOD+PalatinoLinotype CID TrueType Identity-H yes yes yes 118 0 LGANOE+PalatinoLinotype TrueType WinAnsi yes yes no 124 0 LGANOG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 127 0 LGAOFG+PalatinoLinotype TrueType MacRoman yes yes no 130 0 LGANOC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 202 0 LGANOE+PalatinoLinotype TrueType WinAnsi yes yes no 205 0 LGANOG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 208 0 LGAOFG+PalatinoLinotype TrueType MacRoman yes yes no 211 0 LGANOC+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 253 0 LGANOD+PalatinoLinotype CID TrueType Identity-H yes yes yes 256 0 LGANOE+PalatinoLinotype TrueType WinAnsi yes yes no 262 0 LGANOG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 265 0 LGAOFG+PalatinoLinotype TrueType MacRoman yes yes no 268 0 MPUDFL+PazoMath Type 1 Builtin yes yes yes 317 0 GEJNTL+VnURWPalladioL-Italic Type 1 Custom yes yes yes 322 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-09-01 08:12:46 CEST RepresentationInformation: pmc_11200434.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-07-18 02:30:15 CEST Size: 2844520 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 560 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Fish and Rearing Condition Destination: subsection.2.1 Item: Title: Experimental Design Destination: subsection.2.2 Item: Title: Sampling Destination: subsection.2.3 Item: Title: Isolation of RNA and cDNA Synthesis Destination: subsection.2.4 Item: Title: Quantitative Real-Time PCR (qPCR) Destination: subsection.2.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Transcriptional Evaluation of Neuropeptides, Hormones, and Tissue Repair Modulators in the Skin of Gilthead Sea Bream (Sparus aurata L.) Subjected to Mechanical Damage Author: Rocío Piñera-Moreno, Felipe E. Reyes-López, Merari Goldstein, María Jesús Santillán-Araneda, Bárbara Robles-Planells, Camila Arancibia-Carvallo, Eva Vallejos-Vidal, Alberto Cuesta, María Ángeles Esteban and Lluis Tort Subject: The skin of bony fish is the first physical barrier and is responsible for maintaining the integrity of the fish. Lesions make the skin vulnerable to potential infection by pathogens present in the aquatic environment. In this way, wound repair has barely been studied in gilthead sea bream. Thus, this study investigated the modulation of peripheral neuro-endocrine and tissue repair markers at the transcriptional level in the skin of teleost fish subjected to mechanical damage above or below the lateral line (dorsal and ventral lesions, respectively). Samples were evaluated using RT-qPCR at 2-, 4-, and 20-days post-injury. Fish with a ventral lesion presented a trend of progressive increase in the expressions of corticotropin-releasing hormone (crh), pro-opiomelanocortin-A (pomca), proenkephalin-B (penkb), cholecystokinin (cck), oxytocin (oxt), angiotensinogen (agt), and (less pronounced) somatostatin-1B (sst1b). By contrast, fish with a dorsal lesion registered no significant increase or biological trend for the genes evaluated at the different sampling times. 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