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Introduction Destination: flink1 Item: Title: 2. Materials and methods Destination: flink2 Children: Item: Title: 2.1. Bacterial strains, plasmids and oligonucleotides Destination: slink1 Item: Title: 2.2. Mycoplasma sample preparation and total protein quantification Destination: slink2 Item: Title: 2.3. Time-course experiments Destination: slink3 Item: Title: 2.4. Proteome analysis by protein mass spectroscopy (MS) Destination: slink4 Item: Title: 2.5. Dot blot Destination: slink5 Item: Title: 2.6. Analysis of lipid and glycolipid content in M. pneumoniae cell extracts by liquid chromatography-high resolution mass spect ... Destination: slink6 Item: Title: 2.7. Serum reactivity with M. pneumoniae glycolipids Destination: slink7 Item: Title: 2.8. Inibition ELISA assay Destination: slink8 Item: Title: 3. Results Destination: flink3 Children: Item: Title: 3.1. Glycolipid metabolic map of M. pneumoniae and its essentiality Destination: slink9 Item: Title: 3.2. Engineering M. pneumoniae glycolipid biosynthetic pathway to avoid the production of galactolipids Destination: slink10 Item: Title: 3.3. Growth analysis and proteomic characterisation of strains engineered to impair galactolipid production Destination: slink11 Item: Title: 3.4. Glycolipid profiling of the different strains Destination: slink12 Item: Title: 3.5. Analysis of the cross-reactivity of the different strains with GalCer using acute phase serum from patients with GBS Destination: slink13 Item: Title: 4. Discussion Destination: flink4 Item: Title: Declaration of competing interest Destination: aclink4 Item: Title: Acknowledgments Destination: aclink5 Item: Title: Appendix A. Supplementary data Destination: flink5 Item: Title: References Destination: blink1 Info: Title: Engineering Mycoplasma pneumoniae to bypass the association with Guillain-BarrE syndrome Author: Alicia Broto Subject: Microbes and Infection, 26 (2024) 105342. doi:10.1016/j.micinf.2024.105342 Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Wed Jul 03 00:20:18 CEST 2024 ModDate: Wed Jul 03 00:23:25 CEST 2024 ID: 0xc7ed95d19a2ac0aecac82706f2fb7195, 0x696f80511280814492c75e93e722f1f4 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 120 ImageHeight: 119 ColorSpace: palette color BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 237 ImageHeight: 299 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 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FirstChar: 40 LastChar: 121 FontDescriptor: FontName: EEGCMD+AdvOTb83ee1dd.B Flags: Nonsymbolic FontBBox: -93, -250, 1041, 989 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: EEGCLO+AdvTT5843c571 FontSubset: true FirstChar: 42 LastChar: 42 FontDescriptor: FontName: EEGCLO+AdvTT5843c571 Flags: Nonsymbolic FontBBox: -72, -208, 927, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: EEGDCH+AdvOTb0c9bf5d+21 FontSubset: true FirstChar: 153 LastChar: 153 FontDescriptor: FontName: EEGDCH+AdvOTb0c9bf5d+21 Flags: Nonsymbolic FontBBox: -93, -250, 1145, 864 FontFile3: true Encoding: WinAnsiEncoding XMP: application/pdf doi:10.1016/j.micinf.2024.105342 Engineering Mycoplasma pneumoniae to bypass the association with Guillain-BarrE syndrome Alicia Broto Carlos Piñero-Lambea Carolina Segura-Morales Anne P. Tio-Gillen Wendy W.J. Unger Raul Burgos Rocco Mazzolini Samuel Miravet-Verde Bart C. Jacobs Josefina Casas Ruth Huizinga Maria Lluch-Senar Luis Serrano Mycoplasma pneumoniae Glycolipids Galactocerebrosides Glycosyltransferases Immune response Molecular mimicry Microbes and Infection, 26 (2024) 105342. doi:10.1016/j.micinf.2024.105342 The Authors journal Microbes and Infection © 2024 The Authors. 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SHA-1 Checksum: 65cd76bd51eec03550c5eca65e4baae7cedd899cff38d68f2518c5528cd9828a Type: SHA-256