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Author: Clara Marin; Lourdes Migura-García; Juan Carlos Rodríguez; María-Paz Ventero; Maria Teresa Pérez-Gracia; Santiago Vega; Carla Tort-Miró; Ana Marco-Fuertes; Laura Lorenzo-Rebenaque; Laura Montoro-Dasi Creator: Adobe InDesign 14.0 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Fri Jun 28 16:58:47 2024 CEST ModDate: Fri Jun 28 16:59:00 2024 CEST Custom Metadata: no Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1281156 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KUSZME+MuseoSans-500 Type 1C Custom yes yes yes 8 0 HNEHWK+MuseoSans-300 Type 1C Custom yes yes yes 47 0 FKFVUO+MuseoSans-700 Type 1C Custom yes yes yes 9 0 ELQPGQ+MuseoSans-300Italic Type 1C WinAnsi yes yes yes 129 0 DHBJSS+MinionPro-Regular Type 1C Custom yes yes yes 10 0 ZBDLOA+MinionPro-It Type 1C Custom yes yes yes 11 0 WUPTYG+STIX-Regular Type 1C Custom yes yes yes 45 0 URQHWK+STIXGeneral-Regular Type 1C Custom yes yes yes 46 0 TSBBIM+GentiumPlus CID TrueType Identity-H yes yes yes 61 0 QLNJSS+TimesNewRomanPS-BoldItalicMT Type 1C Custom yes yes no 62 0 QLNJSS+TimesNewRomanPS-BoldMT Type 1C Custom yes yes no 63 0 PMDDEU+SymbolMT Type 1C Custom yes yes no 64 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-23 02:08:19 CET RepresentationInformation: pmc_11252001.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-22 02:06:42 CET Size: 1281156 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Tagged PDF PDFMetadata: Objects: 2116 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-GB Outlines: Item: Title: Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status Children: Item: Title: 1 Introduction Item: Title: 2 Materials and methods Children: Item: Title: 2.1 Study sample Item: Title: 2.2 Selection of high and low sanitary status farms to define “healthy” and “unhealthy” microbiomes Item: Title: 2.3 Sampling Item: Title: 2.4 DNA extraction, 16S rRNA gene amplification, and MiSeq sequencing Item: Title: 2.5 Bioinformatic analysis Item: Title: 2.6 Statistical analysis Item: Title: 2.7 Bacterial functional annotation and distribution Item: Title: 3 Results Children: Item: Title: 3.1 16S rRNA sequencing Item: Title: 3.2 Taxonomic characterisation of environmental microbial communities Item: Title: 3.3 Sanitary status environmental microbiota modulation Item: Title: 3.4 Bacterial functional annotation and distribution Item: Title: 4 Discussion Item: Title: Data availability statement Item: Title: Author contributions Item: Title: References Info: Title: Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status Author: Clara Marin; Lourdes Migura-García; Juan Carlos Rodríguez; María-Paz Ventero; Maria Teresa Pérez-Gracia; Santiago Vega; Carla Tort-Miró; Ana Marco-Fuertes; Laura Lorenzo-Rebenaque; Laura Montoro-Dasi Subject: Introduction: Stringent regulations in pig farming, such as antibiotic control and the ban on certain additives and disinfectants, complicate disease control efforts. 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