96a78f0935ad824666c8afa054143f27 pmc_11334063.pdf 598bb968451e50ec584b7bc277b8207a0d56a696 pmc_11334063.pdf c992a767b2040c08a55c9aae0a932507e7b900348cc98304df1fd11903852fd4 pmc_11334063.pdf Title: Usefulness of Xpert MTB/RIF and Xpert Ultra to Categorize Risk of Tuberculosis Transmission to Household Contacts Subject: DOI: 10.1093/ofid/ofae450; Open Forum Infectious Diseases, 11, 8, 2024-08-06.; Abstract: Background: People with pulmonary tuberculosis (PTB) are contagious, particularly to their household contacts. Their infectivity has been associated with the bacterial load in sputum samples. This study investigated if the bacterial load in sputum samples as quantified by Xpert MTB/RIF and Xpert Ultra is correlated with the extent that latent tuberculosis infection (LTBI) occurred in household contacts of people with PTB. Methods: A retrospective study was performed including people with PTB presenting at Vall d’Hebron University Hospital, Barcelona, between 2011 and 2021. Their infection ratio, representing the proportion of household members found with LTBI in contact tracing investigation, was compared with the quantitative results of Xpert MTB/RIF and Xpert Ultra using ordinal regression analysis. Results: A total of 107 people with PTB were included. Among their 398 household contacts, 126 (31.7%) cases of LTBI and 14 cases with active TB disease (3.5%) were reported. Higher bacterial load in Xpert MTB/RIF and Xpert Ultra baseline sputum was significantly associated with increased infection ratios, providing better estimates than conventional acid-fast bacilli (AFB) smear grading. Conclusions: Xpert MTB/RIF and Xpert Ultra could serve as an alternative to AFB sputum-smear grading in determining contact tracing priorities. Keywords: contact investigation; PCR; prevention; risk of transmission; tuberculosis Author: Alexander Mol Creator: Servigistics Arbortext Advanced Print Publisher 11.1.4546/W-x64 Producer: PDFlib+PDI 9.0.7p3 (C++/Win64) CreationDate: Tue Aug 20 04:23:07 2024 CEST ModDate: Tue Aug 20 11:29:24 2024 CEST Custom Metadata: yes Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 593.972 x 782.986 pts Page rot: 0 File size: 557669 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FPGBKC+MinionPro-Regular Type 1C Custom yes yes yes 815 0 PMOJAW+UniversLTStd-BoldCn Type 1C Custom yes yes yes 818 0 PTLCXM+UniversLTStd-LightCn Type 1C Custom yes yes yes 821 0 MYUUKF+MinionPro-BoldIt Type 1C Custom yes yes yes 824 0 YMKWTO+MinionPro-Bold Type 1C Custom yes yes yes 827 0 CVJOLN+MinionPro-It Type 1C Custom 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secofae450-s1.5 Item: Title: RESULTS Destination: secofae450-s2 Children: Item: Title: Characteristics of Included People Destination: secofae450-s2.1 Item: Title: Contact Characteristics Destination: secofae450-s2.2 Item: Title: Infection Ratios Destination: secofae450-s2.3 Item: Title: Ordinal Regression Destination: secofae450-s2.4 Item: Title: Mean Ctmin Value and AFB Smear Grading Destination: secofae450-s2.5 Item: Title: Pulmonary Cavities Destination: secofae450-s2.6 Item: Title: DISCUSSION Destination: secofae450-s3 Item: Title: CONCLUSIONS Destination: secofae450-s4 Item: Title: Supplementary Data Destination: secofae450-s5 Item: Title: Note Destination: secofae450-s6 Item: Title: References Destination: reflist Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1000 ImageHeight: 1421 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: 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Flags: Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: VILZKM+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: VILZKM+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2046, 1040 FontFile2: true XMP: uuid:8e8d1cf4-358f-4e8f-a132-0d045227cd90 uuid:E526464F-81C3-F786-BE34-CEF86E042221 True contact investigation; PCR; prevention; risk of transmission; tuberculosis PDFlib+PDI 9.0.7p3 (C++/Win64) application/pdf 10.1093/ofid/ofae450 en Oxford University Press Usefulness of Xpert MTB/RIF and Xpert Ultra to Categorize Risk of Tuberculosis Transmission to Household Contacts Alexander Mol Adrián Sánchez-Montalvá Juan Espinosa-Pereiro Maria Luisa Aznar Fernando Salvador Pau Bosch-Nicolau Maria-Luiza de Souza-Galvão María Ángeles Jiménez José Ángel Rodrigo-Pendás Joan-Pau Millet Nuria Saborit Claudia Broto Israel Molina Teresa Tórtola DOI: 10.1093/ofid/ofae450; Open Forum Infectious Diseases, 11, 8, 2024-08-06.; Abstract: Background: People with pulmonary tuberculosis (PTB) are contagious, particularly to their household contacts. Their infectivity has been associated with the bacterial load in sputum samples. This study investigated if the bacterial load in sputum samples as quantified by Xpert MTB/RIF and Xpert Ultra is correlated with the extent that latent tuberculosis infection (LTBI) occurred in household contacts of people with PTB. Methods: A retrospective study was performed including people with PTB presenting at Vall d’Hebron University Hospital, Barcelona, between 2011 and 2021. Their infection ratio, representing the proportion of household members found with LTBI in contact tracing investigation, was compared with the quantitative results of Xpert MTB/RIF and Xpert Ultra using ordinal regression analysis. Results: A total of 107 people with PTB were included. Among their 398 household contacts, 126 (31.7%) cases of LTBI and 14 cases with active TB disease (3.5%) were reported. Higher bacterial load in Xpert MTB/RIF and Xpert Ultra baseline sputum was significantly associated with increased infection ratios, providing better estimates than conventional acid-fast bacilli (AFB) smear grading. Conclusions: Xpert MTB/RIF and Xpert Ultra could serve as an alternative to AFB sputum-smear grading in determining contact tracing priorities. © The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. contact investigation PCR prevention risk of transmission tuberculosis Journal Open Forum Infectious Diseases © The Author(s) 2024. 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