ee8fce6c18096b8c4638b35f1aa30f4f pmc_11356908.pdf 906572d2dcea59829917d941c3b22b21234a083f pmc_11356908.pdf b3cb36c502a589a149d0010cea78c616e420942b18503b3b994e284c8de8da4d pmc_11356908.pdf Title: Isolation of Staphylococcus pseudintermedius in Immunocompromised Patients from a Single Center in Spain: A Zoonotic Pathogen from Companion Animals Subject: Staphylococcus pseudintermedius, a commensal opportunistic bacterium predominantly residing in the skin of companion animals, particularly dogs, has the potential to induce skin and soft tissue infections in pets, and zoonotic infections, including catheter-related complications. This study documents four cases of S. pseudintermedius infection or colonization in patients who had close contact with dogs or cats. Identification of the bacterial species was performed using MALDI-TOF mass spectrometry, and antibiotic susceptibility was determined using microdilution assay. DNA was sequenced using Nanopore technology followed by in silico analysis. Three isolates were multidrug resistant, including resistance to methicillin, with one belonging to the prevalent European lineage ST551, and the other two were attributed to a novel multilocus sequence type, ST2672. The remaining isolate was attributed to the novel multilocus sequence type ST2673 and was methicillin susceptible. All four isolates exhibited an array of virulence factors that contributed to colonization, damage to host immune cells, and biofilm formation. All the ST551 isolates included in the comparative analysis displayed clonality within the European continent. The importance of describing zoonotic infections associated with S. pseudintermedius resides in the scarcity of available scientific literature, further accentuated by its heightened resistance profile and potential complications, particularly in the context of catheter-related infections. Keywords: Staphylococcus pseudintermedius; ST551; MRSP; MDR; zoonosis; SSTI; whole-genome sequencing; catheter-related infection; nanopore; one health Author: Joaquim Viñes, Miguel Ángel Verdejo, Laura Horvath, Andrea Vergara, Jordi Vila, Olga Francino, Laura Morata, Mateu Espasa, Climent Casals-Pascual, Àlex Soriano and Cristina Pitart Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Aug 16 15:36:43 2024 CEST ModDate: Fri Aug 16 15:41:42 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2055423 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WVKMMG+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 UBEZET+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 NCEDFP+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 WONVOG+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 WETDCW+URWPalladioL-BoldItal Type 1 Custom yes yes yes 32 0 WJKERO+VnURWPalladioL Type 1 Custom yes yes yes 37 0 SIXRFS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 NBDXYZ+CMSY10 Type 1 Builtin yes yes yes 73 0 IYAOHD+PazoMath Type 1 Builtin yes yes yes 81 0 CAMXTK+EURM10 Type 1 Builtin yes yes yes 86 0 GGHNAA+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 100 0 GGHNBB+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 106 0 GGHNFB+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 112 0 GGHNGB+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 118 0 GGHNGC+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 121 0 GGHNGD+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 124 0 GGHNIF+PalatinoLinotype-Roman TrueType MacRoman yes yes no 127 0 GGHNAA+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 138 0 GGHNBB+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 144 0 GGHNFB+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 150 0 GGHNGB+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 156 0 GGHNGC+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 159 0 GGHNGD+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 162 0 GGHNAA+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 179 0 GGHNBB+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 185 0 GGHNFB+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 191 0 GGHNGB+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 197 0 GGHNGC+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 200 0 GGHNGD+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 203 0 CCQZSN+PazoMath-Italic Type 1 Builtin yes yes yes 215 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-04-02 02:46:06 CEST RepresentationInformation: pmc_11356908.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-04-01 02:19:00 CEST Size: 2055423 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 489 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Patients and Isolates Destination: subsection.2.1 Item: Title: Strain Identification and Antimicrobial Susceptibility Destination: subsection.2.2 Item: Title: DNA Extraction and Sequencing Destination: subsection.2.3 Item: Title: Genome Assembly and Analysis Destination: subsection.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: Medical History and Isolates Destination: subsection.3.1 Item: Title: Antibiotic Susceptibility Profile Destination: subsection.3.2 Item: Title: Antibiotic Resistance Genotype Destination: subsection.3.3 Item: Title: Antibiotic Resistance Genes’ Genomic Context Destination: subsection.3.4 Item: Title: Virulence Factors Destination: subsection.3.5 Item: Title: Phylogenetic and ANI Analysis Destination: subsection.3.6 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Isolation of Staphylococcus pseudintermedius in Immunocompromised Patients from a Single Center in Spain: A Zoonotic Pathogen from Companion Animals Author: Joaquim Viñes, Miguel Ángel Verdejo, Laura Horvath, Andrea Vergara, Jordi Vila, Olga Francino, Laura Morata, Mateu Espasa, Climent Casals-Pascual, Àlex Soriano and Cristina Pitart Subject: Staphylococcus pseudintermedius, a commensal opportunistic bacterium predominantly residing in the skin of companion animals, particularly dogs, has the potential to induce skin and soft tissue infections in pets, and zoonotic infections, including catheter-related complications. This study documents four cases of S. pseudintermedius infection or colonization in patients who had close contact with dogs or cats. Identification of the bacterial species was performed using MALDI-TOF mass spectrometry, and antibiotic susceptibility was determined using microdilution assay. DNA was sequenced using Nanopore technology followed by in silico analysis. Three isolates were multidrug resistant, including resistance to methicillin, with one belonging to the prevalent European lineage ST551, and the other two were attributed to a novel multilocus sequence type, ST2672. The remaining isolate was attributed to the novel multilocus sequence type ST2673 and was methicillin susceptible. All four isolates exhibited an array of virulence factors that contributed to colonization, damage to host immune cells, and biofilm formation. All the ST551 isolates included in the comparative analysis displayed clonality within the European continent. The importance of describing zoonotic infections associated with S. pseudintermedius resides in the scarcity of available scientific literature, further accentuated by its heightened resistance profile and potential complications, particularly in the context of catheter-related infections. 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