e3a9dcd71e0b480ffd3d40d699eefd31 pmc_11504221.pdf d3f5d53ff518c04fabf0fb1bfc0e5c4a6630f93d pmc_11504221.pdf 3045590907b5354f8a9843b648c678d41487f74f11effbac0e003205a9e31b74 pmc_11504221.pdf Title: Evaluation of Glutathione (GSH) System in Porcine Saliva: Validation and Application of Colorimetric Method Subject: (1) Reduced glutathione (GSH) is considered the first line of antioxidant defense. During oxidative stress, it is oxidized to glutathione disulphide (GSSG). (2) A simple and quick spectrophotometric method based on sodium borohydride (NaBH4) as a reductant to measure the total and reduced GSH in porcine saliva was analytically validated and evaluated in two situations in this species: (a) in a physiological situation, involving sows during the late lactation and post-weaning periods, and (b) in a situation of sepsis in pigs experimentally induced by LPS administration. (3) The results of the analytical validation showed that the assay was precise and accurate in the porcine saliva samples. Higher total GSH and GSSG and lower reduced GSH were observed in the saliva of sows during the post-weaning period, as well as in pigs with experimentally induced sepsis. (4) In conclusion, the validated assay showed adequate analytical results and could be used to evaluate the GSH system of porcine saliva, as demonstrated during the clinical performance. Keywords: antioxidants; biomarkers; glutathione system; saliva; validation Author: Gamze Gok, Salim Neselioglu, Heng-Lun Ko, Ozcan Erel, María José López-Martínez, Xavier Manteca and Camila Peres Rubio Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Oct 14 04:43:59 2024 CEST ModDate: Mon Oct 14 05:23:34 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 887054 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 UPBHOJ+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 ORBVBG+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 CCUKLX+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 OPZDHV+VnURWPalladioL Type 1 Custom yes yes yes 32 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 IFEDSM+CMSY10 Type 1 Builtin yes yes yes 65 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 73 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 84 0 OEKGCC+Arial,Bold CID TrueType Identity-H yes yes yes 94 0 OEKGCD+Arial CID TrueType Identity-H yes yes yes 100 0 OEKDNI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 106 0 OEKDNK+PalatinoLinotype TrueType WinAnsi yes yes no 109 0 OEKDNM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 112 0 OEKFLE+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 115 0 OEKDNI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 128 0 OEKDNJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 131 0 OEKDNK+PalatinoLinotype TrueType WinAnsi yes yes no 137 0 OEKDNM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 140 0 OEKDNI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 150 0 OEKDNJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 153 0 OEKDNK+PalatinoLinotype TrueType WinAnsi yes yes no 159 0 OEKDNM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 162 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-17 02:57:42 CET RepresentationInformation: pmc_11504221.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-12-04 02:21:35 CET Size: 887054 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 314 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Chemicals Destination: subsection.2.1 Item: Title: GSH Measurement: Total, Reduced, and Oxidized Destination: subsection.2.2 Item: Title: Glutathione Reductase (GSH Red) Measurement Destination: subsection.2.3 Item: Title: Saliva Collection Destination: subsection.2.4 Item: Title: Analytical Performance Destination: subsection.2.5 Item: Title: Applicative Studies Destination: subsection.2.6 Children: Item: Title: Sows during the Late Lactation and Post-Weaning Periods Destination: subsubsection.2.6.1 Item: Title: Experimental Sepsis Destination: subsubsection.2.6.2 Item: Title: Statistical Analysis Destination: subsection.2.7 Item: Title: Results Destination: section.3 Children: Item: Title: Analytical Performance Destination: subsection.3.1 Item: Title: Applicative Studies Destination: subsection.3.2 Children: Item: Title: Sows during the Late Lactation and Post-Weaning Periods Destination: subsubsection.3.2.1 Item: Title: Experimental Sepsis by LPS Administration Destination: subsubsection.3.2.2 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Evaluation of Glutathione (GSH) System in Porcine Saliva: Validation and Application of Colorimetric Method Author: Gamze Gok, Salim Neselioglu, Heng-Lun Ko, Ozcan Erel, María José López-Martínez, Xavier Manteca and Camila Peres Rubio Subject: (1) Reduced glutathione (GSH) is considered the first line of antioxidant defense. During oxidative stress, it is oxidized to glutathione disulphide (GSSG). (2) A simple and quick spectrophotometric method based on sodium borohydride (NaBH4) as a reductant to measure the total and reduced GSH in porcine saliva was analytically validated and evaluated in two situations in this species: (a) in a physiological situation, involving sows during the late lactation and post-weaning periods, and (b) in a situation of sepsis in pigs experimentally induced by LPS administration. (3) The results of the analytical validation showed that the assay was precise and accurate in the porcine saliva samples. Higher total GSH and GSSG and lower reduced GSH were observed in the saliva of sows during the post-weaning period, as well as in pigs with experimentally induced sepsis. (4) In conclusion, the validated assay showed adequate analytical results and could be used to evaluate the GSH system of porcine saliva, as demonstrated during the clinical performance. 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