85ddea45e55de1d79ede4823bfd7bb72 pmc_11505092.pdf e37d9573dd88ae53e5f6d25078be8f8d6fa595e6 pmc_11505092.pdf c6404d4a0fa1722a7a017d1e94ff707abbb6208f5f9a089aa4ba7e7a84724150 pmc_11505092.pdf Title: Beta-Glucans Improve the Mammary Innate Immune Response to Endotoxin Challenge in Dairy Ewes Subject: This study evaluated short-term immune responses of dairy ewes supplemented with barley -glucan (BG) following an intramammary Escherichia coli lipopolysaccharide (LPS) challenge. In the adaptation period, 36 ewes were fed an alfalfa hay diet ad libitum and barley grain cv. Hispanic (3.8% BG). Then, ewes were assigned into three experimental groups: (1) Control (CON), the same previous diet (13.3 g BG/d); (2) high -glucans barley (HBG), new barley (cv. Annapurna) containing 10% BG (35 g BG/d); (3) intraperitoneally injected (INP) with a 1.4% BG solution dose (2 g BG/ewe). At d 9, all ewes were infused with an E. coli LPS or saline solution in each udder half. After the challenge, rectal temperature (RT), milk yield and composition, somatic cell count (SCC), and plasma interleukins (IL-1 and IL-1) were monitored daily. The INP treatment revealed a transitory increase in RT and decreased milk yield by 38%. Milk fat, protein, and SCC increased in LPS-treated udders but not by BG treatment. The IL-1 plasma concentration was similar among groups but INP ewes showed a lower IL-1 concentration suggesting a lower inflammatory response. The BG administration appears more effective intraperitoneally than orally, which needs additional study. Keywords: -glucans; immunity; LPS challenge; barley; dairy sheep Author: Santiago A. Guamán, Abdelaali Elhadi, Ahmed A. K. Salama, Carmen L. Manuelian, Gerardo Caja and Elena Albanell Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Oct 21 04:36:30 2024 CEST ModDate: Mon Oct 21 04:40:16 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1243733 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 VVSYYB+EURM10 Type 1 Builtin yes yes yes 16 0 NTSDSH+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 UZEMJB+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 SQRLMQ+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 GFKJOY+VnURWPalladioL Type 1 Custom yes yes yes 37 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 YZSVFV+CMSY10 Type 1 Builtin yes yes yes 70 0 QKYDFU+MSAM10 Type 1 Builtin yes yes yes 84 0 RAMSXI+wasy9 Type 1 Builtin yes yes yes 89 0 CGNNJK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 97 0 CGNNJL+PalatinoLinotype CID TrueType Identity-H yes yes yes 100 0 CGNNJM+PalatinoLinotype TrueType WinAnsi yes yes no 106 0 CGNNJO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 109 0 CGNNON+PalatinoLinotype TrueType MacRoman yes yes no 112 0 CGNOEM+TimesNewRoman CID TrueType Identity-H yes yes yes 115 0 CGNPKF+TimesNewRoman TrueType WinAnsi yes yes no 121 0 CGNNJK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 130 0 CGNNJL+PalatinoLinotype CID TrueType Identity-H yes yes yes 133 0 CGNNJM+PalatinoLinotype TrueType WinAnsi yes yes no 139 0 CGNNJO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 CGNNON+PalatinoLinotype TrueType MacRoman yes yes no 145 0 CGNOEM+TimesNewRoman CID TrueType Identity-H yes yes yes 148 0 CGNPKF+TimesNewRoman TrueType WinAnsi yes yes no 154 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 160 0 CGNNJK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 171 0 CGNNJL+PalatinoLinotype CID TrueType Identity-H yes yes yes 174 0 CGNNJM+PalatinoLinotype TrueType WinAnsi yes yes no 180 0 CGNNJO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 183 0 CGNNON+PalatinoLinotype TrueType MacRoman yes yes no 186 0 CGNOEM+TimesNewRoman CID TrueType Identity-H yes yes yes 189 0 CGNNJK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 202 0 CGNNJL+PalatinoLinotype CID TrueType Identity-H yes yes yes 205 0 CGNNJM+PalatinoLinotype TrueType WinAnsi yes yes no 211 0 CGNNJO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 214 0 CGNNON+PalatinoLinotype TrueType MacRoman yes yes no 217 0 CGNOEM+TimesNewRoman CID TrueType Identity-H yes yes yes 220 0 CGNPKF+TimesNewRoman TrueType WinAnsi yes yes no 226 0 SYVAVM+VnURWPalladioL-Italic Type 1 Custom yes yes yes 242 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-17 02:57:39 CET RepresentationInformation: pmc_11505092.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-12-04 02:21:34 CET Size: 1243733 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 438 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals and Management Destination: subsection.2.1 Item: Title: Experimental Treatments Destination: subsection.2.2 Item: Title: Measurements, Sampling, and Analyses Destination: subsection.2.3 Children: Item: Title: Body Measurements Destination: subsubsection.2.3.1 Item: Title: Udder Health and Milk Composition Destination: subsubsection.2.3.2 Item: Title: Blood Samples Destination: subsubsection.2.3.3 Item: Title: Statistical Analyses Destination: subsection.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: Pre-Challenge Period Destination: subsection.3.1 Children: Item: Title: Thermophysiological Responses Destination: subsubsection.3.1.1 Item: Title: Lactation Performances Destination: subsubsection.3.1.2 Item: Title: Challenge Period Destination: subsection.3.2 Children: Item: Title: Thermophysiological Response Destination: subsubsection.3.2.1 Item: Title: Lactation Performance Destination: subsubsection.3.2.2 Item: Title: Immunity Responses Destination: subsubsection.3.2.3 Item: Title: Discussion Destination: section.4 Children: Item: Title: Pre-Challenge Period Destination: subsection.4.1 Item: Title: Challenge Period Destination: subsection.4.2 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Beta-Glucans Improve the Mammary Innate Immune Response to Endotoxin Challenge in Dairy Ewes Author: Santiago A. Guamán, Abdelaali Elhadi, Ahmed A. K. Salama, Carmen L. Manuelian, Gerardo Caja and Elena Albanell Subject: This study evaluated short-term immune responses of dairy ewes supplemented with barley -glucan (BG) following an intramammary Escherichia coli lipopolysaccharide (LPS) challenge. In the adaptation period, 36 ewes were fed an alfalfa hay diet ad libitum and barley grain cv. Hispanic (3.8% BG). Then, ewes were assigned into three experimental groups: (1) Control (CON), the same previous diet (13.3 g BG/d); (2) high -glucans barley (HBG), new barley (cv. Annapurna) containing 10% BG (35 g BG/d); (3) intraperitoneally injected (INP) with a 1.4% BG solution dose (2 g BG/ewe). At d 9, all ewes were infused with an E. coli LPS or saline solution in each udder half. After the challenge, rectal temperature (RT), milk yield and composition, somatic cell count (SCC), and plasma interleukins (IL-1 and IL-1) were monitored daily. The INP treatment revealed a transitory increase in RT and decreased milk yield by 38%. Milk fat, protein, and SCC increased in LPS-treated udders but not by BG treatment. The IL-1 plasma concentration was similar among groups but INP ewes showed a lower IL-1 concentration suggesting a lower inflammatory response. The BG administration appears more effective intraperitoneally than orally, which needs additional study. 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