a43dbe1566f04deeea71187238100473 pmc_11507496.pdf 17fa409695dce4d5b76f874f606725d312ee7c7f pmc_11507496.pdf 7977f030470a72b04cb6ce0a083efe1263e21a7df99ee7f7a33d5ab64a8f04ee pmc_11507496.pdf Title: Immunoaging at Early Ages Could Drive a Higher Comorbidity Burden in People with HIV on Antiretroviral Therapy Compared with the Uninfected Population Subject: This is an observational, cross-sectional, comparative case–control, pilot study aimed at assessing the impact of HIV infection and age on immunological markers in people with HIV (PWH) on antiretroviral therapy (ART). The study included 40 PWH on ART, divided into two age groups (40–45 years vs. 60 years), and 30 HIV-uninfected controls matched by sex and age. The results show that older PWH on ART had more comorbidities and a higher frequency of CD8 T cells compared to older controls, with a significant decrease in CD8 naïve T cells with age. Additionally, younger PWH on ART exhibited higher frequencies of activated CD8 T cells and elevated levels of inflammatory markers (sCD14, IL-6) compared to age-matched controls, with values similar to those of older PWH on ART. These findings suggest that younger PWH on ART may experience accelerated immunoaging, highlighting the need for early interventions in this population. Keywords: HIV; aging; inflammaging Author: Cora Loste, Macedonia Trigueros, Francisco Muñoz-López, Víctor Urrea, Ana Martínez, Sandra González, Jordi Puig, Marta Martín, Anna Bonjoch, Patricia Echeverría, Marta Massanella and Eugenia Negredo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Wed Oct 16 02:30:25 2024 CEST ModDate: Wed Oct 16 07:18:11 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1785293 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 FLAEEZ+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 YYIHQG+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 KHJVCQ+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 LFLYLN+CMSY10 Type 1 Builtin yes yes yes 32 0 HKBJPQ+VnURWPalladioL Type 1 Custom yes yes yes 37 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 53 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 56 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 73 0 PMFEMK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 90 0 PMFENM+PalatinoLinotype TrueType WinAnsi yes yes no 93 0 PMFENO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 96 0 PMFEMK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 119 0 PMFEML+PalatinoLinotype CID TrueType Identity-H yes yes yes 122 0 PMFENM+PalatinoLinotype TrueType WinAnsi yes yes no 128 0 PMFENO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 131 0 PMFFDN+PalatinoLinotype TrueType MacRoman yes yes no 134 0 PMFEMK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 159 0 PMFEML+PalatinoLinotype CID TrueType Identity-H yes yes yes 162 0 PMFENM+PalatinoLinotype TrueType WinAnsi yes yes no 168 0 PMFENO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 171 0 PMFFDN+PalatinoLinotype TrueType MacRoman yes yes no 174 0 PMFOFG+PalatinoLinotype,Italic TrueType MacRoman yes yes no 177 0 PMFEMK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 200 0 PMFEML+PalatinoLinotype CID TrueType Identity-H yes yes yes 203 0 PMFENM+PalatinoLinotype TrueType WinAnsi yes yes no 209 0 PMFENO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 212 0 PMFFDN+PalatinoLinotype TrueType MacRoman yes yes no 215 0 PMGCKM+PalatinoLinotype,Bold TrueType MacRoman yes yes no 218 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-06 02:44:31 CET RepresentationInformation: pmc_11507496.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-12-04 02:21:30 CET Size: 1785293 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 381 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: CD4 and CD8 T-Cell Distribution Destination: subsection.2.1 Item: Title: Senescence, Activation, and Exhaustion of T Cells Destination: subsection.2.2 Item: Title: Proinflammatory Status Destination: subsection.2.3 Item: Title: Contribution of HIV Infection and Age in Immunoaging Destination: subsection.2.4 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Study Design and Study Participants Destination: subsection.4.1 Item: Title: Study Variables Destination: subsection.4.2 Item: Title: Sample Processing Destination: subsection.4.3 Item: Title: CD4 and CD8 T Cell Immunophenotyping Destination: subsection.4.4 Item: Title: Quantification of Soluble Markers Destination: subsection.4.5 Item: Title: Statistical Analysis: Destination: subsection.4.6 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Immunoaging at Early Ages Could Drive a Higher Comorbidity Burden in People with HIV on Antiretroviral Therapy Compared with the Uninfected Population Author: Cora Loste, Macedonia Trigueros, Francisco Muñoz-López, Víctor Urrea, Ana Martínez, Sandra González, Jordi Puig, Marta Martín, Anna Bonjoch, Patricia Echeverría, Marta Massanella and Eugenia Negredo Subject: This is an observational, cross-sectional, comparative case control, pilot study aimed at assessing the impact of HIV infection and age on immunological markers in people with HIV (PWH) on antiretroviral therapy (ART). The study included 40 PWH on ART, divided into two age groups (40 45 years vs. 60 years), and 30 HIV-uninfected controls matched by sex and age. The results show that older PWH on ART had more comorbidities and a higher frequency of CD8 T cells compared to older controls, with a significant decrease in CD8 naïve T cells with age. Additionally, younger PWH on ART exhibited higher frequencies of activated CD8 T cells and elevated levels of inflammatory markers (sCD14, IL-6) compared to age-matched controls, with values similar to those of older PWH on ART. These findings suggest that younger PWH on ART may experience accelerated immunoaging, highlighting the need for early interventions in this population. 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