41fdac704e9a8c2e2ac8115e58e484a9 pmc_11510343.pdf 38e2f56974b04adb3bbc9dabce9911943666a9b9 pmc_11510343.pdf 19de7c4c7a545bc3e6f47f74fa8f0f3aa43a9b2f5758b43c9bcfe79ef291b0e4 pmc_11510343.pdf Title: Effect of Moderate Beer Intake on the Lipid Composition of Human Red Blood Cell Membranes Subject: Background/Objectives: Growing evidence suggests that erythrocyte membrane lipids are subject to changes during their lifespan. Factors such as the type of dietary intake and its composition contribute to the changes in red blood cell (RBC) membranes. Due to the high antioxidant content of beer, we aimed to investigate the effect of moderate beer consumption on the lipid composition of RBCs membranes from healthy overweight individuals. Methods: We conducted a four-weeks, prospective two-arm longitudinal crossed-over study, where participants (n = 36) were randomly assigned to alcohol-free beer group or traditional beer group. The lipids of RBCs membranes were assessed at the beginning and the end of the intervention by thin-layer chromatography. Results: Four-weeks of alcohol-free beer promoted changes in fatty acids (FA), free cholesterol (FC), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) (p < 0.05). Meanwhile, traditional beer intake led to changes in FA, FC, phospholipids (PL), PE and PC (p < 0.05). The observed alterations in membrane lipids were found to be independent of sex and BMI as influencing factors.Conclusions: The lipid composition of erythrocyte membranes is distinctly but mildly influenced by the consumption of both non-alcoholic and conventional beer, with no effects on RBCmembrane fluidity. Keywords: erythrocytes composition; phospholipids; fatty acids; cholesterol; alcohol consumption Author: Anallely López-Yerena, Natalia Muñoz-García, Victoria de Santisteban Villaplana, Teresa Padro and Lina Badimon Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Oct 18 15:41:04 2024 CEST ModDate: Fri Oct 18 15:45:13 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1968249 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 HPXFRG+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 DGDSBF+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 TBGJZF+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 RAFMMA+VnURWPalladioL Type 1 Custom yes yes yes 32 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 YFFKLH+CMSY10 Type 1 Builtin yes yes yes 68 0 JCLEKD+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 76 0 JCLDMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 79 0 JCLDNA+PalatinoLinotype CID TrueType Identity-H yes yes yes 82 0 JCLDNB+PalatinoLinotype TrueType WinAnsi yes yes no 88 0 JCLDND+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 91 0 JCLECD+PalatinoLinotype TrueType MacRoman yes yes no 94 0 JCLDMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 107 0 JCLDNA+PalatinoLinotype CID TrueType Identity-H yes yes yes 110 0 JCLDNB+PalatinoLinotype TrueType WinAnsi yes yes no 116 0 JCLDND+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 119 0 JCLECD+PalatinoLinotype TrueType MacRoman yes yes no 122 0 JCLDMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 129 0 JCLDNA+PalatinoLinotype CID TrueType Identity-H yes yes yes 132 0 JCLDNB+PalatinoLinotype TrueType WinAnsi yes yes no 138 0 JCLDNC+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 141 0 JCLDND+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 147 0 JCLECD+PalatinoLinotype TrueType MacRoman yes yes no 150 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 157 0 PDXFNG+PazoMath-Bold Type 1 Builtin yes yes yes 162 0 JCLDMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 173 0 JCLDNA+PalatinoLinotype CID TrueType Identity-H yes yes yes 176 0 JCLDNB+PalatinoLinotype TrueType WinAnsi yes yes no 182 0 JCLDND+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 185 0 JCLECD+PalatinoLinotype TrueType MacRoman yes yes no 188 0 MPUDFL+PazoMath Type 1 Builtin yes yes yes 195 0 JCLDMA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 203 0 JCLDNA+PalatinoLinotype CID TrueType Identity-H yes yes yes 206 0 JCLDNB+PalatinoLinotype TrueType WinAnsi yes yes no 212 0 JCLDND+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 215 0 JCLECD+PalatinoLinotype TrueType MacRoman yes yes no 218 0 WLOPFC+EURM10 Type 1 Builtin yes yes yes 225 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-06 02:46:52 CET RepresentationInformation: pmc_11510343.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-01-30 02:31:02 CET Size: 1968249 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 419 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Experimental Design and Study Population Destination: subsection.2.1 Item: Title: Ethical Statement Destination: subsection.2.2 Item: Title: Sample Collection Destination: subsection.2.3 Item: Title: Human Erythrocyte Ghosts’ Preparation Destination: subsection.2.4 Item: Title: Thin Layer Chromatography (TLC) Destination: subsection.2.5 Item: Title: Anthropometric Data, Blood Pressure, Serum Lipid Profile and Other Biochemical Measurements Destination: subsection.2.6 Item: Title: Statistical Analysis Destination: subsection.2.7 Item: Title: Results Destination: section.3 Children: Item: Title: RBC Membrane Lipid Composition at Baseline Destination: subsection.3.1 Item: Title: Effect of Beer Intake on RBC Membrane Lipid Composition Destination: subsection.3.2 Item: Title: Metabolic Remodeling of the Human Red Blood Cell Membrane Destination: subsection.3.3 Item: Title: Associations between Membrane Lipids and Circulating Lipids Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Effect of Moderate Beer Intake on the Lipid Composition of Human Red Blood Cell Membranes Author: Anallely López-Yerena, Natalia Muñoz-García, Victoria de Santisteban Villaplana, Teresa Padro and Lina Badimon Subject: Background/Objectives: Growing evidence suggests that erythrocyte membrane lipids are subject to changes during their lifespan. Factors such as the type of dietary intake and its composition contribute to the changes in red blood cell (RBC) membranes. Due to the high antioxidant content of beer, we aimed to investigate the effect of moderate beer consumption on the lipid composition of RBCs membranes from healthy overweight individuals. Methods: We conducted a four-weeks, prospective two-arm longitudinal crossed-over study, where participants (n = 36) were randomly assigned to alcohol-free beer group or traditional beer group. The lipids of RBCs membranes were assessed at the beginning and the end of the intervention by thin-layer chromatography. Results: Four-weeks of alcohol-free beer promoted changes in fatty acids (FA), free cholesterol (FC), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) (p < 0.05). Meanwhile, traditional beer intake led to changes in FA, FC, phospholipids (PL), PE and PC (p < 0.05). The observed alterations in membrane lipids were found to be independent of sex and BMI as influencing factors.Conclusions: The lipid composition of erythrocyte membranes is distinctly but mildly influenced by the consumption of both non-alcoholic and conventional beer, with no effects on RBCmembrane fluidity. 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