a327c3d3412eb49ad00ad9129dc9b5eb pmc_11511405.pdf 6d68fa0946029a1d69281e3d59eb2250b454e5e8 pmc_11511405.pdf 730811f59fee9fe49461ed88454ed61f167d0a6645ee91266a91bf784a5b8e10 pmc_11511405.pdf Title: Immunohistochemical Expression of Tenascin-C in Canine Meningiomas Subject: In humans, tenascin-C (TN-C) expression has been detected in more aggressive neoplasms of the central nervous system, such as gliomas and meningiomas. No study has analyzed the immune expression of TN-C in canine meningioma. The current study aimed to investigate the immunohistochemical distribution of TN-C in different grades of canine meningiomas. Twenty-one cases of canine meningioma (12 grade I, 6 grade II, and 3 grade III) were analyzed. All samples were examined by immunohistochemistry with the following antibodies: TN-C, epithelial membrane antigen (EMA), Ki-67, pan-cytokeratin (Pan CK), and vimentin. The histopathological diagnosis of meningioma was reinforced with the positive labeling of vimentin (moderate to strong) and EMA (mild to moderate) in neoplastic cells in most cases, independently of its grade or subtype. The immunoreactivity of TN-C was irregular: mild in grade I, moderate in grade II, and moderate to severe in grade III neoplasms. Usually, immune positivity was observed in the stroma and perivascular space in all subtypes. In addition, the concentric whorls of neoplastic cells were labeled positive in some psammomatous and transitional meningiomas. The reaction to TN-C was more significant in grade II and III tumors. The immunohistochemical findings of the current study suggest that TN-C can act as a stromal marker, mainly in grade II or III meningiomas. Keywords: neuropathology; meningioma; oncology; TN-C Author: Fabiano José Ferreira de Sant’Ana, Ester Blasco and Martí Pumarola Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Tue Oct 8 11:42:32 2024 CEST ModDate: Tue Oct 8 11:45:44 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3778018 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 GFKJOY+VnURWPalladioL Type 1 Custom yes yes yes 16 0 OMUOHM+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 NFPDZU+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 EQXQPH+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 IXLVLQ+CMSY10 Type 1 Builtin yes yes yes 65 0 BDGJNL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 82 0 BDGJNM+PalatinoLinotype CID TrueType Identity-H yes yes yes 85 0 BDGJNN+PalatinoLinotype TrueType WinAnsi yes yes no 91 0 BDGJNP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 94 0 BDGJNL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 104 0 BDGJNM+PalatinoLinotype CID TrueType Identity-H yes yes yes 107 0 BDGJNN+PalatinoLinotype TrueType WinAnsi yes yes no 113 0 BDGJNP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 116 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-17 02:57:38 CET RepresentationInformation: pmc_11511405.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-12-04 02:21:29 CET Size: 3778018 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 263 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: section.5 Info: Title: Immunohistochemical Expression of Tenascin-C in Canine Meningiomas Author: Fabiano José Ferreira de Sant’Ana, Ester Blasco and Martí Pumarola Subject: In humans, tenascin-C (TN-C) expression has been detected in more aggressive neoplasms of the central nervous system, such as gliomas and meningiomas. No study has analyzed the immune expression of TN-C in canine meningioma. The current study aimed to investigate the immunohistochemical distribution of TN-C in different grades of canine meningiomas. Twenty-one cases of canine meningioma (12 grade I, 6 grade II, and 3 grade III) were analyzed. All samples were examined by immunohistochemistry with the following antibodies: TN-C, epithelial membrane antigen (EMA), Ki-67, pan-cytokeratin (Pan CK), and vimentin. The histopathological diagnosis of meningioma was reinforced with the positive labeling of vimentin (moderate to strong) and EMA (mild to moderate) in neoplastic cells in most cases, independently of its grade or subtype. The immunoreactivity of TN-C was irregular: mild in grade I, moderate in grade II, and moderate to severe in grade III neoplasms. Usually, immune positivity was observed in the stroma and perivascular space in all subtypes. In addition, the concentric whorls of neoplastic cells were labeled positive in some psammomatous and transitional meningiomas. The reaction to TN-C was more significant in grade II and III tumors. The immunohistochemical findings of the current study suggest that TN-C can act as a stromal marker, mainly in grade II or III meningiomas. 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