096337705ca2f6b1f0942ac07e86e9f5 pmc_11591060.pdf 3a8d009c6b9f67e3a42bbe9efda012f6c7abe718 pmc_11591060.pdf 8c08da0c8629f422956dd1dccee42a9652d320998ea858c95a46a4cd887b2ef9 pmc_11591060.pdf Title: Long-Term Effects of Early Low-Phosphorous Nutritional Conditioning on Broiler Chicken Performance, Bone Mineralization, and Gut Health Under Adequate or Phosphorous-Deficient Diets Subject: Phosphorous is essential for many metabolic functions and the constitution of bones. Poultry have limited ability to use phosphorous from diets, which is mainly excreted and causes environmental concern. For this reason, diets are commonly supplemented with inorganic phosphorus and phytases. It has been suggested that chickens can adapt to an early nutrient restriction by increasing its efficiency of utilization, an adaptation that has been defined as nutritional conditioning. The aim of this study was to investigate a strategy of phosphorous nutritional conditioning by feeding low phosphorous diets during the first week of life as a strategy to improve the efficiency of phosphorous utilization later in life. To test this, 600 male broiler chickens were fed either a standard (control group) or a phosphorous-deficient diet (conditioned group) during the first week of life. Later in life, the effect of conditioning was tested using standard or P-deficient diets during the finishing phase (21–43 d). Conditioning did not affect overall performance, despite finding evidence for reduced relative phosphorous excretion between days 19 to 21, increased duodenal gene expression for the phosphorous transporter SLC34A2 at day 30 (-6% and +17%, respectively), and tendencies for improved phosphorous digestibility (+7%) and tibia mineralization (+6%) at the end of the trial. It is concluded that phosphorous nutritional conditioning early in life can increase subsequent dietary phosphorous utilization and bone mineralization in poultry, although it may not be able to counteract severe phosphorous deficiencies. Further research is required to assess the extent to which phosphorous supplementation in post-conditioning diets may be reduced with this strategy without compromising performance. Keywords: chicken; phosphorous restriction; calcium and phosphorus digestibility; phosphorous efficiency; bone mineralization Author: Núria Tous, Maria Francesch, Joan Tarradas, Ignacio Badiola, Ana M. Pérez de Rozas, Emma Fàbrega, Maria Ballester, Raquel Quintanilla and David Torrallardona Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Sat Nov 9 09:43:03 2024 CET ModDate: Sat Nov 9 09:45:43 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 295409 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AALFUC+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 BFLCUP+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 TOQAGD+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 UYDSJL+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 CLDDOF+CMSY10 Type 1 Builtin yes yes yes 32 0 YIXCPV+VnURWPalladioL Type 1 Custom yes yes yes 37 0 LEVXPH+EURM10 Type 1 Builtin yes yes yes 73 0 MPUDFL+PazoMath Type 1 Builtin yes yes yes 84 0 EQZEBA+URWPalladioL-BoldItal Type 1 Custom yes yes yes 92 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-27 02:09:59 CET RepresentationInformation: pmc_11591060.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-27 02:03:21 CET Size: 295409 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 260 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals, Housing, and Diets Destination: subsection.2.1 Item: Title: Growth Performance and Gait Score in Group Housed Animals Destination: subsection.2.2 Item: Title: Apparent Total Tract and Apparent Prececal Digestibility Destination: subsection.2.3 Item: Title: Bone Mineralization Destination: subsection.2.4 Item: Title: Cotransporter SLC34A2 Expression Destination: subsection.2.5 Item: Title: Morphometry of Intestinal Mucosa and Gut Immunology Destination: subsection.2.6 Item: Title: Statistical Analysis Destination: subsection.2.7 Item: Title: Results Destination: section.3 Children: Item: Title: Performance Destination: subsection.3.1 Item: Title: Apparent Prececal Digestibility, Bone Mineralization, and Gait Score Destination: subsection.3.2 Item: Title: Apparent Total Tract Digestibility and Nutrient Excretion Destination: subsection.3.3 Item: Title: Gut Expression of SLC34A2 Destination: subsection.3.4 Item: Title: Histology and Immunological Traits of Broiler Chickens Destination: subsection.3.5 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Long-Term Effects of Early Low-Phosphorous Nutritional Conditioning on Broiler Chicken Performance, Bone Mineralization, and Gut Health Under Adequate or Phosphorous-Deficient Diets Author: Núria Tous, Maria Francesch, Joan Tarradas, Ignacio Badiola, Ana M. Pérez de Rozas, Emma Fàbrega, Maria Ballester, Raquel Quintanilla and David Torrallardona Subject: Phosphorous is essential for many metabolic functions and the constitution of bones. Poultry have limited ability to use phosphorous from diets, which is mainly excreted and causes environmental concern. For this reason, diets are commonly supplemented with inorganic phosphorus and phytases. It has been suggested that chickens can adapt to an early nutrient restriction by increasing its efficiency of utilization, an adaptation that has been defined as nutritional conditioning. The aim of this study was to investigate a strategy of phosphorous nutritional conditioning by feeding low phosphorous diets during the first week of life as a strategy to improve the efficiency of phosphorous utilization later in life. To test this, 600 male broiler chickens were fed either a standard (control group) or a phosphorous-deficient diet (conditioned group) during the first week of life. Later in life, the effect of conditioning was tested using standard or P-deficient diets during the finishing phase (21 43 d). Conditioning did not affect overall performance, despite finding evidence for reduced relative phosphorous excretion between days 19 to 21, increased duodenal gene expression for the phosphorous transporter SLC34A2 at day 30 (-6% and +17%, respectively), and tendencies for improved phosphorous digestibility (+7%) and tibia mineralization (+6%) at the end of the trial. It is concluded that phosphorous nutritional conditioning early in life can increase subsequent dietary phosphorous utilization and bone mineralization in poultry, although it may not be able to counteract severe phosphorous deficiencies. Further research is required to assess the extent to which phosphorous supplementation in post-conditioning diets may be reduced with this strategy without compromising performance. 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