fba03c8913393c993006483dcff86dc2 pmc_11598865.pdf 13ef5052324768ede57744446757fe26eb5b0807 pmc_11598865.pdf 02b414a48888fb43cd53e19e0daafd5611babfc6cd1785cec065401f0617005b pmc_11598865.pdf Title: Short Term Treatment Monitoring of Renal and Inflammatory Biomarkers with Naturally Occurring Leishmaniosis: A Cohort Study of 30 Dogs Subject: Various inflammatory and renal biomarkers have already been assessed for monitoring the response to anti-leishmanial therapy in canine leishmaniosis. This study assessed the parasite load, various inflammatory and renal biomarkers pre- and post-treatment, and any association between the studied variables and the degree of disease severity at diagnosis. This is a prospective cohort study of 30 client-owned dogs with leishmaniosis, classified according to LeishVet’s guidelines as stage I (n = 2), stage IIa (n = 7), stage IIb (n = 6), stage III (n = 8), and stage IV (n = 7). In addition to Leishmania real-time PCR in the bone marrow, blood and urine, previously studied biomarkers, and several inflammatory and renal markers never investigated in canine leishmaniosis, such as fibrinogen, antithrombin, urinary fractional excretion of sodium, and urinary amylase-to-creatinine ratio were measured pre- and post-treatment (meglumine antimoniate or miltefosine + allopurinol). A positive Leishmania real-time PCR in the blood at diagnosis predicted a positive Leishmania real-time PCR in the bone marrow post-treatment (p = 0.003). Following treatment, antithrombin and urinary amylase-to-creatinine ratio were significantly changed (p < 0.001, respectively). Urinary amylase-to-creatinine ratio, total iron-binding capacity, and antithrombin were the variables most strongly associated with disease severity (p < 0.005, respectively). Urinary amylase-to-creatinine ratio can be a useful marker to monitor treatment response and to classify the degree of disease severity. Keywords: antithrombin; C-reactive protein; Leishmania infantum; severity; total iron-binding capacity; urinary amylase-to-creatinine ratio Author: Valeria Pantaleo, Tommaso Furlanello, Michela Campigli, Laura Ventura and Laia Solano-Gallego Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Tue Oct 22 07:27:10 2024 CEST ModDate: Tue Oct 22 07:31:34 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 650096 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FBYTTV+VnURWPalladioL Type 1 Custom yes yes yes 10 0 VMOAED+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 EYABGA+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 WSHUKH+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 43 0 MDJLZU+EURM10 Type 1 Builtin yes yes yes 63 0 XEYJZU+CMSY10 Type 1 Builtin yes yes yes 71 0 OAEABB+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 85 0 OAEABC+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 91 0 OAEAEC+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 97 0 OAEAED+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 103 0 OAEAEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 106 0 OAEAEF+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 109 0 OAEAFH+PalatinoLinotype-Roman TrueType MacRoman yes yes no 112 0 OAEBIB+PalatinoLinotype-Italic TrueType MacRoman yes yes no 115 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 123 0 EUQOAW+EURM7 Type 1 Builtin yes yes yes 134 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-12-17 02:57:31 CET RepresentationInformation: pmc_11598865.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-12-04 02:21:17 CET Size: 650096 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 432 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Dogs and Study Design Destination: subsection.2.1 Item: Title: Treatment Protocol and Follow-Up Destination: subsection.2.2 Item: Title: Sample Collection and Diagnostic Tests Destination: subsection.2.3 Item: Title: Sample Size Destination: subsection.2.4 Item: Title: Statistical Methods Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Clinical, Serological, and Molecular Data as Well as Individual Data at the Time of Diagnosis and Post-Treatment Destination: subsection.3.1 Item: Title: Relationship Between Molecular, Inflammatory, and Renal Markers at the Time Diagnosis and Post-Treatment Destination: subsection.3.2 Item: Title: Evaluation of Molecular, Inflammatory and Renal Markers Based on Disease Severity Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Short Term Treatment Monitoring of Renal and Inflammatory Biomarkers with Naturally Occurring Leishmaniosis: A Cohort Study of 30 Dogs Author: Valeria Pantaleo, Tommaso Furlanello, Michela Campigli, Laura Ventura and Laia Solano-Gallego Subject: Various inflammatory and renal biomarkers have already been assessed for monitoring the response to anti-leishmanial therapy in canine leishmaniosis. This study assessed the parasite load, various inflammatory and renal biomarkers pre- and post-treatment, and any association between the studied variables and the degree of disease severity at diagnosis. This is a prospective cohort study of 30 client-owned dogs with leishmaniosis, classified according to LeishVet’s guidelines as stage I (n = 2), stage IIa (n = 7), stage IIb (n = 6), stage III (n = 8), and stage IV (n = 7). In addition to Leishmania real-time PCR in the bone marrow, blood and urine, previously studied biomarkers, and several inflammatory and renal markers never investigated in canine leishmaniosis, such as fibrinogen, antithrombin, urinary fractional excretion of sodium, and urinary amylase-to-creatinine ratio were measured pre- and post-treatment (meglumine antimoniate or miltefosine + allopurinol). A positive Leishmania real-time PCR in the blood at diagnosis predicted a positive Leishmania real-time PCR in the bone marrow post-treatment (p = 0.003). Following treatment, antithrombin and urinary amylase-to-creatinine ratio were significantly changed (p < 0.001, respectively). Urinary amylase-to-creatinine ratio, total iron-binding capacity, and antithrombin were the variables most strongly associated with disease severity (p < 0.005, respectively). Urinary amylase-to-creatinine ratio can be a useful marker to monitor treatment response and to classify the degree of disease severity. 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