d1cd4b78b32d0f8233be9d60010fddf2 pmc_11680271.pdf 029d8f05b47861cbcd567ae0b7c4d4cbab5057f6 pmc_11680271.pdf fe571a893ae83e201297ac078b1b2dc12d5acf7c267f3fde37e1a20fd57b6c3a pmc_11680271.pdf Title: The Use of the Nitroblue Tetrazolium Test in Blood Granulocytes for Discriminating Bacterial and Non-Bacterial Neutrophilic Dermatitis Subject: This study aimed to evaluate differences in the production of reactive oxygen species (ROS) by peripheral blood neutrophils in healthy dogs, dogs with superficial pyoderma, and dogs with sterile neutrophilic dermatitis using the nitroblue tetrazolium (NBT) reduction test. Additionally, the study assessed the potential of the NBT reduction test as a diagnostic tool to differentiate between these clinical conditions. A total of 28 dogs were divided into three groups: healthy (n = 10), superficial pyoderma (n = 10), and sterile neutrophilic dermatitis (n = 8). The results showed significantly higher neutrophil activation in dogs with sterile neutrophilic dermatitis (19 10%) compared with healthy dogs (7.3 5%) (p < 0.01) and dogs with superficial pyoderma (10 2.8%) (p < 0.05). Dogs with sterile neutrophilic dermatitis and a high positive result for Leishmania spp. antibodies (n = 3) exhibited even higher NBT reduction rates (30 4.6%) compared with seronegative dogs with sterile neutrophilic dermatitis (n = 5) (13 5.3%) (p < 0.05). These findings suggest that the NBT reduction test could be useful for detecting systemic neutrophil activation in sterile neutrophilic dermatitis, especially when associated with Leishmania infection. However, no significant differences were observed between healthy dogs and those with superficial pyoderma, indicating that the test may not be effective in detecting systemic neutrophil activation in superficial bacterial infections. Keywords: NBT; dog; neutrophil; superficial pyoderma; sterile neutrophilic dermatitis; oxidative metabolism Author: Marina García, Icíar Martínez-Flórez, Laia Solano-Gallego, Nuria García and Laura Ordeix Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Dec 9 18:10:56 2024 CET ModDate: Mon Dec 9 18:55:20 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 706527 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 OZFFOV+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 PLRRTC+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 WSIKJZ+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 UWJUYN+CMSY10 Type 1 Builtin yes yes yes 32 0 CIQLLF+VnURWPalladioL Type 1 Custom yes yes yes 37 0 OQIVQT+EURM10 Type 1 Builtin yes yes yes 70 0 CNHKME+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 78 0 CNHKMF+PalatinoLinotype CID TrueType Identity-H yes yes yes 81 0 CNHKNG+PalatinoLinotype TrueType WinAnsi yes yes no 87 0 CNHKNI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 90 0 CNHLBI+PalatinoLinotype TrueType MacRoman yes yes no 93 0 CNHKME+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 106 0 CNHKMF+PalatinoLinotype CID TrueType Identity-H yes yes yes 109 0 CNHKNG+PalatinoLinotype TrueType WinAnsi yes yes no 115 0 CNHKNI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 118 0 CNHLBI+PalatinoLinotype TrueType MacRoman yes yes no 121 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-15 02:46:07 CET RepresentationInformation: pmc_11680271.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-12 02:33:05 CET Size: 706527 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 234 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Animals Destination: subsection.2.1 Item: Title: Ethics Destination: subsection.2.2 Item: Title: Blood Collection Destination: subsection.2.3 Item: Title: Blood Nitroblue Tetrazolium Reduction Test Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: The Use of the Nitroblue Tetrazolium Test in Blood Granulocytes for Discriminating Bacterial and Non-Bacterial Neutrophilic Dermatitis Author: Marina García, Icíar Martínez-Flórez, Laia Solano-Gallego, Nuria García and Laura Ordeix Subject: This study aimed to evaluate differences in the production of reactive oxygen species (ROS) by peripheral blood neutrophils in healthy dogs, dogs with superficial pyoderma, and dogs with sterile neutrophilic dermatitis using the nitroblue tetrazolium (NBT) reduction test. Additionally, the study assessed the potential of the NBT reduction test as a diagnostic tool to differentiate between these clinical conditions. A total of 28 dogs were divided into three groups: healthy (n = 10), superficial pyoderma (n = 10), and sterile neutrophilic dermatitis (n = 8). The results showed significantly higher neutrophil activation in dogs with sterile neutrophilic dermatitis (19 10%) compared with healthy dogs (7.3 5%) (p < 0.01) and dogs with superficial pyoderma (10 2.8%) (p < 0.05). Dogs with sterile neutrophilic dermatitis and a high positive result for Leishmania spp. antibodies (n = 3) exhibited even higher NBT reduction rates (30 4.6%) compared with seronegative dogs with sterile neutrophilic dermatitis (n = 5) (13 5.3%) (p < 0.05). These findings suggest that the NBT reduction test could be useful for detecting systemic neutrophil activation in sterile neutrophilic dermatitis, especially when associated with Leishmania infection. However, no significant differences were observed between healthy dogs and those with superficial pyoderma, indicating that the test may not be effective in detecting systemic neutrophil activation in superficial bacterial infections. 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