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Language: en Outlines: Item: Title: Low-cost inkjet-printed nanostructured biosensor based on CRISPR/Cas12a system for pathogen detection Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Materials and reagents Destination: mk:H2_1 Item: Title: 2.2 Fabrication of the inkjet-printed gold electrodes Destination: mk:H2_2 Item: Title: 2.3 Bacterial strains and genomic DNA extraction Destination: mk:H2_3 Item: Title: 2.4 Gene selection and amplification Destination: mk:H2_4 Item: Title: 2.5 Oligonucleotides selection and design Destination: mk:H2_5 Children: Item: Title: 2.5.1 ssDNA-MB reporter design Destination: mk:H3_6 Item: Title: 2.5.2 Design of the guide RNA (gRNA) Destination: mk:H3_7 Item: Title: 2.6 Operative parameters Destination: mk:H2_8 Children: Item: Title: 2.6.1 Electrode cleaning and functionalization Destination: mk:H3_9 Item: Title: 2.6.2 Probe surface density calculation Destination: mk:H3_10 Item: Title: 2.6.3 Cas12a/gRNA complex assembly and activation Destination: mk:H3_11 Item: Title: 2.6.4 Square wave voltammetry Cas12a/gRNA investigation Destination: mk:H3_12 Item: Title: 3 Results and discussion Destination: mk:H1_3 Children: Item: Title: 3.1 Assay biosensing principle Destination: mk:H2_13 Item: Title: 3.2 Biosensor characterization Destination: mk:H2_14 Children: Item: Title: 3.2.1 Cleaning Destination: mk:H3_15 Item: Title: 3.2.2 Functionalization of the electrodes Destination: mk:H3_16 Item: Title: 3.2.3 Probe density calculation Destination: mk:H3_17 Item: Title: 3.3 Cas12a gRNA biosensing assay performances Destination: mk:H2_18 Children: Item: Title: 3.3.1 Performances of different sensors for DNA detection Destination: mk:H3_19 Item: Title: 3.3.2 Detection of clinical isolates Destination: mk:H3_20 Item: Title: 4 Conclusions Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_4 Item: Title: Data availability Destination: datalink5 Item: Title: Acknowledgements Destination: mk:HH1_6 Item: Title: Appendix A Supplementary data Destination: mk:H1_6 Item: Title: References Destination: mk:HH2_1 Info: Title: Low-cost inkjet-printed nanostructured biosensor based on CRISPR/Cas12a system for pathogen detection Author: Angela Gilda Carota Subject: Biosensors and Bioelectronics, 258 (2024) 116340. doi:10.1016/j.bios.2024.116340 Keywords: CRISPR/Cas12a,Electrochemical biosensing platform,Inkjet-printed electrodes,Pathogenic bacteria Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Sun May 12 07:40:43 CEST 2024 ModDate: Mon May 13 09:52:41 CEST 2024 ID: 0xacd96df00ac5b55e816b8ed29030c8d8, 0xd3f82e713bf357ee1673179623427876 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG 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Biosensors and Bioelectronics, 258 (2024) 116340. doi:10.1016/j.bios.2024.116340 CRISPR/Cas12a Electrochemical biosensing platform Inkjet-printed electrodes Pathogenic bacteria Low-cost inkjet-printed nanostructured biosensor based on CRISPR/Cas12a system for pathogen detection Angela Gilda Carota Andrea Bonini Massimo Urban Noemi Poma Federico Maria Vivaldi Arianna Tavanti Marianna Rossetti Giulio Rosati Arben Merkoçi Fabio Di Francesco journal Biosensors and Bioelectronics © 2024 The Authors. Published by Elsevier B.V. 0956-5663 258 15 August 2024 2024-08-15 116340 116340 10.1016/j.bios.2024.116340 https://doi.org/10.1016/j.bios.2024.116340 http://creativecommons.org/licenses/by-nc-nd/4.0/ VoR 10.1016/j.bios.2024.116340 noindex 13th May 2024 Elsevier 2024-05-13T07:52:41 2024-05-12T05:40:43 2024-05-13T07:52:41 True 13th May 2024 Acrobat Distiller 8.1.0 (Windows) CRISPR/Cas12a,Electrochemical biosensing platform,Inkjet-printed electrodes,Pathogenic bacteria uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 1 https://www.elsevier.com/tdm/tdmrep-policy.json Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 134, 576, 220, 587 Flags: Print Destination: 978 Annotation: Subtype: Link Rect: 221, 576, 228, 587 Flags: Print Destination: 978 Annotation: Subtype: Link Rect: 228, 576, 235, 587 Flags: Print Destination: 978 Annotation: Subtype: Link Rect: 236, 579, 240, 584 Flags: Print Destination: null Annotation: Subtype: Link Rect: 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