b5f30f7cb1c970a18fb034554bfe5ef8 microplastics-04-00001.pdf 18620c774c2a1446684f33da3d329d5c1f0f5101 microplastics-04-00001.pdf 39be8fa3b82281b67f8b7dd5305a8a3fdff639f8023a4527d1094052a8767d30 microplastics-04-00001.pdf Title: Prospective Investigation of Nanoplastic Accumulation in Healthy Subjects, Autoimmune Diseases, Hematological Malignancies, Lung Cancer, and Murine Models Subject: Nanoplastics (NPs) and microplastics (MPs) are an emerging threat to global health. They negatively impact ecosystems and many physiological processes, causing alterations in xenobiotic metabolism, nutrient uptake, energy metabolism, or cytotoxicity. In humans, we are beginning to analyze these plastics for the mechanisms by which they enter the organism, accumulate, and diffuse as well as for their pathogenic potential. NP accumulation has been demonstrated in human tissues, such as blood or placenta, while in others it remains largely unstudied. In this work, we detected NP accumulation in bronchoalveolar lavage fluids (BALFs), cerebrospinal fluids (CSFs), lymph nodes (LNs), urine, pleural fluids (PFs), ascitic fluids (AFs), and peripheral blood (PB) by combining fluorescence and nanocytometry techniques. NP analysis was compared with two strains of mice, and the results support that inhalation is the main route of NP accumulation. Keywords: nanoplastics; detection; accumulation; human; mice; flow cytometry;nanocytometry Author: Roser Salvia, Manel Cañaveras, Laura G. Rico, Ana Drozdowskyj, Michael D. Ward, Rebeca Jurado, Laia Gómez-Muñoz, Marta Vives-Pi, Eva Martínez-Cáceres and Jordi Petriz Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Dec 30 05:37:10 2024 CET ModDate: Mon Dec 30 05:41:41 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1991567 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 BKSAQO+URWPalladioL-Roma Type 1 Custom yes yes yes 16 0 BBLSUK+URWPalladioL-Bold Type 1 Custom yes yes yes 22 0 DZUQHD+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 EIHCIX+VnURWPalladioL Type 1 Custom yes yes yes 32 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 62 0 MDJHIH+CMSY10 Type 1 Builtin yes yes yes 70 0 HAGFPJ+PalatinoLinotype,Bold 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yes yes no 344 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-01-15 02:41:15 CET RepresentationInformation: microplastics-04-00001.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-01-09 17:18:57 CET Size: 1991567 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 503 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Material and Methods Destination: section.2 Children: Item: Title: Human Samples Destination: subsection.2.1 Item: Title: Mice Destination: subsection.2.2 Item: Title: Sample Preparation and Flow Cytometry Destination: subsection.2.3 Item: Title: Quality Control Destination: subsection.2.4 Item: Title: Ethics Destination: subsection.2.5 Item: Title: Statistics Destination: subsection.2.6 Item: Title: Results and Discussion Destination: section.3 Item: Title: References Destination: appendix.A. Info: Title: Prospective Investigation of Nanoplastic Accumulation in Healthy Subjects, Autoimmune Diseases, Hematological Malignancies, Lung Cancer, and Murine Models Author: Roser Salvia, Manel Cañaveras, Laura G. Rico, Ana Drozdowskyj, Michael D. Ward, Rebeca Jurado, Laia Gómez-Muñoz, Marta Vives-Pi, Eva Martínez-Cáceres and Jordi Petriz Subject: Nanoplastics (NPs) and microplastics (MPs) are an emerging threat to global health. They negatively impact ecosystems and many physiological processes, causing alterations in xenobiotic metabolism, nutrient uptake, energy metabolism, or cytotoxicity. In humans, we are beginning to analyze these plastics for the mechanisms by which they enter the organism, accumulate, and diffuse as well as for their pathogenic potential. NP accumulation has been demonstrated in human tissues, such as blood or placenta, while in others it remains largely unstudied. In this work, we detected NP accumulation in bronchoalveolar lavage fluids (BALFs), cerebrospinal fluids (CSFs), lymph nodes (LNs), urine, pleural fluids (PFs), ascitic fluids (AFs), and peripheral blood (PB) by combining fluorescence and nanocytometry techniques. NP analysis was compared with two strains of mice, and the results support that inhalation is the main route of NP accumulation. 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