f8dc5650dedacd1865981ca060a30ee8 PAT_a2025v14n3m3p246.pdf 57358e9655055a05d2cdd5f5c8ef972e58943441 PAT_a2025v14n3m3p246.pdf cdcfb5fe38504c10ca01e975db821c45b1f83bc23c134e5c5f410f5673151390 PAT_a2025v14n3m3p246.pdf Title: Clinical, Immunological and Pathological Characteristics of Ischemic Dermatopathy in Dogs with Leishmaniosis Subject: Cutaneous lesions suggestive of vasculitis and/or ischemic dermatopathy (ID) are anecdotally reported in canine leishmaniosis, and the clinicopathological features of these conditions have not been fully characterized. The objective of this case series was to describe six dogs with leishmaniosis and ID. In 5/6 dogs, leishmaniosis was diagnosed at the time of ID diagnosis, whereas in 1/6 dogs, ID developed during the first month of anti-Leishmania conventional treatment. One each of greyhound, Chihuahua, whippet, American bully, hound and mixed breeds were represented, and the median age at presentation was 6 years [2–8]. All patients presented high or very high levels of circulating anti-Leishmania infantum antibodies. The cutaneous lesions were multifocal alopecia with atrophic skin with hyper- or hypopigmentation (6/6), ulcers located on the extremities and trunk (3/6) and onychodystrophy (2/6). Histologically, ID was confirmed by the presence of follicular atrophy (faded follicles) (6/6), perivascular or interstitial lymphoplasmacytic dermatitis or panniculitis (6/6), collagen smudging (3/6), dermal fibrosis (3/6), lymphocytic interface dermatitis (3/6) and ulceration (3/6). Vasculopathy was observed in the superficial and mid-vascular plexuses in 4/6 dogs and characterized by the combination of some of the following lesions: vasocongestion, hemorrhagic foci, mild hyaline mural degeneration, thrombi and fragmented degenerating nuclear debris of neutrophils in the vascular wall. Moreover, myositis was observed in 1/6 cases. Leishmania-specific immunohistochemistry was positive in the skin of 4/6 cases. Leishmaniosis might be considered an underlying cause of ID in dogs. However, the immune mechanisms and pathogenesis need to be elucidated. Keywords: canine; Leishmania infantum; diagnosis; vasculopathy; antibodies Author: Nuria García, Àlex Cobos, Laia Solano-Gallego, Marina García and Laura Ordeix Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Tue Mar 4 11:21:15 2025 CET ModDate: Tue Mar 4 11:26:38 2025 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 10767779 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SSXPJJ+VnURWPalladioL Type 1 Custom yes yes yes 10 0 DVEFCD+URWPalladioL-Roma Type 1 Custom yes yes yes 16 0 IFECSQ+URWPalladioL-Bold Type 1 Custom yes yes yes 22 0 SFKSEM+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 IXLVLQ+CMSY10 Type 1 Builtin yes yes yes 56 0 FLKGOO+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 67 0 FLKGOP+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 73 0 FLKHAA+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 79 0 FLKHAB+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 85 0 FLKHAC+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 88 0 FLKHAD+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 91 0 FLKHGC+PalatinoLinotype-Roman TrueType MacRoman yes yes no 94 0 FLKGOO+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 110 0 FLKGOP+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 116 0 FLKHAA+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 122 0 FLKHAB+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 128 0 FLKHAC+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 131 0 FLKHAD+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 134 0 FLKGOO+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 145 0 FLKGOP+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 151 0 FLKHAA+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 157 0 FLKHAB+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 163 0 FLKHAC+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 166 0 FLKHAD+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 169 0 FLKHGC+PalatinoLinotype-Roman TrueType MacRoman yes yes no 172 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-04-12 02:41:08 CEST RepresentationInformation: PAT_a2025v14n3m3p246.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-04-11 09:42:59 CEST Size: 10767779 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 291 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Case Selection Destination: subsection.2.1 Item: Title: Data and Sample Collection Destination: subsection.2.2 Item: Title: Histopathology Evaluation Destination: subsection.2.3 Item: Title: Leishmania Infantum Immunohistochemistry Destination: subsection.2.4 Item: Title: IgG Immunohistochemistry Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Clinicopathological Data and Treatment Response Destination: subsection.3.1 Item: Title: Histopathological Evaluation Destination: subsection.3.2 Item: Title: Histochemical and Immunohistochemical Evaluation Destination: subsection.3.3 Item: Title: Leishmania spp. Immunohistochemistry Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Clinical, Immunological and Pathological Characteristics of Ischemic Dermatopathy in Dogs with Leishmaniosis Author: Nuria García, Àlex Cobos, Laia Solano-Gallego, Marina García and Laura Ordeix Subject: Cutaneous lesions suggestive of vasculitis and/or ischemic dermatopathy (ID) are anecdotally reported in canine leishmaniosis, and the clinicopathological features of these conditions have not been fully characterized. The objective of this case series was to describe six dogs with leishmaniosis and ID. In 5/6 dogs, leishmaniosis was diagnosed at the time of ID diagnosis, whereas in 1/6 dogs, ID developed during the first month of anti-Leishmania conventional treatment. One each of greyhound, Chihuahua, whippet, American bully, hound and mixed breeds were represented, and the median age at presentation was 6 years [2 8]. All patients presented high or very high levels of circulating anti-Leishmania infantum antibodies. The cutaneous lesions were multifocal alopecia with atrophic skin with hyper- or hypopigmentation (6/6), ulcers located on the extremities and trunk (3/6) and onychodystrophy (2/6). Histologically, ID was confirmed by the presence of follicular atrophy (faded follicles) (6/6), perivascular or interstitial lymphoplasmacytic dermatitis or panniculitis (6/6), collagen smudging (3/6), dermal fibrosis (3/6), lymphocytic interface dermatitis (3/6) and ulceration (3/6). Vasculopathy was observed in the superficial and mid-vascular plexuses in 4/6 dogs and characterized by the combination of some of the following lesions: vasocongestion, hemorrhagic foci, mild hyaline mural degeneration, thrombi and fragmented degenerating nuclear debris of neutrophils in the vascular wall. Moreover, myositis was observed in 1/6 cases. Leishmania-specific immunohistochemistry was positive in the skin of 4/6 cases. Leishmaniosis might be considered an underlying cause of ID in dogs. However, the immune mechanisms and pathogenesis need to be elucidated. 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