f6c1760815e28bb1d07a4192534cfa4c agronomy-15-00287-v2.pdf e501f3516e063b66fbe3d5afd1b0becc9df1a877 agronomy-15-00287-v2.pdf 5acf45b59329e5e53e07928e5ad930a5cb41884e394ee0843ba65630e8882a13 agronomy-15-00287-v2.pdf Title: Sown Diversity Effects on the C and N Cycle and Interactions with Fertilization Subject: A better understanding of the role of plant composition and N cycle on agroecosystems is necessary, as these will be affected by future developments in agriculture intensification. To explore the effect of plant diversity on yield and carbon (C) and nitrogen (N) balances in forage mixtures, identifying potential co-benefits between functions. We analyzed results from a field experiment where plants of three forage species (a grass, a legume, and a non-legume forb) were cultivated in monocultures and mixtures. Three years after sward establishment, dry matter yield, together with 15N, 13C, and C and N content in plant and soil material were measured. In addition, we analyzed a second scenario to investigate the effect of fertigation with pig slurry (15N = +8.4‰) on the C and N balances of forage species. Results support the hypothesis that C and N allocation is affected by plant diversity. Plant composition affected N source (% N derived from air, % N derived from soil, and % N transferred in mixtures). In addition, sown diversity increased yield and modulated C and N balances. The 15N of samples was affected by both plant composition and fertigation. These results are consistent with previous work showing strong plant composition effects on N-balances, and the potential role that legumes play in enhancing nitrogen sources (derived from the atmosphere) into forage mixture systems. This study contributes to the prediction of suitable sown plant community composition and N management for the optimum agriculture with increased productivity and at the same time reduced environmental impact. Keywords: plant diversity; forage mixtures; carbon and nitrogen balance; stable isotope Author: Salvador Aljazairi, Angela Ribas, Rosa Llurba, Juan Pedro Ferrio, Jordi Voltas, Salvador Nogués and Maria Teresa Sebastiá Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Jan 24 08:14:51 2025 CET ModDate: Fri Jan 24 10:48:17 2025 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 26 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1671724 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EBLVEP+EURM10 Type 1 Builtin yes yes yes 10 0 FZSAMJ+VnURWPalladioL Type 1 Custom yes yes yes 15 0 UTHWAY+URWPalladioL-Roma Type 1 Custom yes yes yes 21 0 WLBIPG+URWPalladioL-Bold Type 1 Custom yes yes yes 27 0 OHZQUQ+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 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AEDNFG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 263 0 AEDOAD+PalatinoLinotype TrueType MacRoman yes yes no 266 0 AEEFLK+Calibri CID TrueType Identity-H yes yes yes 280 0 AEDNEE+PalatinoLinotype CID TrueType Identity-H yes yes yes 286 0 AEDNFG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 292 0 AEDNED+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 295 0 AEDNFE+PalatinoLinotype TrueType WinAnsi yes yes no 298 0 AEDOAD+PalatinoLinotype TrueType MacRoman yes yes no 301 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-05-29 02:42:22 CEST RepresentationInformation: agronomy-15-00287-v2.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-05-28 08:39:11 CEST Size: 1671724 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 825 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Site Description Destination: subsection.2.1 Item: Title: Irrigation and Fertilization Destination: subsection.2.2 Item: Title: Plant Yield Determination and Soil Sampling Destination: subsection.2.3 Item: Title: Isotopic Composition of C and N Destination: subsection.2.4 Children: Item: Title: Plant and Soil Sampling Destination: subsubsection.2.4.1 Item: Title: Measurement of Greenhouse Gas C and N Concentration and C Isotope Signatures Destination: subsubsection.2.4.2 Item: Title: Inputs Description Destination: subsubsection.2.4.3 Item: Title: Isotopic Signals Calculation Destination: subsubsection.2.4.4 Item: Title: Calculation of N Balances and N Isotope Balances Destination: subsubsection.2.4.5 Item: Title: Data Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Carbon Dynamics Destination: subsection.3.1 Children: Item: Title: 13C in Plant Material, Soil Organic Matter, and Respired CO2 (‰) Destination: subsubsection.3.1.1 Item: Title: C Content (mg/mg) Destination: subsubsection.3.1.2 Item: Title: Nitrogen Dynamics Destination: subsection.3.2 Children: Item: Title: 15N (‰) Destination: subsubsection.3.2.1 Item: Title: Estimation of the N Source Destination: subsubsection.3.2.2 Item: Title: N Content (mg/mg) Destination: subsubsection.3.2.3 Item: Title: Carbon/Nitrogen (C/N) Destination: subsection.3.3 Item: Title: Dry Matter Yield Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Children: Item: Title: Carbon Dynamics Destination: subsection.4.1 Item: Title: Nitrogen Dynamics Destination: subsection.4.2 Item: Title: Carbon and Nitrogen Balance (C/N) Destination: subsection.4.3 Item: Title: Dry Matter Yield (DMY) Destination: subsection.4.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Sown Diversity Effects on the C and N Cycle and Interactions with Fertilization Author: Salvador Aljazairi, Angela Ribas, Rosa Llurba, Juan Pedro Ferrio, Jordi Voltas, Salvador Nogués and Maria Teresa Sebastiá Subject: ぁ〠ぢづぴぴづひ〠ふのつづひびぴちのつどので〠はて〠ぴとづ〠ひはぬづ〠はて〠ばぬちのぴ〠っはねばはびどぴどはの〠ちのつ〠ぎ〠っべっぬづ〠はの〠ちでひはづっはびべびぴづねび〠どび〠のづっづびびちひべ〬〠ちび〠ぴとづびづ〠ぷどぬぬ〠ぢづ〠ちててづっぴづつ〠ぢべ〠てふぴふひづ〠つづぶづぬはばねづのぴび〠どの〠ちでひどっふぬぴふひづ〠どのぴづのびどてどっちぴどはの〮〠ごは〠づへばぬはひづ〠ぴとづ〠づててづっぴ〠はて〠ばぬちのぴ〠つどぶづひびどぴべ〠はの〠べどづぬつ〠ちのつ〠っちひぢはの〠〨ぃ〩〠ちのつ〠のどぴひはでづの〠〨ぎ〩〠ぢちぬちのっづび〠どの〠てはひちでづ〠ねどへぴふひづび〬〠どつづのぴどてべどので〠ばはぴづのぴどちぬ〠っは〭ぢづのづてどぴび〠ぢづぴぷづづの〠てふのっぴどはのび〮〠しづ〠ちのちぬべぺづつ〠ひづびふぬぴび〠てひはね〠ち〠てどづぬつ〠づへばづひどねづのぴ〠ぷとづひづ〠ばぬちのぴび〠はて〠ぴとひづづ〠てはひちでづ〠びばづっどづび〠〨ち〠でひちびび〬〠ち〠ぬづでふねづ〬〠ちのつ〠ち〠のはの〭ぬづでふねづ〠てはひぢ〩〠ぷづひづ〠っふぬぴどぶちぴづつ〠どの〠ねはのはっふぬぴふひづび〠ちのつ〠ねどへぴふひづび〮〠ごとひづづ〠べづちひび〠ちてぴづひ〠びぷちひつ〠づびぴちぢぬどびとねづのぴ〬〠つひべ〠ねちぴぴづひ〠べどづぬつ〬〠ぴはでづぴとづひ〠ぷどぴと〠〱〵ぎ〬〠〱〳ぃ〬〠ちのつ〠ぃ〠ちのつ〠ぎ〠っはのぴづのぴ〠どの〠ばぬちのぴ〠ちのつ〠びはどぬ〠ねちぴづひどちぬ〠ぷづひづ〠ねづちびふひづつ〮〠ぉの〠ちつつどぴどはの〬〠ぷづ〠ちのちぬべぺづつ〠ち〠びづっはのつ〠びっづのちひどは゠ぴは〠どのぶづびぴどでちぴづ〠ぴとづ〠づててづっぴ〠はて〠てづひぴどでちぴどはの〠ぷどぴと〠ばどで〠びぬふひひべ〠〨〱〵ぎ〠〽〠〫〸〮〴‰〩〠はの〠ぴとづ〠ぃ〠ちのつ〠ぎ〠ぢちぬちのっづび〠はて〠てはひちでづ〠びばづっどづび〮〠げづびふぬぴび〠びふばばはひぴ〠ぴとづ〠とべばはぴとづびどび〠ぴとちぴ〠ぃ〠ちのつ〠ぎ〠ちぬぬはっちぴどはの〠どび〠ちててづっぴづつ〠ぢべ〠ばぬちのぴ〠つどぶづひびどぴべ〮〠ぐぬちのぴ〠っはねばはびどぴどはの〠ちててづっぴづつ〠ぎ〠びはふひっづ〠〨〥〠ぎ〠つづひどぶづつ〠てひはね〠ちどひ〬〠〥〠ぎ〠つづひどぶづつ〠てひはね〠びはどぬ〬〠ちのつ〠〥〠ぎ〠ぴひちのびてづひひづつ〠どの〠ねどへぴふひづび〩〮〠ぉの〠ちつつどぴどはの〬〠びはぷの〠つどぶづひびどぴべ〠どのっひづちびづつ〠べどづぬつ〠ちのつ〠ねはつふぬちぴづつ〠ぃ〠ちのつ〠ぎ〠ぢちぬちのっづび〮〠ごとづ〠〱〵ぎ〠はて〠びちねばぬづび〠ぷちび〠ちててづっぴづつ〠ぢべ〠ぢはぴと〠ばぬちのぴ〠っはねばはびどぴどはの〠ちのつ〠てづひぴどでちぴどはの〮〠ごとづびづ〠ひづびふぬぴび〠ちひづ〠っはのびどびぴづのぴ〠ぷどぴと〠ばひづぶどはふび〠ぷはひに〠びとはぷどので〠びぴひはので〠ばぬちのぴ〠っはねばはびどぴどはの〠づててづっぴび〠はの〠ぎ〭ぢちぬちのっづび〬〠ちのつ〠ぴとづ〠ばはぴづのぴどちぬ〠ひはぬづ〠ぴとちぴ〠ぬづでふねづび〠ばぬちべ〠どの〠づのとちのっどので〠のどぴひはでづの〠びはふひっづび〠〨つづひどぶづつ〠てひはね〠ぴとづ〠ちぴねはびばとづひづ〩〠どのぴは〠てはひちでづ〠ねどへぴふひづ〠びべびぴづねび〮〠ごとどび〠びぴふつべ〠っはのぴひどぢふぴづび〠ぴは〠ぴとづ〠ばひづつどっぴどはの〠はて〠びふどぴちぢぬづ〠びはぷの〠ばぬちのぴ〠っはねねふのどぴべ〠っはねばはびどぴどはの〠ちのつ〠ぎ〠ねちのちでづねづのぴ〠てはひ〠ぴとづ〠はばぴどねふね〠ちでひどっふぬぴふひづ〠ぷどぴと〠どのっひづちびづつ〠ばひはつふっぴどぶどぴべ〠ちのつ〠ちぴ〠ぴとづ〠びちねづ〠ぴどねづ〠ひづつふっづつ〠づのぶどひはのねづのぴちぬ〠どねばちっぴ〮 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