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Isolation and characterization of extracellular vesicle subsets in donkey seminal plasma Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Material and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Animals, ejaculates and seminal plasma samples Destination: mk:H2_1 Item: Title: 2.2 Isolation of seminal extracellular vesicles subsets Destination: mk:H2_2 Item: Title: 2.3 Characterization of seminal extracellular vesicles subsets Destination: mk:H2_3 Children: Item: Title: 2.3.1 Total protein concentration Destination: mk:H3_4 Item: Title: 2.3.2 Particle and sEV concentration Destination: mk:H3_5 Item: Title: 2.3.3 Particle size distribution Destination: mk:H3_6 Item: Title: 2.3.4 Morphology and structure of sEVs Destination: mk:H3_7 Item: Title: 2.3.5 Immunophenotyping of sEVs Destination: mk:H3_8 Item: Title: 2.3.6 Purity of sEV samples Destination: mk:H3_9 Item: Title: 2.4 Statistical analysis Destination: mk:H2_10 Item: Title: 3 Results Destination: mk:H1_3 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Availability of data and material Destination: mk:H1_6 Item: Title: Funding Destination: mk:H1_7 Item: Title: Declaration of competing interest Destination: mk:HH1_3 Item: Title: Acknowledgments Destination: mk:HH1_4 Item: Title: Appendix A Supplementary data Destination: mk:H1_8 Item: Title: References Destination: mk:HH2_1 Info: Title: Isolation and characterization of extracellular vesicle subsets in donkey seminal plasma Author: Jaime Catalán Subject: Theriogenology, 244 (2025) 117501. doi:10.1016/j.theriogenology.2025.117501 Keywords: Donkey,Extracellular vesicles,Seminal plasma,Size-exclusion chromatography Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Jun 02 06:44:35 CEST 2025 ModDate: Mon Jun 02 06:46:10 CEST 2025 ID: 0xc9e04c909b3afea9b6e19a539e25cd8f, 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Theriogenology, 244 (2025) 117501. doi:10.1016/j.theriogenology.2025.117501 Donkey Extracellular vesicles Seminal plasma Size-exclusion chromatography Isolation and characterization of extracellular vesicle subsets in donkey seminal plasma Jaime Catalán Pablo Martínez-Díaz Ana Parra Sergi Bonet Marc Yeste Jordi Roca Isabel Barranco Jordi Miró VoR 2nd June 2025 Acrobat Distiller 8.1.0 (Windows) Donkey,Extracellular vesicles,Seminal plasma,Size-exclusion chromatography 2nd June 2025 sciencedirect.com elsevier.com true 2010-04-23 10.1016/j.theriogenology.2025.117501 noindex journal © 2025 The Authors. 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