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Rodi˜no-Janeiro Creator: LaTeX with hyperref package + hypdvips Producer: dvips + MiKTeX GPL Ghostscript 9.0 CreationDate: Wed Jan 15 06:01:49 2025 CET ModDate: Wed Jan 15 08:55:24 2025 CET Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.26 x 842.116 pts (A4) Page rot: 0 File size: 9330347 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AELREO+MuseoSans-700 Type 1C Custom yes yes yes 47 0 EHQLCR+MuseoSans-300 Type 1C Custom yes yes yes 50 0 EJKBVS+MuseoSans-300Italic Type 1C WinAnsi yes yes no 65 0 FZSUYT+MinionPro-Regular Type 1C Custom yes yes no 68 0 QGJPEA+MuseoSans-500 Type 1C Custom yes yes yes 75 0 SNBKIS+MTSY Type 1C Custom yes yes yes 78 0 IOLGNI+MTGU Type 1C Custom yes yes no 199 0 GLMASA+MinionPro-It Type 1C Custom yes yes no 233 0 CXGUNB+MuseoSans-500Italic Type 1C WinAnsi yes yes no 254 0 GBAUGV+RMTMI Type 1C WinAnsi yes yes no 259 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-09-16 03:53:27 CEST RepresentationInformation: 319142.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-09-15 13:55:09 CEST Size: 9330347 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 597 FreeObjects: 26 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Acute stress triggers sex-dependent rapid alterations in the human small intestine microbiota composition Destination: ariclecounter.1 Children: Item: Title: 1 Introduction Destination: section.0.1 Item: Title: 2 Materials and methods Destination: section.0.2 Children: Item: Title: 2.1 Participants Destination: subsection.0.2.1 Item: Title: 2.2 Jejunal biopsy Destination: subsection.0.2.2 Item: Title: 2.3 Cold pain stress Destination: subsection.0.2.3 Item: Title: 2.4 Systemic response to cold pain stress Destination: subsection.0.2.4 Children: Item: Title: 2.4.1 Hand pain perception Destination: subsubsection.0.2.4.1 Item: Title: 2.4.2 Autonomic response Destination: subsubsection.0.2.4.2 Item: Title: 2.4.3 Psychological response Destination: subsubsection.0.2.4.3 Item: Title: 2.4.4 Hormonal response Destination: subsubsection.0.2.4.4 Item: Title: 2.5 Experimental design Destination: subsection.0.2.5 Item: Title: 2.6 DNA extraction, library preparation and sequencing Destination: subsection.0.2.6 Item: Title: 2.7 Hormonal response Destination: subsection.0.2.7 Item: Title: 2.8 Microbiome analysis Destination: subsection.0.2.8 Item: Title: 2.9 Statistical analysis Destination: subsection.0.2.9 Item: Title: 3 Results Destination: section.0.3 Children: Item: Title: 3.1 Systemic response to CPS Destination: subsection.0.3.1 Children: Item: Title: 3.1.1 Autonomic response Destination: subsubsection.0.3.1.1 Item: Title: 3.1.2 Psychological response Destination: subsubsection.0.3.1.2 Item: Title: 3.1.3 Hand pain perception Destination: subsubsection.0.3.1.3 Item: Title: 3.1.4 Hormonal response Destination: subsubsection.0.3.1.4 Item: Title: 3.2 Effect of CPS on the jejunal microbiota Destination: subsection.0.3.2 Item: Title: 3.3 Effect of sex in the CPS-induced changes in the jejunal microbiota Destination: subsection.0.3.3 Item: Title: 3.4 Correlations between changes in the jejunal microbiota and molecular, hormonal and psychological responses to CPS Destination: subsection.0.3.4 Item: Title: 4 Discussion Destination: section.0.4 Item: Title: Data availability statement Destination: section*.27 Item: Title: Ethics statement Destination: section*.28 Item: Title: Author contributions Destination: section*.29 Item: Title: Funding Destination: section*.30 Item: Title: Conflict of interest Destination: section*.31 Item: Title: Publisher's note Destination: section*.32 Item: Title: Supplementary material Destination: section*.33 Item: Title: References Destination: section*.34 Info: Title: Acute stress triggers sex-dependent rapid alterations in the human small intestine microbiota composition Author: Bruno K. Rodi˜no-Janeiro Subject: Rodi˜no-Janeiro BK, Khannous-Lleiffe O, Pigrau M, Willis JR, Salvo-Romero E, Nieto A, Exp´osito E, Fortea M, Pardo-Camacho C, Albert-Bayo M, Gonz´alez-Castro AM, Guagnozzi D, Martı́nez C, Lobo B, Vicario M, Santos J, Gabald´on T and Alonso-Cotoner C (2025) Acute stress triggers sex-dependent rapid alterations in the human small intestine microbiota composition. Front. 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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Rodi˜no-Janeiro BK, Khannous-Lleiffe O, Pigrau M, Willis JR, Salvo-Romero E, Nieto A, Exp´osito E, Fortea M, Pardo-Camacho C, Albert-Bayo M, Gonz´alez-Castro AM, Guagnozzi D, Martı́nez C, Lobo B, Vicario M, Santos J, Gabald´on T and Alonso-Cotoner C (2025) Acute stress triggers sex-dependent rapid alterations in the human small intestine microbiota composition. Front. Microbiol. 15:1441126. doi: 10.3389/fmicb.2024.1441126 Bruno K. Rodi˜no-Janeiro Olfat Khannous-Lleiffe Marc Pigrau Jesse R. Willis Eloı́sa Salvo-Romero Adoraci´on Nieto Elba Exp´osito Marina Fortea Cristina Pardo-Camacho Merc´e Albert-Bayo Ana María Gonz´alez-Castro Danila Guagnozzi Cristina Martı́nez Beatriz Lobo Marı́a Vicario Javier Santos Toni Gabald´on Carmen Alonso-Cotone Acute stress triggers sex-dependent rapid alterations in the human small intestine microbiota composition stress functional dyspepsia disorders of gut-brain interaction irritable bowel syndrome small intestine microbiota uuid:bf76f872-e72d-4890-a1fb-33db81ffb084 uuid:ce0dd0cb-5991-4461-9452-d7b777d46a6a Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 48, 789, 235, 806 Annotation: Subtype: Link Rect: 454, 800, 513, 808 Annotation: Subtype: Link Rect: 471, 791, 481, 799 Annotation: Subtype: Link Rect: 481, 791, 508, 799 Annotation: Subtype: Link Rect: 508, 791, 526, 799 Annotation: Subtype: Link Rect: 451, 782, 546, 790 Annotation: Subtype: Link Rect: 48, 700, 124, 717 Annotation: Subtype: Link Rect: 57, 533, 182, 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Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Page: Label: 10 Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 450, 800, 546, 807 Annotation: Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Page: Label: 11 Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 450, 800, 546, 807 Annotation: Subtype: Link Rect: 465, 124, 502, 136 Destination: External Annotation: Subtype: Link Rect: 504, 124, 523, 136 Destination: External Annotation: Subtype: Link Rect: 358, 112, 395, 124 Destination: External Annotation: Subtype: Link Rect: 398, 112, 417, 124 Destination: External Annotation: Subtype: Link Rect: 420, 112, 439, 124 Destination: External Annotation: Subtype: Link Rect: 348, 100, 389, 112 Destination: External Annotation: Subtype: Link Rect: 392, 100, 411, 112 Destination: External Annotation: Subtype: Link Rect: 414, 100, 469, 112 Destination: External Annotation: Subtype: Link Rect: 471, 100, 491, 112 Destination: External Annotation: Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Page: Label: 12 Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 450, 800, 546, 807 Annotation: Subtype: Link Rect: 163, 740, 214, 752 Destination: External Annotation: Subtype: Link Rect: 217, 740, 237, 752 Destination: External Annotation: Subtype: Link Rect: 240, 740, 289, 752 Destination: External Annotation: Subtype: Link Rect: 48, 728, 67, 740 Destination: External Annotation: Subtype: Link Rect: 71, 728, 104, 740 Destination: External Annotation: Subtype: Link Rect: 108, 728, 127, 740 Destination: External Annotation: Subtype: Link Rect: 234, 692, 289, 704 Destination: External Annotation: Subtype: Link Rect: 48, 680, 67, 692 Destination: External Annotation: Subtype: Link Rect: 201, 656, 246, 668 Destination: External Annotation: Subtype: Link Rect: 251, 656, 270, 668 Destination: External Annotation: Subtype: Link Rect: 157, 644, 203, 656 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Annotation: Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Page: Label: 13 Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 450, 800, 546, 807 Annotation: Subtype: Link Rect: 71, 740, 128, 752 Destination: External Annotation: Subtype: Link Rect: 132, 740, 151, 752 Destination: External Annotation: Subtype: Link Rect: 155, 740, 205, 752 Destination: External Annotation: Subtype: Link Rect: 209, 740, 228, 752 Destination: External Annotation: Subtype: Link Rect: 232, 740, 263, 752 Destination: External Annotation: Subtype: Link Rect: 267, 740, 286, 752 Destination: External Annotation: Subtype: Link Rect: 158, 728, 193, 740 Destination: External Annotation: Subtype: Link Rect: 197, 728, 216, 740 Destination: External Annotation: Subtype: Link Rect: 146, 680, 229, 692 Destination: External Annotation: Subtype: Link Rect: 231, 680, 250, 692 Destination: External Annotation: Subtype: Link Rect: 252, 680, 67, 680 Destination: External Annotation: Subtype: Link Rect: 69, 668, 88, 680 Destination: External Annotation: Subtype: Link Rect: 209, 668, 289, 680 Destination: External Annotation: Subtype: Link Rect: 48, 656, 67, 668 Destination: External Annotation: Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Page: Label: 14 Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 450, 800, 546, 807 Annotation: Subtype: Link Rect: 547, 700, 302, 725 Annotation: Subtype: Link Rect: 104, 597, 176, 606 Annotation: Subtype: Link Rect: 60, 561, 142, 570 Annotation: Subtype: Link Rect: 116, 525, 212, 534 Annotation: Subtype: Link Rect: 60, 491, 153, 498 Annotation: Subtype: Link Rect: 60, 455, 143, 462 Annotation: Subtype: Link Rect: 181, 434, 242, 442 Annotation: Subtype: Link Rect: 60, 406, 174, 415 Annotation: Subtype: Link Rect: 127, 370, 216, 379 Annotation: Subtype: Link Rect: 151, 344, 210, 351 Annotation: Subtype: Link Rect: 60, 306, 132, 315 Annotation: Subtype: Link Rect: 60, 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Annotation: Subtype: Link Rect: 428, 517, 498, 524 Annotation: Subtype: Link Rect: 411, 495, 492, 504 Annotation: Subtype: Link Rect: 404, 468, 497, 476 Annotation: Subtype: Link Rect: 315, 433, 407, 440 Annotation: Subtype: Link Rect: 342, 412, 431, 420 Annotation: Subtype: Link Rect: 315, 376, 394, 384 Annotation: Subtype: Link Rect: 342, 348, 416, 356 Annotation: Subtype: Link Rect: 433, 320, 513, 329 Annotation: Subtype: Link Rect: 361, 292, 442, 301 Annotation: Subtype: Link Rect: 547, 257, 302, 273 Annotation: Subtype: Link Rect: 315, 220, 386, 229 Annotation: Subtype: Link Rect: 315, 184, 397, 193 Annotation: Subtype: Link Rect: 388, 164, 475, 173 Annotation: Subtype: Link Rect: 367, 128, 448, 137 Annotation: Subtype: Link Rect: 315, 93, 398, 101 Annotation: Subtype: Link Rect: 478, 74, 538, 81 Annotation: Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Page: Label: 16 Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 450, 800, 546, 807 Annotation: Subtype: Link Rect: 60, 726, 147, 735 Annotation: Subtype: Link Rect: 184, 698, 281, 707 Annotation: Subtype: Link Rect: 87, 670, 160, 679 Annotation: Subtype: Link Rect: 200, 643, 281, 651 Annotation: Subtype: Link Rect: 104, 607, 185, 616 Annotation: Subtype: Link Rect: 60, 571, 142, 579 Annotation: Subtype: Link Rect: 94, 535, 175, 544 Annotation: Subtype: Link Rect: 315, 742, 387, 751 Annotation: Subtype: Link Rect: 315, 716, 392, 723 Annotation: Subtype: Link Rect: 360, 695, 424, 703 Annotation: Subtype: Link Rect: 547, 651, 302, 668 Annotation: Subtype: Link Rect: 334, 624, 416, 631 Annotation: Subtype: Link Rect: 315, 588, 394, 596 Annotation: Subtype: Link Rect: 350, 552, 435, 559 Annotation: Subtype: Link Rect: 315, 515, 406, 524 Annotation: Subtype: Link Rect: 48, 33, 130, 41 Annotation: Subtype: Link Rect: 498, 33, 546, 41 Checksum: dd6b8b3c Type: CRC32 Checksum: fbe66346c0d75a48f6025c0036396f96 Type: MD5 Checksum: 8e2eef584939c465b64931c2838eedb43d3ebe37 Type: SHA-1 Checksum: 0135ea36a9781fc97484c6d7a57b9032fe87c340cca91d3cbc22c5f39242f765 Type: SHA-256