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Destination: titlink1 Children: Item: Title: 1. Introduction Destination: flink1 Item: Title: 2. Methods Destination: flink2 Children: Item: Title: 2.1. Study design Destination: slink1 Item: Title: 2.2. Sample collection Destination: slink2 Item: Title: 2.3. Laboratory clinics Destination: slink3 Item: Title: 2.4. ELISA Destination: slink4 Children: Item: Title: 2.4.1. QUANTA Lite ACA HRP IgG III ELISA (Werfen, #708625) Destination: thirlink1 Item: Title: 2.4.2. QUANTA Lite β2GPI IgG ELISA (Werfen, #708665) Destination: thirlink2 Item: Title: 2.4.3. aCL IgG/IgM (Orgentec Diagnostika GmbH, #ORG515) Destination: thirlink3 Item: Title: 2.4.4. aβ2GPI IgG/IgM (Orgentec Diagnostika GmbH, #ORG521) Destination: thirlink4 Item: Title: 2.4.5. AESKULISA Cardiolipin-GM (AESKU Diagnostics, #3204) Destination: thirlink5 Item: Title: 2.4.6. AESKULISA β2-Glyco-GM (AESKU Diagnostics, #3206) Destination: thirlink6 Item: Title: 2.5. Coefficients of variation Destination: slink5 Item: Title: 2.6. CLIAs Destination: slink6 Item: Title: 2.7. Outlier detection Destination: slink7 Item: Title: 2.8. Statistical analysis Destination: slink8 Item: Title: 3. Results Destination: flink3 Children: Item: Title: 3.1. aCL and aβ2GPI 99th percentile in different commercial platforms Destination: slink9 Item: Title: 3.2. Standardized calibrators differ between platforms Destination: slink10 Item: Title: 3.3. Consistency of aPL positivity between platforms in a cohort of APS patients Destination: slink11 Item: Title: 4. Discussion Destination: flink4 Item: Title: 5. 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854, 927 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: GPHHEL+AdvOT21de4310 FontSubset: true FirstChar: 35 LastChar: 252 FontDescriptor: FontName: GPHHEL+AdvOT21de4310 Flags: Nonsymbolic FontBBox: -28, -182, 1011, 775 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: GPHHEM+AdvOTe3b1fbf3.B FontSubset: true FirstChar: 45 LastChar: 45 FontDescriptor: FontName: GPHHEM+AdvOTe3b1fbf3.B Flags: Nonsymbolic FontBBox: -62, -208, 979, 1000 FontFile3: true Encoding: WinAnsiEncoding XMP: application/pdf doi:10.1016/j.rpth.2025.102967 The absence of standardization in antiphospholipid antibody testing may favor the use of 99th percentile cutoffs in antiphospholipid syndrome classification Ariadna Anunciación-Llunell Joana Marques-Soares Monika Ockova Natalia Pozuelo Enrique Esteve-Valverde Catalina Andrada Jaume Alijotas-Reig Francesc A. Miró-Mur anti-β2 glycoprotein I anticardiolipin antiphospholipid syndrome chemiluminescent assay ELISA reference values Research and Practice in Thrombosis and Haemostasis, 9 (2025) 102967. doi:10.1016/j.rpth.2025.102967 The Authors journal Research and Practice in Thrombosis and Haemostasis © 2025 The Authors. 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