1aefae76965e29b3d686158ceb80cfd7 319544.pdf 7136a9120a1c5d607714a71d5318450f231ebedb 319544.pdf dc5f704ddee369bcbad7231317a40584c88b32d5d4b8a7a5b850930c3320cf3f 319544.pdf Title: Diagnostic and Prognostic Value of hsa_piR_022710, hsa_piR_019822, and hsa_piR_020840 in Early-Stage Non-Small-Cell Lung Cancer: Implications for Recurrenceand Survival in Squamous Cell Carcinoma Patients Subject: Despite significant advancements in early detection and treatment, non-small-cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality. Specifically, in early-stage cases, recurrence after surgery continues to be the principal cause of death for these patients. The urgent need for novel diagnostic and prognostic biomarkers has directed attention towards PIWI-interacting RNAs (piRNAs), a group of small RNAs that regulate genomic stability and epigenetics. Some piRNAs, including hsa_piR_022710, hsa_piR_019822, and hsa_piR_020840, have been described as deregulated in various cancers. This study investigated the expression of these three piRNAs by RT-qPCR in 277 NSCLC patients and developed survival and CART classification models to predict recurrence risk, overall survival (OS), and disease-free survival (DFS). hsa_piR_019822 and hsa_piR_020840 were able to discriminate between tumor and normal tissue, as well as between adenocarcinoma and squamous cell carcinoma (LUSC) patients. Elevated expression of hsa_piR_019822 and hsa_piR_022710 was correlated with an increased risk of recurrence and poorer DFS and OS in LUSC patients. Patients with high hsa_piR_022710 expression more greatly benefited from adjuvant treatment. In summary, higher piRNA levels were associated with an increased risk of recurrence and poorer survival outcomes, especially in LUSC patients, where they may help guide personalized treatment strategies. Keywords: NSCLC; squamous cell carcinoma; piRNA; recurrence; prognosis; biomarkers Author: Yangyi He, Antonio Altuna-Coy, Melissa Acosta-Plasencia, Laureano Molins, David Sánchez-Lorente, Daniel Martinez, Tania Díaz, Risha Na, Ramón M. Marrades and Alfons Navarro Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Mar 21 15:40:00 2025 CET ModDate: Fri Mar 21 15:46:11 2025 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 18 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6211975 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QKZKVH+VnURWPalladioL Type 1 Custom yes yes yes 10 0 JSKBJU+URWPalladioL-Roma Type 1 Custom yes yes yes 16 0 KQAZBL+URWPalladioL-Bold Type 1 Custom yes yes yes 22 0 CXSROX+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 HBHLLH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 75 0 HBHLLJ+PalatinoLinotype TrueType WinAnsi yes yes no 78 0 HBHLLL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 81 0 HBHMBK+PalatinoLinotype TrueType MacRoman yes yes no 84 0 QLADRS+CMSY10 Type 1 Builtin yes yes yes 127 0 HBHLLH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 145 0 HBHLLI+PalatinoLinotype CID TrueType Identity-H yes yes yes 148 0 HBHLLJ+PalatinoLinotype TrueType WinAnsi yes yes no 154 0 HBHLLL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 157 0 HBHMBK+PalatinoLinotype TrueType MacRoman yes yes no 160 0 JCDKLJ+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 174 0 JCDKLK+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 180 0 JCDKLJ+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 209 0 JCDKLK+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 215 0 JCDPGO+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 218 0 HBHLLH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 268 0 HBHLLI+PalatinoLinotype CID TrueType Identity-H yes yes yes 271 0 HBHLLJ+PalatinoLinotype TrueType WinAnsi yes yes no 277 0 HBHLLL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 280 0 HBHMBK+PalatinoLinotype TrueType MacRoman yes yes no 283 0 HBHLLH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 358 0 HBHLLI+PalatinoLinotype CID TrueType Identity-H yes yes yes 361 0 HBHLLJ+PalatinoLinotype TrueType WinAnsi yes yes no 367 0 HBHLLL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 370 0 HBHMBK+PalatinoLinotype TrueType MacRoman yes yes no 373 0 CKBPVA+EURM10 Type 1 Builtin yes yes yes 435 0 MPUDFL+PazoMath Type 1 Builtin yes yes yes 440 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-10-04 04:07:01 CEST RepresentationInformation: 319544.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-10-03 11:28:39 CEST Size: 6211975 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 619 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Clinical and Pathological Characteristics of Patients Destination: subsection.2.1 Item: Title: Patient Prognostic Predictors Destination: subsection.2.2 Item: Title: PiRNAs Expression in Patient Sample Destination: subsection.2.3 Item: Title: Elevated piRNA Expression Levels Correlate with Higher Recurrence Risk in LUSC Patients Destination: subsection.2.4 Item: Title: Elevated piRNA Expression Levels Correlate with Poor DFS in LUSC Patients During Short-Term Follow-Up Destination: subsection.2.5 Item: Title: Elevated piRNA Expression Levels Correlate with Poor OS in LUSC Patients During Short-Term Follow-Up Destination: subsection.2.6 Item: Title: Prognostic Impact of piRNA Expression Levels on Recurrence, Survival, and the Role of Adjuvant Treatment Destination: subsection.2.7 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Patient Sample Destination: subsection.4.1 Item: Title: RNA Extraction and piRNA Quantification Destination: subsection.4.2 Item: Title: Statistical Analyses Destination: subsection.4.3 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Diagnostic and Prognostic Value of hsa_piR_022710, hsa_piR_019822, and hsa_piR_020840 in Early-Stage Non-Small-Cell Lung Cancer: Implications for Recurrenceand Survival in Squamous Cell Carcinoma Patients Author: Yangyi He, Antonio Altuna-Coy, Melissa Acosta-Plasencia, Laureano Molins, David Sánchez-Lorente, Daniel Martinez, Tania Díaz, Risha Na, Ramón M. Marrades and Alfons Navarro Subject: Despite significant advancements in early detection and treatment, non-small-cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality. Specifically, in early-stage cases, recurrence after surgery continues to be the principal cause of death for these patients. The urgent need for novel diagnostic and prognostic biomarkers has directed attention towards PIWI-interacting RNAs (piRNAs), a group of small RNAs that regulate genomic stability and epigenetics. Some piRNAs, including hsa_piR_022710, hsa_piR_019822, and hsa_piR_020840, have been described as deregulated in various cancers. This study investigated the expression of these three piRNAs by RT-qPCR in 277 NSCLC patients and developed survival and CART classification models to predict recurrence risk, overall survival (OS), and disease-free survival (DFS). hsa_piR_019822 and hsa_piR_020840 were able to discriminate between tumor and normal tissue, as well as between adenocarcinoma and squamous cell carcinoma (LUSC) patients. Elevated expression of hsa_piR_019822 and hsa_piR_022710 was correlated with an increased risk of recurrence and poorer DFS and OS in LUSC patients. Patients with high hsa_piR_022710 expression more greatly benefited from adjuvant treatment. In summary, higher piRNA levels were associated with an increased risk of recurrence and poorer survival outcomes, especially in LUSC patients, where they may help guide personalized treatment strategies. 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