4cc6b8c982bdf0fb45b47d568fb390a4 319545.pdf 40bc1f7d9d4bff64f055c07d4d6e94eb764f6f24 319545.pdf 394faaccb011890378a7c7410749b5f4429dda85d12ae181150bc332ee0f258d 319545.pdf Title: Erythrocyte Fatty Acid Profile, Mediterranean Diet and Asthma Severity in Childhood Allergic Asthma: Preliminary Findings from a Cohort Study in Spain Subject: Background: Allergic asthma incidence is increasing, probably due to the influence of the Western diet. Adherence to the Mediterranean diet (MedDi) and omega-3 fatty acids composition (n-3 PUFAs) may be linked to a lower prevalence and lower severity of childhood asthma; however, the association is inconclusive. This study aims to examine the relationship between adherence to the MedDi, asthma severity, and erythrocyte fatty acid profile in Spanish children with atopic asthma. Methods: This study was based on an ongoing single-center, prospective, cross-sectional cohort study involving 95 consecutively enrolled children from an outpatient tertiary referral center. Of these, 55 had atopic asthma and 40 were age-matched healthy controls. Blood samples were collected to analyze the erythrocyte fatty acid content. Participants’ demographic and clinical characteristics were recorded using validated self-reported outcome measures. Asthma severity and pulmonary function were assessed. Results: Asthmatics presented poorer adherence to the MedDi (p = 0.034) and lower n-3 PUFA levels (p = 0.019). Asthmatics with poor adherence to the MedDi were more likely to be overweight (p = 0.001) and to have moderate-severe asthma (p = 0.049); and lower n-3 PUFAs levels (p = 0.033). Children with mild asthma had higher n-3 PUFAs levels than those with moderate or severe asthma (p = 0.036). Conclusions: These findings highlight that adherence to the MedDi and a high erythrocyte fatty acid profile seem to have a protective effect in childhood asthma. Future well-controlled interventions should focus on the effects of MedDi patterns and n-3 PUFA intake on the primary prevention of childhood asthma. Keywords: mediterranean diet; erythrocyte fatty acids; omega-3 index; PUFAs; inflammation; asthma Author: Roser Ayats-Vidal, Isabela-Adelina Albiciuc, Carlota Bruch-Molist, Anna Cuartero-Gorjón, Begoña Cordobilla, Marina Pedrosa-Domínguez, Marta Susanna-Calero, Miguel García-González, Laura Valdesoiro-Navarrete, Helena Larramona-Carrera, Oscar Asensio-de la Cruz, Jesus Castro-Marrero and Joan Carles Domingo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Thu Mar 27 09:38:15 2025 CET ModDate: Thu Mar 27 09:53:55 2025 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1361809 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AIRHKL+VnURWPalladioL Type 1 Custom yes yes yes 10 0 DVVORB+URWPalladioL-Roma Type 1 Custom yes yes yes 16 0 XWTBVL+URWPalladioL-Bold Type 1 Custom yes yes yes 22 0 TJLSZW+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 LFLYLN+CMSY10 Type 1 Builtin yes yes yes 56 0 ALMJEC+EURM10 Type 1 Builtin yes yes yes 64 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes yes 72 0 MPHOBI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 87 0 MPHOBJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 90 0 MPHOCK+PalatinoLinotype TrueType WinAnsi yes yes no 96 0 MPHOCM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 99 0 MPHOOK+PalatinoLinotype TrueType MacRoman yes yes no 102 0 MPIDED+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 136 0 MPHOBI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 139 0 MPHOBJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 142 0 MPHOCK+PalatinoLinotype TrueType WinAnsi yes yes no 148 0 MPHOCM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 151 0 MPHOOK+PalatinoLinotype TrueType MacRoman yes yes no 154 0 MPHOBI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 188 0 MPHOBJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 191 0 MPHOCK+PalatinoLinotype TrueType WinAnsi yes yes no 197 0 MPHOCM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 200 0 MPHOOK+PalatinoLinotype TrueType MacRoman yes yes no 203 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-11-19 04:10:23 CET RepresentationInformation: 319545.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-11-18 08:35:25 CET Size: 1361809 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 366 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Methods Destination: section.2 Children: Item: Title: Participants and Study Design Destination: subsection.2.1 Item: Title: Ethics Statements Destination: subsection.2.2 Item: Title: Study Intervention and Data Collection Destination: subsection.2.3 Item: Title: Blood Collection and Erythrocyte Fatty Acid Analysis Destination: subsection.2.4 Item: Title: Statistical Analyses Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Population Characteristics Destination: subsection.3.1 Item: Title: Comparison Between Asthmatic Children with Good and Poor Adherence to the Mediterranean Diet Destination: subsection.3.2 Item: Title: Comparison Between Children with Mild Asthma Versus Children with Moderate or Severe Asthma Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Erythrocyte Fatty Acid Profile, Mediterranean Diet and Asthma Severity in Childhood Allergic Asthma: Preliminary Findings from a Cohort Study in Spain Author: Roser Ayats-Vidal, Isabela-Adelina Albiciuc, Carlota Bruch-Molist, Anna Cuartero-Gorjón, Begoña Cordobilla, Marina Pedrosa-Domínguez, Marta Susanna-Calero, Miguel García-González, Laura Valdesoiro-Navarrete, Helena Larramona-Carrera, Oscar Asensio-de la Cruz, Jesus Castro-Marrero and Joan Carles Domingo Subject: Background: Allergic asthma incidence is increasing, probably due to the influence of the Western diet. Adherence to the Mediterranean diet (MedDi) and omega-3 fatty acids composition (n-3 PUFAs) may be linked to a lower prevalence and lower severity of childhood asthma; however, the association is inconclusive. This study aims to examine the relationship between adherence to the MedDi, asthma severity, and erythrocyte fatty acid profile in Spanish children with atopic asthma. Methods: This study was based on an ongoing single-center, prospective, cross-sectional cohort study involving 95 consecutively enrolled children from an outpatient tertiary referral center. Of these, 55 had atopic asthma and 40 were age-matched healthy controls. Blood samples were collected to analyze the erythrocyte fatty acid content. Participants’ demographic and clinical characteristics were recorded using validated self-reported outcome measures. Asthma severity and pulmonary function were assessed. Results: Asthmatics presented poorer adherence to the MedDi (p = 0.034) and lower n-3 PUFA levels (p = 0.019). Asthmatics with poor adherence to the MedDi were more likely to be overweight (p = 0.001) and to have moderate-severe asthma (p = 0.049); and lower n-3 PUFAs levels (p = 0.033). Children with mild asthma had higher n-3 PUFAs levels than those with moderate or severe asthma (p = 0.036). Conclusions: These findings highlight that adherence to the MedDi and a high erythrocyte fatty acid profile seem to have a protective effect in childhood asthma. Future well-controlled interventions should focus on the effects of MedDi patterns and n-3 PUFA intake on the primary prevention of childhood asthma. 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