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Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Animals and general procedure Destination: mk:H2_1 Item: Title: 2.2 Immobilization stress Destination: mk:H2_2 Children: Item: Title: 2.2.1 Experiment 1: Time-course of c-fos response following 8-min IMO Destination: mk:H3_3 Item: Title: 2.2.2 Experiment 2: Time-course of c-fos response following 15-min IMO Destination: mk:H3_4 Item: Title: 2.2.3 Experiment 3: Comparison of FOS ​+ ​neurons between two time-matched IMO paradigms: sustained exposure versus recover ... Destination: mk:H3_5 Item: Title: 2.3 Perfusion and histological processing Destination: mk:H2_6 Children: Item: Title: 2.3.1 Double fluorescent in situ hybridization (dFISH) probes Destination: mk:H3_7 Item: Title: 2.3.2 dFISH procedure Destination: mk:H3_8 Item: Title: 2.3.3 Immunofluorescence (IF) Destination: mk:H3_9 Item: Title: 2.4 Image capture and analysis Destination: mk:H2_10 Item: Title: 2.5 Statistics Destination: mk:H2_11 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 Time-course of c-fos response following 8-min IMO Destination: mk:H2_12 Item: Title: 3.2 Time-course of c-fos response following 15 ​min IMO Destination: mk:H2_13 Item: Title: 3.3 Comparison of FOS ​+ ​neurons between two time-matched IMO paradigms: sustained exposure versus recovery phase Destination: mk:H2_14 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: Funding Destination: mk:H1_5 Item: Title: CRediT authorship contribution statement Destination: mk:H1_6 Item: Title: 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Neurobiology of Stress, 36 (2025) 100729. doi:10.1016/j.ynstr.2025.100729 c-fos expression Immobilization stress Stress duration Neuronal activation Prelimbic cortex Dynamics of stress-induced c-fos expression in the rat prelimbic cortex: lessons from intronic and mature RNA and protein analyses Patricia Molina Xavier Belda Sandra Beriain Sara Serrano Gentzane Compte Raül Andero Antonio Armario VoR 31st May 2025 Acrobat Distiller 8.1.0 (Windows) c-fos expression,Immobilization stress,Stress duration,Neuronal activation,Prelimbic cortex 31st May 2025 sciencedirect.com elsevier.com true 2010-04-23 10.1016/j.ynstr.2025.100729 noindex journal © 2025 The Authors. 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