6e72f592fe2270ec44f12d837a8b3cf9 biologics-05-00019.pdf d507edbaf213d73c3d9e25f1333debe5c1074154 biologics-05-00019.pdf d2a6a4653436b725b65fd07d36751123de1b48e1a747eff727f0e7348bc9a4b5 biologics-05-00019.pdf Title: Antiproliferative Potential of Eugenia uniflora L. Leaf Essential Oil in Normal and Tumoral Human Colon Cells Subject: Background/Objectives: Natural products are important in healthcare due to their accessibility and linkage to a healthy lifestyle. However, their effectiveness is uncertain due to insufficient scientific data. Cancer patients are frequent users of natural products to relieve symptoms or for chemoprevention. Eugenia uniflora leaf essential oil (EO), traditionally used for digestive disorders, emerges as a potential antineoplastic agent. We investigated the cytotoxic and antiproliferative effects of E. uniflora EO in human normal CCD 841 CoN and tumoral Caco-2 colonic cell lines. Methods: CCD 841 CoN and Caco-2 cells were exposed to different concentrations of E. uniflora EO, and the cytotoxicity was determined by MTT and Trypan Blue assays. Cell proliferation kinetics were analyzed at a low EO concentration, and the induction of DNA damage and oxidative stress was assessed by Comet and Cellular ROS assays. Results: Both cell lines exhibited cytotoxicity produced by the EO and decreased cell viability of the exposed cells and their progeny. CCD 841 CoN proliferation was impaired by low EO concentration, while the proliferation kinetics of the Caco-2 cells was modified. EO treatment induced variable DNA damage and oxidative stress depending on the cell line. Conclusions: Our results suggest that E. uniflora EO may prevent the proliferation of normal cells, inducing loss of viability. The EO produced cytotoxic and antiproliferative effects in tumoral cells by inducing DNA damage and increased oxidative stress. These effects support the consideration of E. uniflora EO (or its bioactive compounds) as a potential agent for the chemoprevention and treatment of colorectal cancer. Keywords: Eugenia uniflora; essential oil; colorectal cancer; CCD 841 CoN cells; Caco-2 cells; chemoprevention; chemotherapy; antiproliferative effect; cytotoxicity Author: Ana G. Sánchez, Macarena Menoni, Pamela Lombardo, Eduardo Dellacassa, María Angélica Severi, Gabriela Ferragut, Beatriz Vignale, Juan Cedano, María José Zuluaga and Deborah J. Keszenman Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Thu Jul 3 13:12:01 2025 CEST ModDate: Thu Jul 3 13:58:51 2025 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2318752 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WVNDGF+URWPalladioL-Roma Type 1 Custom yes yes yes 10 0 ZDPVEK+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 JUCIOM+URWPalladioL-Ital Type 1 Custom yes yes yes 21 0 FEVZFK+URWPalladioL-BoldItal Type 1 Custom yes yes yes 26 0 KJMJDX+CMSY10 Type 1 Builtin yes yes yes 61 0 OYEURL+EURM10 Type 1 Builtin yes yes yes 69 0 GIGFZE+CMR10 Type 1 Builtin yes yes yes 77 0 DDLNAC+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 94 0 DDLNAD+PalatinoLinotype-Roman CID TrueType 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Identity-H yes yes yes 335 0 DDLNAE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 341 0 VOGQER+VnURWPalladioL Type 1 Custom yes yes yes 368 0 ITYRUV+VnURWPalladioL-Italic Type 1 Custom yes yes yes 374 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-09-17 03:09:45 CEST RepresentationInformation: biologics-05-00019.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-09-16 13:25:31 CEST Size: 2318752 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 644 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Herbal Material Destination: subsection.2.1 Item: Title: Essential Oil Extraction and Solutions Destination: subsection.2.2 Item: Title: Essential Oil Analysis: Gas Chromatography Coupled with Mass Spectrometry (GC-MS) Destination: subsection.2.3 Item: Title: Cell Culture Destination: subsection.2.4 Item: Title: MTT Assay Destination: subsection.2.5 Item: Title: Trypan Blue Viability Assay Destination: subsection.2.6 Item: Title: Assessment of Cell Proliferation Kinetics Destination: subsection.2.7 Item: Title: DNA Damage Assay Destination: subsection.2.8 Item: Title: Reactive Oxygen Species Detection Assay Destination: subsection.2.9 Item: Title: Statistical Analysis Destination: subsection.2.10 Item: Title: Results Destination: section.3 Children: Item: Title: Chemical Characterization of E. uniflora EO Destination: subsection.3.1 Item: Title: Microscopic Morphology of Colonic Cell Lines Destination: subsection.3.2 Item: Title: Evaluation of the Cytotoxic Effect of E. uniflora EO on Cell Lines Destination: subsection.3.3 Item: Title: Cell Proliferation Kinetics of CCD 841 CoN and Caco-2 Cell Lines Destination: subsection.3.4 Item: Title: DNA Damage Produced by the E. uniflora EO Destination: subsection.3.5 Item: Title: Oxidative Stress Produced by E. uniflora EO Destination: subsection.3.6 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Antiproliferative Potential of Eugenia uniflora L. Leaf Essential Oil in Normal and Tumoral Human Colon Cells Author: Ana G. Sánchez, Macarena Menoni, Pamela Lombardo, Eduardo Dellacassa, María Angélica Severi, Gabriela Ferragut, Beatriz Vignale, Juan Cedano, María José Zuluaga and Deborah J. Keszenman Subject: Background/Objectives: Natural products are important in healthcare due to their accessibility and linkage to a healthy lifestyle. However, their effectiveness is uncertain due to insufficient scientific data. Cancer patients are frequent users of natural products to relieve symptoms or for chemoprevention. Eugenia uniflora leaf essential oil (EO), traditionally used for digestive disorders, emerges as a potential antineoplastic agent. We investigated the cytotoxic and antiproliferative effects of E. uniflora EO in human normal CCD 841 CoN and tumoral Caco-2 colonic cell lines. Methods: CCD 841 CoN and Caco-2 cells were exposed to different concentrations of E. uniflora EO, and the cytotoxicity was determined by MTT and Trypan Blue assays. Cell proliferation kinetics were analyzed at a low EO concentration, and the induction of DNA damage and oxidative stress was assessed by Comet and Cellular ROS assays. Results: Both cell lines exhibited cytotoxicity produced by the EO and decreased cell viability of the exposed cells and their progeny. CCD 841 CoN proliferation was impaired by low EO concentration, while the proliferation kinetics of the Caco-2 cells was modified. EO treatment induced variable DNA damage and oxidative stress depending on the cell line. Conclusions: Our results suggest that E. uniflora EO may prevent the proliferation of normal cells, inducing loss of viability. The EO produced cytotoxic and antiproliferative effects in tumoral cells by inducing DNA damage and increased oxidative stress. These effects support the consideration of E. uniflora EO (or its bioactive compounds) as a potential agent for the chemoprevention and treatment of colorectal cancer. 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335, 360, 346 Annotation: Subtype: Link Rect: 512, 322, 550, 333 Annotation: Subtype: Link Rect: 131, 297, 168, 307 Annotation: Subtype: Link Rect: 175, 297, 211, 307 Annotation: Subtype: Link Rect: 254, 259, 291, 269 Annotation: Subtype: Link Rect: 386, 220, 423, 231 Annotation: Subtype: Link Rect: 429, 220, 465, 231 Annotation: Subtype: Link Rect: 59, 182, 96, 193 Checksum: c6377dec Type: CRC32 Checksum: 6e72f592fe2270ec44f12d837a8b3cf9 Type: MD5 Checksum: d507edbaf213d73c3d9e25f1333debe5c1074154 Type: SHA-1 Checksum: d2a6a4653436b725b65fd07d36751123de1b48e1a747eff727f0e7348bc9a4b5 Type: SHA-256