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Mateu Creator: Adobe InDesign 19.5 (Windows) Producer: Adobe PDF Library 17.0 CreationDate: Fri Aug 22 11:56:40 2025 CEST ModDate: Fri Aug 22 11:57:07 2025 CEST Custom Metadata: no Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3308808 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NBPXTX+MuseoSans-500 Type 1C Custom yes yes yes 868 0 AFBXTX+MuseoSans-300 Type 1C Custom yes yes yes 875 0 LGOLRB+MuseoSans-700 Type 1C Custom yes yes yes 876 0 ZOKMFZ+MuseoSans-300Italic Type 1C WinAnsi yes yes yes 884 0 UEXINJ+MinionPro-Regular Type 1C Custom yes yes yes 152 0 MVVHFZ+MinionPro-It Type 1C Custom yes yes yes 147 0 RVEGXP+STIX-Regular Type 1C WinAnsi yes yes yes 143 0 CWRPVT+STIXGeneral-Regular Type 1C Custom yes yes yes 163 0 MTPXFZ+STIXGeneral-Italic Type 1C Custom yes yes yes 158 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-10-02 03:52:55 CEST RepresentationInformation: FRO_a2025m8d22.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-10-01 14:02:55 CEST Size: 3308808 Format: PDF Version: 1.6 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ErrorMessage: Annotation object is not a dictionary ID: PDF-HUL-114 Offset: 78295 MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 911 FreeObjects: 616 IncrementalUpdates: 1 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-US Outlines: Item: Title: Efficacy of an intranasally administered live attenuated PRRSV-2 vaccine against challenge with a highly virulent PRRSV-1 strain Children: Item: Title: 1 Introduction Item: Title: 2 Materials and methods Children: Item: Title: 2.1 Design of the study Item: Title: 2.2 Sampling Item: Title: 2.3 Clinical scores Item: Title: 2.4 Weight gains Item: Title: 2.5 Macroscopic and microscopic lung lesion scoring Item: Title: 2.6 Viremia, nasal shedding and viral load in lungs Item: Title: 2.7 Antibody detection Item: Title: 2.8 IFN- γ ELISPOT Item: Title: 2.9 Statistical analysis Item: Title: 3 Results Children: Item: Title: 3.1 Clinical scoring Item: Title: 3.2 Weight gains Item: Title: 3.3 Lung lesions Item: Title: 3.4 Viremia, nasal shedding and viral load in lungs Item: Title: 3.5 Antibodies Item: Title: 3.6 IFN- γ ELISPOT Item: Title: 4 Discussion Item: Title: References Info: Title: Efficacy of an intranasally administered live attenuated PRRSV-2 vaccine against challenge with a highly virulent PRRSV-1 strain Author: E. 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2025-08-22T15:27:07+05:30 2025-08-22T15:27:07+05:30 Adobe InDesign 19.5 (Windows) application/pdf Efficacy of an intranasally administered live attenuated PRRSV-2 vaccine against challenge with a highly virulent PRRSV-1 strain E. Mateu M. Cortey M. S. Serena I. Domingo-Carreño M. Alberch L. Aguirre I. Diaz M. Martín J. M. Sanchez-Carvajal I. M. Rodriguez-Gomez J. H. Lara-Puente C. Artigas-Cabre D. Sarfati-Mizrahi B. Lozano-Dubernard Mateu E, Cortey M, Serena MS, ​Domingo-Carreño I, Alberch M, Aguirre L, Diaz I, Martín M, ​Sanchez-Carvajal JM, ​Rodriguez-Gomez IM, Lara-Puente JH, Artigas-Cabre C, Sarfati-Mizrahi D and Lozano-Dubernard B (2025) Efficacy of an intranasally administered live attenuated PRRSV-2 vaccine against challenge with a highly virulent PRRSV-1 strain. Front. Vet. Sci. 12:1619052. doi: 10.3389/fvets.2025.1619052 porcine reproductive and respiratory syndrome virus intranasal vaccine immunity virulence clinical signs lung pathology Copyright © 2025 Mateu, Cortey, Serena, Domingo-Carreño, Alberch, Aguirre, Diaz, Martín, Sanchez-Carvajal, Rodriguez-Gomez, Lara-Puente, Artigas-Cabre, Sarfati-Mizrahi and Lozano-Dubernard. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 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