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Title: Introduction Destination: flink1 Item: Title: Results Destination: flink2 Children: Item: Title: Exploration data analysis of methylation patterns Destination: slink3 Item: Title: Apolipoprotein E and mitochondrial DNA methylation levels Destination: slink4 Item: Title: Mitochondrial DNA methylation levels analysis in negative Aβ-PET Scan subjects Destination: slink5 Item: Title: Differential methylation analysis Destination: slink6 Item: Title: Multiclass classification model Destination: slink7 Item: Title: Model comparison Destination: slink8 Item: Title: Discussion Destination: flink9 Children: Item: Title: Limitations of the study Destination: slink10 Item: Title: Resource availability Destination: flink11 Children: Item: Title: Lead contact Destination: slink12 Item: Title: Materials availability Destination: slink13 Item: Title: Data and code availability Destination: slink14 Item: Title: Acknowledgments Destination: flink15 Item: Title: Author contributions Destination: flink16 Item: Title: Declaration of interests Destination: flink17 Item: Title: Supplemental information Destination: falink18 Item: Title: References Destination: blink1 Item: Title: STAR★Methods Destination: flink19 Children: Item: Title: Key resources table Destination: slink20 Item: Title: Experimental model and study participant details Destination: slink21 Children: Item: Title: Inclusion and exclusion criteria for participants Destination: tlink1 Item: Title: Clinical criteria for dementia progression Destination: tlink2 Item: Title: Method details Destination: slink22 Children: Item: Title: DNA extraction Destination: tlink3 Item: Title: Bisulfite treatment Destination: tlink4 Item: Title: Primer design Destination: tlink5 Item: Title: PCR for D-loop and ND1 Destination: tlink6 Item: Title: Amplicon library preparation Destination: tlink7 Item: Title: High-throughput sequencing Destination: tlink8 Item: Title: Quantification and statistical analysis Destination: slink23 Children: Item: Title: Methylation data processing and analysis Destination: tlink9 Item: Title: Bioinformatics workflow for extracting methylation data Destination: tlink10 Item: Title: Exploratory data analysis of methylation patterns Destination: tlink11 Item: Title: Differential methylation analysis Destination: tlink12 Item: Title: Development of the classification model Destination: tlink13 Item: Title: Exploratory data analysis Destination: tlink14 Item: Title: Data preprocessing Destination: tlink15 Item: Title: Model training Destination: tlink16 Item: Title: Model performance evaluation Destination: tlink17 Info: Title: Mitochondrial methylcytosines as blood-based biomarkers for Alzheimer’s disease dementia prognosis Author: Jordi Gascón-Bayarri Subject: ISCIENCE, 28 (2025) 113418. doi:10.1016/j.isci.2025.113418 Creator: Elsevier Producer: PDFlib+PDI 10.0.2p2 (C++/Win64) CreationDate: Thu Sep 04 09:17:25 CEST 2025 ModDate: Thu Sep 18 00:57:34 CEST 2025 ID: 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LastChar: 251 FontDescriptor: FontName: LWFGVS+HelveticaNeue-Bold Flags: Symbolic, ForceBold FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true XMP: application/pdf doi:10.1016/j.isci.2025.113418 Mitochondrial methylcytosines as blood-based biomarkers for Alzheimer’s disease dementia prognosis Jordi Gascón-Bayarri Jose Luis Mosquera Marta Blanch Pau Martí Beatriz Fontal Carla Trapero Nuria Rojo Inma Rico Jaume Campdelacreu Cristopher Fowler Simon M. Laws Adrià Tort-Merino Raquel Sanchez-Valle Joan Bello Juan Fortea Alberto Lleó Courosh Mehanian Russell H. Swerdlow Ramón Reñé-Ramírez Marta Barrachina medicine neurology neuroscience molecular neuroscience ISCIENCE, 28 (2025) 113418. doi:10.1016/j.isci.2025.113418 The Author(s) journal ISCIENCE © 2025 The Author(s). Published by Elsevier Inc. 2589-0042 28 9 19 September 2025 2025-09-19 113418 113418 10.1016/j.isci.2025.113418 https://doi.org/10.1016/j.isci.2025.113418 2010-04-23 true 10.1016/j.isci.2025.113418 elsevier.com sciencedirect.com VoR 7.0.1 10.1016/j.isci.2025.113418 noindex true 2010-04-23 true PStill version 1.84.42 sciencedirect.com elsevier.com Elsevier 2025-09-04T12:17:25+05:30 2025-09-18T03:57:34+05:30 2025-09-18T03:57:34+05:30 True 1 https://www.elsevier.com/tdm/tdmrep-policy.json PDFlib+PDI 10.0.2p2 (C++/Win64) uuid:cb635926-ed9c-4faa-a36e-1ac9016fc55a uuid:0d95b717-543c-497d-bc6f-89f890fed67f http://creativecommons.org/licenses/by-nc-nd/4.0/ Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 354, 496, 530, 506 Flags: Print Annotation: Subtype: Link Rect: 354, 472, 537, 482 Flags: Print Annotation: Subtype: Link Rect: 100, 48, 262, 56 Flags: Print Annotation: Subtype: Link Rect: 53, 58, 69, 74 Flags: Print Page: Label: 2 Annotations: Annotation: Subtype: Link 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