fcc059d104aac938332594dd15f33c65 iSc_a2025v28n1p111537.pdf 13b12dde7613b1b7d6bfe2a424b4a5e863af6d96 iSc_a2025v28n1p111537.pdf fd2c09647e4361813f8bc59aa5cd66dcb087962ec994a0d4981aebba73060ced iSc_a2025v28n1p111537.pdf Title: Post-testicular spermatozoa of a marine teleost can conduct de novo cytoplasmic and mitochondrial translation Subject: ISCIENCE, 28 (2025) 111537. doi:10.1016/j.isci.2024.111537 Author: Júlia Castro-Arnau Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue Jan 14 00:39:52 2025 CET ModDate: Wed Jan 15 15:17:46 2025 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 24 Encrypted: no Page size: 602.986 x 782.986 pts Page rot: 0 File size: 9712442 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NGHCEB+AdvPSHN-H Type 1C WinAnsi yes yes no 1010 0 NGHCGB+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 1012 0 NGHCGC+AdvPSHN-HI Type 1C WinAnsi yes yes no 1014 0 NGHCGD+AdvPSHN-M Type 1C WinAnsi yes yes no 1016 0 NGHCGE+AdvPSMPi6 Type 1C WinAnsi yes yes no 1018 0 NGHCGF+AdvPSHN-MI Type 1C WinAnsi yes yes no 1020 0 NGHDMN+AdvPSSym Type 1C WinAnsi yes yes no 1024 0 NGHDNO+Helvetica-Bold Type 1C WinAnsi yes yes no 1027 0 NGHCGB+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 838 0 NGHCEB+AdvPSHN-H Type 1C MacRoman yes yes no 829 0 NFOKMD+AdvPSA189 Type 1C MacRoman yes yes no 840 0 NFOKME+Advhelvneue-bi Type 1C WinAnsi yes yes no 830 0 NGHCGD+AdvPSHN-M Type 1C Custom yes yes no 813 0 NFOKMG+AdvPSA183 Type 1C MacRoman yes yes no 810 0 NGHCGF+AdvPSHN-MI Type 1C WinAnsi yes yes no 814 0 NFOKNI+Advhelvneue-italic Type 1C WinAnsi yes yes no 824 0 NGHDMN+AdvPSSym Type 1C Custom yes yes yes 821 0 NGHDNO+Helvetica-Bold Type 1C WinAnsi yes yes no 822 0 NFOLJG+AdvP4C4E74 Type 1C Custom yes yes yes 864 0 NFOPHA+AdvPS4731B1 Type 1C WinAnsi yes yes no 867 0 NFOPNO+AdvPS3F4C13 Type 1C WinAnsi yes yes no 871 0 NFPEJC+AdvHelN-L Type 1C WinAnsi yes yes no 878 0 NGHCGC+AdvPSHN-HI Type 1C WinAnsi yes yes no 889 0 NGHCGE+AdvPSMPi6 Type 1C WinAnsi yes yes no 901 0 NGBAAM+AdvPS480274 Type 1C WinAnsi yes yes no 904 0 NGBADM+AdvP4C4E59 Type 1C Custom yes yes yes 916 0 NGBADN+AdvP41CBCA Type 1C WinAnsi yes yes no 917 0 NGBAHN+AdvTimes Type 1C WinAnsi yes yes no 919 0 NGBBBK+AdvP7DED Type 1C WinAnsi yes yes no 930 0 NGBBFK+AdvPS44A44B Type 1C WinAnsi yes yes no 936 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-10-18 03:08:14 CEST RepresentationInformation: iSc_a2025v28n1p111537.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-10-17 17:31:31 CEST Size: 9712442 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 1100 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Post-testicular spermatozoa of a marine teleost can conduct de novo cytoplasmic and mitochondrial translation Destination: titlink1 Children: Item: Title: Introduction Destination: flink1 Item: Title: Results Destination: flink2 Children: Item: Title: Global proteomic analysis of seabream spermatozoa Destination: slink3 Item: Title: Relative quantification of differential protein accumulation during the differentiation and maturation of spermatozoa Destination: slink4 Item: Title: Validation of differentially accumulated proteins in SPZIT and SPZEJ Destination: slink5 Item: Title: Changes of the nuclear-enriched proteome profile during sperm maturation Destination: slink6 Item: Title: Seabream spermatozoa can conduct de novo protein synthesis Destination: slink7 Item: Title: Seabream SPZED can translate nuclear-encoded proteins by cytoplasmic- and mitochondrial-type ribosomes Destination: slink8 Item: Title: Discussion Destination: flink9 Children: Item: Title: Limitations of the study Destination: slink10 Item: Title: Resource availability Destination: flink11 Children: Item: Title: Lead contact Destination: slink12 Item: Title: Materials availability Destination: slink13 Item: Title: Data and code availability Destination: slink14 Item: Title: Acknowledgments Destination: flink15 Item: Title: Author contributions Destination: flink16 Item: Title: Declaration of interests Destination: flink17 Item: Title: Supplemental information Destination: falink18 Item: Title: References Destination: blink1 Item: Title: STAR★Methods Destination: flink19 Children: Item: Title: Key resources table Destination: slink20 Item: Title: Experimental model and study participant details Destination: slink21 Item: Title: Method details Destination: slink22 Children: Item: Title: Sperm motility assays Destination: tlink1 Item: Title: Sample preparation for LC-MS/MS quantitative proteomics Destination: tlink2 Item: Title: Protein digestion, isobaric labeling and fractionation Destination: tlink3 Item: Title: LC-MS/MS analysis Destination: tlink4 Item: Title: Immunofluorescence microscopy and immunoblotting Destination: tlink5 Item: Title: Protein labelling by tRNALys tagged with BODIPY®-FL Destination: tlink6 Item: Title: Sucrose density gradient fractionation of spermatozoa Destination: tlink7 Item: Title: Quantification and statistical analyses Destination: slink23 Children: Item: Title: Protein identification and quantitation by proteomic analysis Destination: tlink8 Item: Title: Protein functional classification and pathway analyses Destination: tlink9 Item: Title: Statistical analyses of sperm kinetic parameters and semiquantitative western blot Destination: tlink10 Info: Title: Post-testicular spermatozoa of a marine teleost can conduct de novo cytoplasmic and mitochondrial translation Author: Júlia Castro-Arnau Subject: ISCIENCE, 28 (2025) 111537. doi:10.1016/j.isci.2024.111537 Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue Jan 14 01:09:52 CET 2025 ModDate: Wed Jan 15 15:47:46 CET 2025 ID: 0x459c8891f400ac7195b986029925709a, 0x660ad53b416a2f4b8d6acfb435b32d78 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1191 ImageHeight: 1191 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 68 ImageHeight: 68 ColorSpace: palette color BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 68 ImageHeight: 68 ColorSpace: palette color BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1994 ImageHeight: 1848 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 2268 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 2264 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 1943 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 1061 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 2375 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 2231 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 1182 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1993 ImageHeight: 1445 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: ICCBased Fonts: Type1: Font: BaseFont: NGHDMN+AdvPSSym FontSubset: true FirstChar: 170 LastChar: 170 FontDescriptor: FontName: NGHDMN+AdvPSSym Flags: Nonsymbolic FontBBox: -177, -291, 1083, 1010 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHDNO+Helvetica-Bold FontSubset: true FirstChar: 108 LastChar: 108 FontDescriptor: FontName: NGHDNO+Helvetica-Bold Flags: Nonsymbolic, ForceBold FontBBox: -170, -228, 1003, 962 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGE+AdvPSMPi6 FontSubset: true FirstChar: 100 LastChar: 100 FontDescriptor: FontName: NGHCGE+AdvPSMPi6 Flags: Nonsymbolic FontBBox: 0, -177, 989, 739 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGB+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 83 LastChar: 110 FontDescriptor: FontName: NGHCGB+HelveticaNeueLTStd-Bd Flags: Nonsymbolic, ForceBold FontBBox: -166, -218, 1078, 975 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFOKMD+AdvPSA189 FontSubset: true FirstChar: 44 LastChar: 223 FontDescriptor: FontName: NFOKMD+AdvPSA189 Flags: Nonsymbolic FontBBox: -156, -208, 1072, 802 FontFile3: true Encoding: MacRomanEncoding Font: BaseFont: NGBAAM+AdvPS480274 FontSubset: true FirstChar: 43 LastChar: 66 FontDescriptor: FontName: NGBAAM+AdvPS480274 Flags: Nonsymbolic FontBBox: 0, -208, 1354, 791 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGBADM+AdvP4C4E59 FontSubset: true FirstChar: 2 LastChar: 160 FontDescriptor: FontName: NGBADM+AdvP4C4E59 Flags: Symbolic FontBBox: -31, -250, 1000, 750 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: NGBADN+AdvP41CBCA FontSubset: true FirstChar: 49 LastChar: 49 FontDescriptor: FontName: NGBADN+AdvP41CBCA Flags: Nonsymbolic FontBBox: -20, -229, 927, 895 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGBAHN+AdvTimes FontSubset: true FirstChar: 45 LastChar: 45 FontDescriptor: FontName: NGBAHN+AdvTimes Flags: Nonsymbolic FontBBox: -217, -212, 1005, 766 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFOLJG+AdvP4C4E74 FontSubset: true FirstChar: 1 LastChar: 48 FontDescriptor: FontName: NFOLJG+AdvP4C4E74 Flags: Symbolic FontBBox: -20, -958, 1114, 770 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: NGBBBK+AdvP7DED FontSubset: true FirstChar: 51 LastChar: 51 FontDescriptor: FontName: NGBBBK+AdvP7DED Flags: Nonsymbolic FontBBox: -20, -229, 927, 770 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFOPHA+AdvPS4731B1 FontSubset: true FirstChar: 37 LastChar: 82 FontDescriptor: FontName: NFOPHA+AdvPS4731B1 Flags: Nonsymbolic FontBBox: 0, -208, 1572, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFOPNO+AdvPS3F4C13 FontSubset: true FirstChar: 97 LastChar: 109 FontDescriptor: FontName: NFOPNO+AdvPS3F4C13 Flags: Nonsymbolic FontBBox: 0, -291, 875, 697 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGBBFK+AdvPS44A44B FontSubset: true FirstChar: 36 LastChar: 36 FontDescriptor: FontName: NGBBFK+AdvPS44A44B Flags: Nonsymbolic FontBBox: 0, -197, 1000, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFOKMG+AdvPSA183 FontSubset: true FirstChar: 37 LastChar: 252 FontDescriptor: FontName: NFOKMG+AdvPSA183 Flags: Nonsymbolic FontBBox: -156, -208, 1072, 791 FontFile3: true Encoding: MacRomanEncoding Font: BaseFont: NGHCGD+AdvPSHN-M FontSubset: true FirstChar: 30 LastChar: 184 FontDescriptor: FontName: NGHCGD+AdvPSHN-M Flags: Nonsymbolic FontBBox: -156, -218, 1062, 802 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: NGHCGF+AdvPSHN-MI FontSubset: true FirstChar: 100 LastChar: 118 FontDescriptor: FontName: NGHCGF+AdvPSHN-MI Flags: Nonsymbolic FontBBox: -156, -208, 1125, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFPEJC+AdvHelN-L FontSubset: true FirstChar: 177 LastChar: 177 FontDescriptor: FontName: NFPEJC+AdvHelN-L Flags: Nonsymbolic FontBBox: -156, -208, 1041, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCEB+AdvPSHN-H FontSubset: true FirstChar: 45 LastChar: 122 FontDescriptor: FontName: NGHCEB+AdvPSHN-H Flags: Nonsymbolic FontBBox: -166, -229, 1093, 812 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGB+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 83 LastChar: 110 FontDescriptor: FontName: NGHCGB+HelveticaNeueLTStd-Bd Flags: Nonsymbolic, ForceBold FontBBox: -166, -218, 1078, 975 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHDMN+AdvPSSym FontSubset: true FirstChar: 2 LastChar: 170 FontDescriptor: FontName: NGHDMN+AdvPSSym Flags: Nonsymbolic FontBBox: -177, -291, 1083, 1010 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: NGHCGC+AdvPSHN-HI FontSubset: true FirstChar: 100 LastChar: 118 FontDescriptor: FontName: NGHCGC+AdvPSHN-HI Flags: Nonsymbolic FontBBox: -156, -218, 1135, 791 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHDNO+Helvetica-Bold FontSubset: true FirstChar: 108 LastChar: 108 FontDescriptor: FontName: NGHDNO+Helvetica-Bold Flags: Nonsymbolic, ForceBold FontBBox: -170, -228, 1003, 962 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGD+AdvPSHN-M FontSubset: true FirstChar: 40 LastChar: 184 FontDescriptor: FontName: NGHCGD+AdvPSHN-M Flags: Nonsymbolic FontBBox: -156, -218, 1062, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NFOKNI+Advhelvneue-italic FontSubset: true FirstChar: 40 LastChar: 121 FontDescriptor: FontName: NFOKNI+Advhelvneue-italic Flags: Nonsymbolic FontBBox: -156, -208, 1104, 791 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGC+AdvPSHN-HI FontSubset: true FirstChar: 67 LastChar: 118 FontDescriptor: FontName: NGHCGC+AdvPSHN-HI Flags: Nonsymbolic FontBBox: -156, -218, 1135, 791 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGE+AdvPSMPi6 FontSubset: true FirstChar: 100 LastChar: 100 FontDescriptor: FontName: NGHCGE+AdvPSMPi6 Flags: Nonsymbolic FontBBox: 0, -177, 989, 739 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCGF+AdvPSHN-MI FontSubset: true FirstChar: 50 LastChar: 118 FontDescriptor: FontName: NGHCGF+AdvPSHN-MI Flags: Nonsymbolic FontBBox: -156, -208, 1125, 802 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: NGHCEB+AdvPSHN-H FontSubset: true FirstChar: 44 LastChar: 223 FontDescriptor: FontName: NGHCEB+AdvPSHN-H Flags: Nonsymbolic FontBBox: -166, -229, 1093, 812 FontFile3: true Encoding: MacRomanEncoding Font: BaseFont: NFOKME+Advhelvneue-bi FontSubset: true FirstChar: 100 LastChar: 118 FontDescriptor: FontName: NFOKME+Advhelvneue-bi Flags: Nonsymbolic FontBBox: -156, -208, 1125, 802 FontFile3: true Encoding: WinAnsiEncoding XMP: application/pdf doi:10.1016/j.isci.2024.111537 Post-testicular spermatozoa of a marine teleost can conduct de novo cytoplasmic and mitochondrial translation Júlia Castro-Arnau François Chauvigné Asier González Roderick Nigel Finn Montserrat Carrascal Joan Cerdà Piscine reproduction Biological sciences Zoology ISCIENCE, 28 (2025) 111537. doi:10.1016/j.isci.2024.111537 The Author(s) journal ISCIENCE © 2024 The Author(s). Published by Elsevier Inc. 2589-0042 28 1 17 January 2025 2025-01-17 111537 111537 10.1016/j.isci.2024.111537 https://doi.org/10.1016/j.isci.2024.111537 2010-04-23 true 10.1016/j.isci.2024.111537 elsevier.com sciencedirect.com VoR 7.0.1 10.1016/j.isci.2024.111537 noindex true 2010-04-23 true sciencedirect.com elsevier.com Elsevier 2025-01-14T05:09:52+05:30 2025-01-15T19:47:46+05:30 2025-01-15T19:47:46+05:30 True 1 https://www.elsevier.com/tdm/tdmrep-policy.json Acrobat Distiller 8.1.0 (Windows) uuid:b1003f8f-44c8-4af5-b89a-eec9c419b43c uuid:ebbc4770-add1-4505-a7c2-cbe64ebaaa3a http://creativecommons.org/licenses/by-nc-nd/4.0/ Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 354, 510, 443, 520 Annotation: Subtype: Link Rect: 100, 48, 262, 56 Annotation: Subtype: Link Rect: 53, 58, 69, 74 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 132, 584, 138, 593 Destination: 1 Annotation: Subtype: Link Rect: 138, 584, 144, 593 Destination: 1 Annotation: Subtype: Link Rect: 144, 584, 147, 593 Destination: 1 Annotation: Subtype: Link Rect: 234, 584, 240, 593 Destination: 1 Annotation: Subtype: Link Rect: 240, 584, 244, 593 Destination: 1 Annotation: Subtype: Link Rect: 310, 584, 314, 593 Destination: 1 Annotation: Subtype: Link Rect: 400, 584, 406, 593 Destination: 1 Annotation: Subtype: Link Rect: 406, 584, 411, 593 Destination: 1 Annotation: Subtype: Link Rect: 411, 584, 415, 593 Destination: 1 Annotation: Subtype: Link Rect: 507, 584, 511, 593 Destination: 1 Annotation: Subtype: Link Rect: 118, 574, 124, 583 Destination: 1 Annotation: Subtype: Link Rect: 124, 574, 130, 583 Destination: 1 Annotation: Subtype: Link Rect: 130, 574, 135, 583 Destination: 1 Annotation: Subtype: Link Rect: 135, 574, 139, 583 Destination: 1 Annotation: Subtype: Link Rect: 119, 495, 188, 502 Annotation: Subtype: Link Rect: 53, 485, 201, 493 Annotation: Subtype: Link Rect: 160, 213, 164, 221 Destination: 412 Annotation: Subtype: Link Rect: 166, 213, 169, 221 Destination: 412 Annotation: Subtype: Link Rect: 190, 169, 193, 177 Destination: 412 Annotation: Subtype: Link Rect: 195, 147, 198, 155 Destination: 412 Annotation: Subtype: Link Rect: 200, 147, 204, 155 Destination: 412 Annotation: Subtype: Link Rect: 185, 125, 188, 133 Destination: 412 Annotation: Subtype: Link Rect: 190, 125, 194, 133 Destination: 412 Annotation: Subtype: Link Rect: 96, 81, 99, 90 Destination: 412 Annotation: Subtype: Link Rect: 101, 81, 105, 90 Destination: 412 Annotation: Subtype: Link Rect: 505, 278, 508, 287 Destination: 412 Annotation: Subtype: Link Rect: 331, 235, 341, 243 Destination: 412 Annotation: Subtype: Link Rect: 345, 213, 352, 221 Destination: 412 Annotation: Subtype: Link Rect: 356, 202, 359, 210 Destination: 412 Annotation: Subtype: Link Rect: 397, 169, 414, 177 Destination: 412 Annotation: Subtype: Link Rect: 357, 125, 364, 133 Destination: 412 Annotation: Subtype: Link Rect: 366, 125, 373, 133 Destination: 412 Annotation: Subtype: Link Rect: 375, 125, 382, 133 Destination: 412 Annotation: Subtype: Link Rect: 384, 125, 391, 133 Destination: 412 Annotation: Subtype: Link Rect: 430, 70, 433, 79 Destination: 412 Annotation: Subtype: Link Rect: 53, 16, 69, 32 Annotation: Subtype: Link Rect: 334, 10, 516, 18 Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 286, 665, 290, 674 Destination: 412 Annotation: Subtype: Link Rect: 291, 665, 298, 674 Destination: 488 Annotation: Subtype: Link Rect: 139, 611, 156, 619 Destination: 488 Annotation: Subtype: Link Rect: 213, 611, 220, 619 Destination: 488 Annotation: Subtype: Link Rect: 222, 611, 229, 619 Destination: 488 Annotation: Subtype: Link Rect: 76, 578, 93, 586 Destination: 488 Annotation: Subtype: Link Rect: 248, 556, 255, 564 Destination: 488 Annotation: Subtype: Link Rect: 256, 556, 263, 564 Destination: 488 Annotation: Subtype: Link Rect: 291, 315, 298, 323 Destination: 488 Annotation: Subtype: Link Rect: 61, 300, 92, 308 Destination: 118 Annotation: Subtype: Link Rect: 216, 267, 246, 276 Destination: 118 Annotation: Subtype: Link Rect: 61, 169, 92, 177 Destination: 118 Annotation: Subtype: Link Rect: 257, 117, 264, 126 Destination: 488 Annotation: Subtype: Link Rect: 284, 92, 298, 100 Destination: 118 Annotation: Subtype: Link Rect: 59, 81, 78, 89 Destination: 118 Annotation: Subtype: Link Rect: 239, 48, 271, 56 Destination: 118 Annotation: Subtype: Link Rect: 377, 651, 409, 659 Destination: 118 Annotation: Subtype: Link Rect: 461, 618, 493, 626 Destination: 118 Annotation: Subtype: Link Rect: 449, 508, 482, 517 Destination: 412 Annotation: Subtype: Link Rect: 467, 490, 474, 499 Destination: 488 Annotation: Subtype: Link Rect: 371, 479, 378, 488 Destination: 488 Annotation: Subtype: Link Rect: 358, 388, 389, 396 Destination: 118 Annotation: Subtype: Link Rect: 415, 388, 449, 396 Destination: 412 Annotation: Subtype: Link Rect: 454, 311, 485, 319 Destination: 118 Annotation: Subtype: Link Rect: 511, 311, 545, 319 Destination: 412 Annotation: Subtype: Link Rect: 349, 256, 379, 265 Destination: 118 Annotation: Subtype: Link Rect: 402, 256, 435, 265 Destination: 412 Annotation: Subtype: Link Rect: 350, 190, 381, 199 Destination: 118 Annotation: Subtype: Link Rect: 404, 190, 438, 199 Destination: 412 Annotation: Subtype: Link Rect: 475, 92, 511, 100 Destination: 412 Page: Label: 4 Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 120, 622, 127, 630 Destination: 488 Annotation: Subtype: Link Rect: 137, 519, 172, 528 Destination: 173 Annotation: Subtype: Link Rect: 198, 519, 232, 528 Destination: 412 Annotation: Subtype: Link Rect: 145, 490, 152, 499 Destination: 488 Annotation: Subtype: Link Rect: 222, 432, 259, 440 Destination: 173 Annotation: Subtype: Link Rect: 59, 421, 93, 429 Destination: 412 Annotation: Subtype: Link Rect: 260, 355, 292, 363 Destination: 173 Annotation: Subtype: Link Rect: 75, 344, 108, 352 Destination: 412 Annotation: Subtype: Link Rect: 209, 311, 240, 319 Destination: 173 Annotation: Subtype: Link Rect: 261, 311, 294, 319 Destination: 412 Annotation: Subtype: Link Rect: 159, 267, 189, 276 Destination: 173 Annotation: Subtype: Link Rect: 212, 267, 245, 276 Destination: 412 Annotation: Subtype: Link Rect: 139, 234, 170, 243 Destination: 173 Annotation: Subtype: Link Rect: 195, 234, 228, 243 Destination: 412 Annotation: Subtype: Link Rect: 80, 158, 111, 166 Destination: 188 Annotation: Subtype: Link Rect: 134, 158, 168, 166 Destination: 412 Annotation: Subtype: Link Rect: 284, 92, 298, 100 Destination: 188 Annotation: Subtype: Link Rect: 59, 81, 80, 89 Destination: 188 Annotation: Subtype: Link Rect: 108, 81, 143, 89 Destination: 412 Annotation: Subtype: Link Rect: 192, 52, 199, 60 Destination: 488 Annotation: Subtype: Link Rect: 542, 644, 549, 652 Destination: 488 Annotation: Subtype: Link Rect: 313, 541, 342, 550 Destination: 188 Annotation: Subtype: Link Rect: 421, 464, 452, 473 Destination: 188 Annotation: Subtype: Link Rect: 402, 136, 435, 144 Destination: 204 Annotation: Subtype: Link Rect: 371, 103, 401, 111 Destination: 204 Annotation: Subtype: Link Rect: 431, 70, 461, 78 Destination: 204 Annotation: Subtype: Link Rect: 508, 48, 539, 56 Destination: 204 Page: Label: 6 Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 193, 97, 218, 104 Destination: 173 Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 148, 70, 179, 78 Destination: 204 Annotation: Subtype: Link Rect: 488, 48, 518, 56 Destination: 204 Page: Label: 9 Annotations: Annotation: Subtype: Link Rect: 81, 169, 112, 177 Destination: 118 Annotation: Subtype: Link Rect: 173, 136, 205, 144 Destination: 221 Annotation: Subtype: Link Rect: 139, 59, 175, 67 Destination: 412 Annotation: Subtype: Link Rect: 313, 212, 345, 221 Destination: 221 Annotation: Subtype: Link Rect: 357, 212, 396, 221 Destination: 412 Annotation: Subtype: Link Rect: 418, 212, 428, 221 Destination: 412 Annotation: Subtype: Link Rect: 313, 158, 344, 166 Destination: 221 Annotation: Subtype: Link Rect: 313, 114, 343, 122 Destination: 221 Annotation: Subtype: Link Rect: 379, 81, 410, 89 Destination: 221 Annotation: Subtype: Link Rect: 435, 81, 469, 89 Destination: 412 Page: Label: 10 Page: Label: 11 Annotations: Annotation: Subtype: Link Rect: 261, 585, 292, 593 Destination: 221 Annotation: Subtype: Link Rect: 78, 574, 114, 582 Destination: 412 Annotation: Subtype: Link Rect: 284, 541, 298, 550 Destination: 221 Annotation: Subtype: Link Rect: 59, 530, 80, 539 Destination: 221 Annotation: Subtype: Link Rect: 217, 475, 248, 484 Destination: 221 Annotation: Subtype: Link Rect: 291, 435, 298, 444 Destination: 488 Annotation: Subtype: Link Rect: 184, 399, 216, 407 Destination: 246 Annotation: Subtype: Link Rect: 61, 245, 93, 254 Destination: 246 Annotation: Subtype: Link Rect: 533, 567, 540, 575 Destination: 488 Annotation: Subtype: Link Rect: 492, 519, 527, 528 Destination: 291 Annotation: Subtype: Link Rect: 437, 453, 472, 462 Destination: 291 Annotation: Subtype: Link Rect: 313, 410, 347, 418 Destination: 291 Annotation: Subtype: Link Rect: 313, 355, 347, 363 Destination: 291 Annotation: Subtype: Link Rect: 462, 355, 498, 363 Destination: 412 Annotation: Subtype: Link Rect: 427, 234, 462, 243 Destination: 412 Annotation: Subtype: Link Rect: 385, 158, 416, 166 Destination: 332 Annotation: Subtype: Link Rect: 368, 125, 399, 133 Destination: 332 Page: Label: 12 Page: Label: 13 Annotations: Annotation: Subtype: Link Rect: 61, 651, 93, 659 Destination: 332 Annotation: Subtype: Link Rect: 258, 607, 288, 615 Destination: 332 Annotation: Subtype: Link Rect: 61, 366, 92, 374 Destination: 332 Annotation: Subtype: Link Rect: 156, 245, 188, 254 Destination: 332 Annotation: Subtype: Link Rect: 191, 212, 228, 221 Destination: 332 Annotation: Subtype: Link Rect: 206, 117, 213, 126 Destination: 488 Annotation: Subtype: Link Rect: 218, 114, 255, 122 Destination: 412 Annotation: Subtype: Link Rect: 457, 563, 492, 572 Destination: 356 Annotation: Subtype: Link Rect: 428, 497, 463, 506 Destination: 356 Annotation: Subtype: Link Rect: 392, 381, 399, 389 Destination: 488 Annotation: Subtype: Link Rect: 495, 359, 502, 367 Destination: 488 Annotation: Subtype: Link Rect: 437, 333, 472, 341 Destination: 356 Annotation: Subtype: Link Rect: 508, 300, 540, 309 Destination: 356 Page: Label: 14 Annotations: Annotation: Subtype: Link Rect: 186, 96, 193, 104 Destination: 488 Annotation: Subtype: Link Rect: 73, 63, 91, 71 Destination: 488 Annotation: Subtype: Link Rect: 504, 216, 511, 225 Destination: 488 Annotation: Subtype: Link Rect: 536, 183, 543, 192 Destination: 488 Annotation: Subtype: Link Rect: 333, 85, 340, 93 Destination: 488 Annotation: Subtype: Link Rect: 364, 63, 371, 71 Destination: 488 Annotation: Subtype: Link Rect: 401, 63, 408, 71 Destination: 488 Annotation: Subtype: Link Rect: 437, 63, 444, 71 Destination: 591 Annotation: Subtype: Link Rect: 481, 63, 488, 71 Destination: 591 Page: Label: 15 Annotations: Annotation: Subtype: Link Rect: 238, 183, 256, 192 Destination: 591 Annotation: Subtype: Link Rect: 160, 128, 167, 137 Destination: 591 Annotation: Subtype: Link Rect: 169, 128, 176, 137 Destination: 591 Annotation: Subtype: Link Rect: 137, 74, 144, 82 Destination: 412 Annotation: Subtype: Link Rect: 146, 74, 153, 82 Destination: 591 Annotation: Subtype: Link Rect: 445, 238, 452, 247 Destination: 591 Annotation: Subtype: Link Rect: 359, 216, 366, 225 Destination: 412 Annotation: Subtype: Link Rect: 368, 216, 385, 225 Destination: 412 Page: Label: 16 Annotations: Annotation: Subtype: Link Rect: 253, 479, 260, 488 Destination: 412 Annotation: Subtype: Link Rect: 261, 479, 268, 488 Destination: 591 Annotation: Subtype: Link Rect: 184, 435, 191, 444 Destination: 488 Annotation: Subtype: Link Rect: 259, 304, 266, 312 Destination: 412 Annotation: Subtype: Link Rect: 194, 293, 201, 301 Destination: 591 Annotation: Subtype: Link Rect: 141, 249, 158, 258 Destination: 591 Annotation: Subtype: Link Rect: 234, 205, 241, 214 Destination: 591 Annotation: Subtype: Link Rect: 135, 161, 142, 170 Destination: 591 Annotation: Subtype: Link Rect: 116, 85, 123, 93 Destination: 591 Annotation: Subtype: Link Rect: 125, 85, 132, 93 Destination: 591 Annotation: Subtype: Link Rect: 253, 74, 261, 82 Destination: 591 Annotation: Subtype: Link Rect: 217, 63, 224, 71 Destination: 591 Annotation: Subtype: Link Rect: 490, 600, 497, 608 Destination: 591 Annotation: Subtype: Link Rect: 499, 600, 506, 608 Destination: 591 Annotation: Subtype: Link Rect: 505, 159, 543, 166 Annotation: Subtype: Link Rect: 304, 150, 325, 157 Annotation: Subtype: Link Rect: 473, 80, 479, 87 Destination: 591 Annotation: Subtype: Link Rect: 394, 58, 455, 65 Destination: 672 Page: Label: 17 Annotations: Annotation: Subtype: Link Rect: 163, 665, 202, 672 Destination: 379 Annotation: Subtype: Link Rect: 79, 337, 160, 344 Destination: 672 Annotation: Subtype: Link Rect: 79, 328, 275, 335 Destination: 686 Annotation: Subtype: Link Rect: 79, 318, 138, 325 Destination: 686 Annotation: Subtype: Link Rect: 79, 242, 235, 249 Destination: 767 Annotation: Subtype: Link Rect: 208, 163, 298, 170 Annotation: Subtype: Link Rect: 59, 154, 100, 161 Annotation: Subtype: Link Rect: 155, 48, 293, 55 Annotation: Subtype: Link Rect: 375, 665, 505, 672 Annotation: Subtype: Link Rect: 447, 642, 549, 649 Annotation: Subtype: Link Rect: 323, 633, 354, 640 Annotation: Subtype: Link Rect: 447, 601, 549, 608 Annotation: Subtype: Link Rect: 323, 592, 363, 599 Annotation: Subtype: Link Rect: 493, 570, 549, 577 Annotation: Subtype: Link Rect: 323, 560, 396, 567 Annotation: Subtype: Link Rect: 493, 538, 549, 545 Annotation: Subtype: Link Rect: 323, 529, 385, 536 Annotation: Subtype: Link Rect: 323, 497, 465, 504 Annotation: Subtype: Link Rect: 370, 447, 503, 453 Annotation: Subtype: Link Rect: 323, 396, 463, 403 Annotation: Subtype: Link Rect: 447, 364, 549, 371 Annotation: Subtype: Link Rect: 323, 355, 348, 362 Annotation: Subtype: Link Rect: 475, 333, 549, 340 Annotation: Subtype: Link Rect: 323, 323, 389, 330 Annotation: Subtype: Link Rect: 354, 292, 465, 299 Annotation: Subtype: Link Rect: 354, 260, 479, 267 Annotation: Subtype: Link Rect: 515, 219, 549, 226 Annotation: Subtype: Link Rect: 323, 209, 415, 216 Annotation: Subtype: Link Rect: 450, 168, 549, 175 Annotation: Subtype: Link Rect: 323, 159, 359, 166 Annotation: Subtype: Link Rect: 475, 118, 549, 125 Annotation: Subtype: Link Rect: 323, 108, 379, 115 Annotation: Subtype: Link Rect: 461, 58, 549, 65 Annotation: Subtype: Link Rect: 323, 48, 364, 55 Page: Label: 18 Annotations: Annotation: Subtype: Link Rect: 269, 665, 292, 672 Annotation: Subtype: Link Rect: 65, 655, 191, 662 Annotation: Subtype: Link Rect: 245, 623, 292, 630 Annotation: Subtype: Link Rect: 65, 613, 145, 620 Annotation: Subtype: Link Rect: 235, 581, 292, 588 Annotation: Subtype: Link Rect: 65, 571, 145, 578 Annotation: Subtype: Link Rect: 217, 539, 292, 546 Annotation: Subtype: Link Rect: 65, 529, 133, 536 Annotation: Subtype: Link Rect: 88, 497, 221, 504 Annotation: Subtype: Link Rect: 269, 455, 292, 462 Annotation: Subtype: Link Rect: 65, 445, 183, 452 Annotation: Subtype: Link Rect: 141, 413, 259, 420 Annotation: Subtype: Link Rect: 96, 361, 260, 368 Annotation: Subtype: Link Rect: 258, 329, 292, 336 Annotation: Subtype: Link Rect: 65, 319, 184, 326 Annotation: Subtype: Link Rect: 214, 287, 292, 294 Annotation: Subtype: Link Rect: 65, 277, 120, 284 Annotation: Subtype: Link Rect: 178, 235, 292, 242 Annotation: Subtype: Link Rect: 65, 226, 91, 233 Annotation: Subtype: Link Rect: 186, 193, 292, 200 Annotation: Subtype: Link Rect: 65, 184, 127, 191 Annotation: Subtype: Link Rect: 217, 151, 292, 158 Annotation: Subtype: Link Rect: 65, 142, 158, 149 Annotation: Subtype: Link Rect: 117, 90, 256, 97 Annotation: Subtype: Link Rect: 179, 58, 292, 65 Annotation: Subtype: Link Rect: 65, 48, 98, 55 Annotation: Subtype: Link Rect: 420, 655, 522, 662 Annotation: Subtype: Link Rect: 336, 614, 478, 621 Annotation: Subtype: Link Rect: 316, 582, 458, 589 Annotation: Subtype: Link Rect: 423, 541, 539, 548 Annotation: Subtype: Link Rect: 384, 491, 502, 498 Annotation: Subtype: Link Rect: 337, 450, 454, 457 Annotation: Subtype: Link Rect: 423, 409, 541, 416 Annotation: Subtype: Link Rect: 316, 368, 445, 375 Annotation: Subtype: Link Rect: 355, 327, 474, 333 Annotation: Subtype: Link Rect: 509, 285, 543, 292 Annotation: Subtype: Link Rect: 316, 276, 386, 283 Annotation: Subtype: Link Rect: 520, 254, 543, 261 Annotation: Subtype: Link Rect: 316, 244, 402, 251 Annotation: Subtype: Link Rect: 486, 213, 543, 220 Annotation: Subtype: Link Rect: 316, 203, 387, 210 Annotation: Subtype: Link Rect: 383, 172, 518, 179 Annotation: Subtype: Link Rect: 375, 140, 478, 147 Annotation: Subtype: Link Rect: 410, 89, 540, 96 Annotation: Subtype: Link Rect: 328, 48, 473, 55 Page: Label: 19 Annotations: Annotation: Subtype: Link Rect: 122, 646, 282, 653 Annotation: Subtype: Link Rect: 72, 606, 189, 613 Annotation: Subtype: Link Rect: 185, 566, 298, 573 Annotation: Subtype: Link Rect: 72, 557, 93, 564 Annotation: Subtype: Link Rect: 242, 526, 298, 533 Annotation: Subtype: Link Rect: 72, 517, 171, 524 Annotation: Subtype: Link Rect: 242, 486, 298, 493 Annotation: Subtype: Link Rect: 72, 477, 123, 484 Annotation: Subtype: Link Rect: 134, 437, 264, 444 Annotation: Subtype: Link Rect: 242, 397, 298, 404 Annotation: Subtype: Link Rect: 72, 388, 135, 395 Annotation: Subtype: Link Rect: 176, 367, 298, 374 Annotation: Subtype: Link Rect: 72, 357, 98, 364 Annotation: Subtype: Link Rect: 72, 327, 204, 334 Annotation: Subtype: Link Rect: 147, 297, 270, 303 Annotation: Subtype: Link Rect: 242, 276, 298, 283 Annotation: Subtype: Link Rect: 72, 266, 129, 273 Annotation: Subtype: Link Rect: 398, 665, 540, 672 Annotation: Subtype: Link Rect: 410, 634, 529, 641 Annotation: Subtype: Link Rect: 439, 604, 549, 611 Annotation: Subtype: Link Rect: 323, 594, 341, 601 Annotation: Subtype: Link Rect: 361, 555, 470, 561 Annotation: Subtype: Link Rect: 435, 515, 549, 522 Annotation: Subtype: Link Rect: 323, 505, 350, 512 Annotation: Subtype: Link Rect: 527, 484, 549, 491 Annotation: Subtype: Link Rect: 323, 475, 417, 482 Annotation: Subtype: Link Rect: 386, 435, 496, 442 Annotation: Subtype: Link Rect: 391, 386, 509, 393 Annotation: Subtype: Link Rect: 369, 346, 474, 353 Annotation: Subtype: Link Rect: 389, 306, 534, 313 Annotation: Subtype: Link Rect: 323, 295, 549, 302 Annotation: Subtype: Link Rect: 323, 285, 549, 292 Annotation: Subtype: Link Rect: 323, 276, 361, 283 Annotation: Subtype: Link Rect: 354, 245, 465, 252 Page: Label: 18.e1 Sequence: 20 Annotations: Annotation: Subtype: Link Rect: 257, 459, 263, 467 Destination: 591 Page: Label: 18.e2 Sequence: 21 Annotations: Annotation: Subtype: Link Rect: 312, 224, 319, 232 Destination: 591 Annotation: Subtype: Link Rect: 151, 202, 158, 210 Destination: 488 Annotation: Subtype: Link Rect: 418, 92, 425, 101 Destination: 488 Annotation: Subtype: Link Rect: 427, 92, 434, 101 Destination: 591 Annotation: Subtype: Link Rect: 516, 637, 522, 644 Destination: 591 Annotation: Subtype: Link Rect: 67, 554, 101, 561 Destination: 412 Annotation: Subtype: Link Rect: 433, 517, 537, 525 Annotation: Subtype: Link Rect: 365, 507, 528, 515 Annotation: Subtype: Link Rect: 433, 495, 537, 503 Annotation: Subtype: Link Rect: 365, 485, 528, 493 Annotation: Subtype: Link Rect: 431, 473, 511, 481 Annotation: Subtype: Link Rect: 264, 465, 270, 472 Destination: 488 Annotation: Subtype: Link Rect: 404, 461, 526, 469 Annotation: Subtype: Link Rect: 299, 453, 305, 460 Destination: 488 Annotation: Subtype: Link Rect: 404, 449, 532, 457 Annotation: Subtype: Link Rect: 365, 439, 412, 447 Annotation: Subtype: Link Rect: 433, 427, 509, 435 Annotation: Subtype: Link Rect: 365, 417, 396, 425 Annotation: Subtype: Link Rect: 433, 406, 520, 413 Annotation: Subtype: Link Rect: 433, 394, 512, 401 Annotation: Subtype: Link Rect: 431, 382, 519, 389 Annotation: Subtype: Link Rect: 433, 370, 501, 377 Annotation: Subtype: Link Rect: 431, 358, 503, 365 Annotation: Subtype: Link Rect: 433, 346, 517, 353 Annotation: Subtype: Link Rect: 433, 334, 510, 341 Annotation: Subtype: Link Rect: 365, 324, 422, 331 Annotation: Subtype: Link Rect: 433, 312, 516, 319 Annotation: Subtype: Link Rect: 365, 302, 517, 309 Annotation: Subtype: Link Rect: 433, 290, 518, 297 Annotation: Subtype: Link Rect: 365, 280, 505, 288 Page: Label: 18.e3 Sequence: 22 Annotations: Annotation: Subtype: Link Rect: 204, 676, 211, 685 Destination: 591 Annotation: Subtype: Link Rect: 480, 633, 487, 641 Destination: 488 Annotation: Subtype: Link Rect: 204, 446, 211, 455 Destination: 591 Page: Label: 18.e4 Sequence: 23 Annotations: Annotation: Subtype: Link Rect: 342, 665, 349, 674 Destination: 488 Annotation: Subtype: Link Rect: 411, 644, 418, 652 Destination: 591 Annotation: Subtype: Link Rect: 334, 607, 367, 615 Destination: 412 Annotation: Subtype: Link Rect: 291, 432, 324, 440 Destination: 412 Annotation: Subtype: Link Rect: 61, 234, 99, 243 Destination: 412 Annotation: Subtype: Link Rect: 195, 238, 202, 247 Destination: 591 Annotation: Subtype: Link Rect: 439, 128, 446, 137 Destination: 488 Page: Label: 18.e5 Sequence: 24 Annotations: Annotation: Subtype: Link Rect: 298, 644, 305, 652 Destination: 488 Checksum: 43dc3a75 Type: CRC32 Checksum: fcc059d104aac938332594dd15f33c65 Type: MD5 Checksum: 13b12dde7613b1b7d6bfe2a424b4a5e863af6d96 Type: SHA-1 Checksum: fd2c09647e4361813f8bc59aa5cd66dcb087962ec994a0d4981aebba73060ced Type: SHA-256