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Title:           Application of Combined Irradiation Mutagenesis Technique for Hyperproduction of Surfactin in Bacillus velezensis Strain AF_3B
Subject:         International Journal of Microbiology 2025.2025:5570585
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     Item: 
      Title: Application of Combined Irradiation Mutagenesis Technique for Hyperproduction of Surfactin in Bacillus velezensis Strain AF_3B
      Destination: maintitle
      Children: 
       Item: 
        Title: 1. Introduction
        Destination: section1
       Item: 
        Title: 2. Experimental Procedures
        Destination: section2
        Children: 
         Item: 
          Title: 2.1. Microorganisms and Medium Composition
          Destination: section3
         Item: 
          Title: 2.2. Screening of Purified Isolates for the Production of LP
          Destination: section4
          Children: 
           Item: 
            Title: 2.2.1. Hemolytic Activity
            Destination: section5
           Item: 
            Title: 2.2.2. Oil Spreading Technique
            Destination: section6
           Item: 
            Title: 2.2.3. Emulsification Assay (E24)
            Destination: section7
           Item: 
            Title: 2.2.4. Drop Collapse Assay
            Destination: section8
           Item: 
            Title: 2.2.5. Thin Layer Chromatography (TLC)
            Destination: section9
         Item: 
          Title: 2.3. Identification of Selected Isolates
          Destination: section10
         Item: 
          Title: 2.4. Mutant Development
          Destination: section11
          Children: 
           Item: 
            Title: 2.4.1. UV Irradiation Mutagenesis
            Destination: section12
           Item: 
            Title: 2.4.2. Determination of Survival Ratios
            Destination: section13
           Item: 
            Title: 2.4.3. Determination of Antifungal Activity
            Destination: section14
           Item: 
            Title: 2.4.4. γ-Irradiation Mutagenesis
            Destination: section15
         Item: 
          Title: 2.5. Screening of Selected Survivors With Enhanced Surfactin Yield
          Destination: section16
         Item: 
          Title: 2.6. Optimization of Different Fermentation Parameters
          Destination: section17
          Children: 
           Item: 
            Title: 2.6.1. Effect of Carbon and Nitrogen Sources
            Destination: section18
           Item: 
            Title: 2.6.2. Effect of pH and Temperature
            Destination: section19
           Item: 
            Title: 2.6.3. Effect of Inoculum Size (V/V)
            Destination: section20
           Item: 
            Title: 2.6.4. Effect of Incubation Period
            Destination: section21
         Item: 
          Title: 2.7. Evaluation of Different Agroindustrial Wastes as Substrate
          Destination: section22
         Item: 
          Title: 2.8. Extraction and Purification of LP-Surfactin
          Destination: section23
         Item: 
          Title: 2.9. Purification of LPs by Column Chromatography
          Destination: section24
         Item: 
          Title: 2.10. Identification of Surfactin by Mass Spectrometric Analysis
          Destination: section25
          Children: 
           Item: 
            Title: 2.10.1. Matrix-Assisted Laser Desorption/Ionization Time of Flight
            Destination: section26
       Item: 
        Title: 3. Results
        Destination: section27
        Children: 
         Item: 
          Title: 3.1. Isolation and Identification
          Destination: section28
         Item: 
          Title: 3.2. Screening for LP Production
          Destination: section29
         Item: 
          Title: 3.3. Irradiation Mutagenesis
          Destination: section30
         Item: 
          Title: 3.4. Effects of Irradiation Mutagenesis on Colony Morphologies
          Destination: section31
         Item: 
          Title: 3.5. Screening for the Production of LPs
          Destination: section32
          Children: 
           Item: 
            Title: 3.5.1. Hemolytic Activity
            Destination: section33
           Item: 
            Title: 3.5.2. Drop Collapse Assay
            Destination: section34
           Item: 
            Title: 3.5.3. Oil Displacement Activity
            Destination: section35
           Item: 
            Title: 3.5.4. Emulsification Activity
            Destination: section36
           Item: 
            Title: 3.5.5. Antifungal Activity
            Destination: section37
         Item: 
          Title: 3.6. Optimization Studies
          Destination: section38
          Children: 
           Item: 
            Title: 3.6.1. Effect of Carbon and Nitrogen Sources
            Destination: section39
           Item: 
            Title: 3.6.2. Effect of pH, Temperature, Inoculum Size, and Incubation Period
            Destination: section40
         Item: 
          Title: 3.7. Evaluation of Different Agroindustrial Wastes as Substrate
          Destination: section41
         Item: 
          Title: 3.8. Identification of LPs
          Destination: section42
       Item: 
        Title: 4. Discussion
        Destination: section43
       Item: 
        Title: 5. Conclusions
        Destination: section44
       Item: 
        Title: Data Availability Statement
        Destination: section45
       Item: 
        Title: Conflicts of Interest
        Destination: section46
       Item: 
        Title: Author Contributions
        Destination: section47
       Item: 
        Title: Funding
        Destination: section48
       Item: 
        Title: Acknowledgments
        Destination: section49
       Item: 
        Title: Supporting Information
        Destination: section50
       Item: 
        Title: References
        Destination: section51
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